2',4,4',5',7,7'-Hexachloro-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 英文名称: 2',4,4',5',7,7'-Hexachloro-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
• 化学式: C₂₀H₆Cl₆O₅
• 分子量: 539.0
• InChiKey: KSSBSUNQTOAQKI-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  7.2
• 重原子数：
  31
• 可旋转键数：
  0
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.05
• 拓扑面积：
  76
• 氢给体数：
  2
• 氢受体数：
  5

文献信息

• Spectral calibration of fluorescent polynucleotide separation apparatus
  申请人：PE Corporation (NY)

  公开号：EP1178305A2

  公开（公告）日：2002-02-06

  The invention relates to methods, compositions, and systems for calibrating a fluorescent polynucleotide separation apparatus. One aspect of the invention is multiple color calibration standards and their use. A multiple color calibration standard is a mixture of at least two polynucleotide of different length, wherein each of the polynucleotide is labeled with a spectrally distinct fluorescent dye. Another aspect of the invention is to produce total emission temporal profiles of multiple color calibration standards for use in calibrating fluorescent polynucleotide separation apparatus. The peaks corresponding to the fluorescently labeled polynucleotide in the total emission temporal profile may be detected using a peak detector that is driven by changes in the slopes of the total emission temporal profile. Calibration of fluorescent polynucleotide separation apparatus with various embodiments ofthe methods ofthe invention include the step of identification of the labeled polynucleotide of the multiple color calibration standards. The process of spectral calibration of fluorescent polynucleotide separation apparatus using multiple color calibration standard may include the step of the estimating (extracting) of the dyes' reference spectra, using information from the peak detection process performed on the total emission temporal profile. Other aspects of the invention include systems for separating and detecting fluorescently labeled polynucleotide, wherein the system is designed for spectral calibration in accordance with the subject calibration methods employing multiple color calibration standards. Another aspect of the invention is methods and compositions for detecting the flow of electrical current through a separation channel of a fluorescent polynucleotide separation apparatus. These methods an compositions employ monitoring dyes. Monitoring dyes are fluorescent dyes that are spectrally distinct from the dye on the polynucleotide intended to convey genetic information, e.g., fluorescent polynucleotide sequencing reaction products.

  本发明涉及校准荧光多核苷酸分离装置的方法、组合物和系统。本发明的一个方面是多色校准标准及其使用。多色校准标准是至少两种不同长度的多核苷酸的混合物，其中每种多核苷酸都用光谱不同的荧光染料标记。本发明的另一方面是制作多色校准标准的总发射时间曲线，用于校准荧光多核苷酸分离装置。总发射时间曲线中对应于荧光标记多核苷酸的峰值可使用峰值检测器进行检测，该检测器由总发射时间曲线斜率的变化驱动。用本发明方法的各种实施例校准荧光多核苷酸分离装置，包括识别多色校准标准中的标记多核苷酸的步骤。使用多色校准标准对荧光多核苷酸分离装置进行光谱校准的过程可包括利用在总发射时间曲线上执行的峰值检测过程中获得的信息估算（提取）染料参考光谱的步骤。本发明的其他方面包括用于分离和检测荧光标记多核苷酸的系统，其中该系统是根据采用多色校准标准的主题校准方法设计的光谱校准系统。本发明的另一方面是检测电流流经荧光多核苷酸分离装置的分离通道的方法和组合物。这些方法和组合物采用监测染料。监测染料是一种荧光染料，在光谱上与多核苷酸上的染料（如荧光多核苷酸测序反应产物）不同，后者旨在传递遗传信息。
• Probes and methods for hepatitis C virus typing using multidimensional probe analysis
  申请人：Roche Diagnostics GmbH

  公开号：EP1746170A2

  公开（公告）日：2007-01-24

  This invention provides compositions and methods for HCV typing, e.g., genotyping and/or subtyping. The compositions and methods of the invention can be used to assign an HCV isolate to one of at least five HCV types (for example, selected from types 1, 2, 3, 4, 5 or 6), or to one of at least five subtypes (for example, subtypes 1a/b/c, 2a/c, 2b, 3a, 4a, 5a or 6a). These methods integrate the hybridization data from a plurality of HCV typing probes in a multidimensional analysis to make an HCV type assignment for an HCV in an experimental sample. The invention also provides related compositions, including, for example, the HCV typing probes and HCV typing diagnostic kits.

  本发明提供了用于 HCV 分型（如基因分型和/或亚型）的组合物和方法。本发明的组合物和方法可用于将 HCV 分离物归入至少五种 HCV 类型之一（例如，选自 1、2、3、4、5 或 6 型），或至少五种亚型之一（例如，1a/b/c、2a/c、2b、3a、4a、5a 或 6a 亚型）。这些方法在多维分析中整合了来自多个 HCV 分型探针的杂交数据，从而对实验样本中的 HCV 进行 HCV 分型。本发明还提供了相关组合物，例如包括 HCV 分型探针和 HCV 分型诊断试剂盒。
• METHOD FOR DETECTING FISH PATHOGEN
  申请人：Schweitzer Biotech Company Ltd.

  公开号：EP3862443A1

  公开（公告）日：2021-08-11

  A method for detecting a fish pathogen, and an oligonucleotide pair for detecting a fish pathogen.

  一种检测鱼类病原体的方法和一种检测鱼类病原体的寡核苷酸对。
• Methods and reagents for detection, quantitation, and serotyping of dengue viruses
  申请人：The Board of Trustees of the Leland Stanford Junior University

  公开号：US10093994B2

  公开（公告）日：2018-10-09

  Methods and oligonucleotide reagents for diagnosing dengue virus infection are described. In particular, the invention relates to methods for detection, quantitation, and serotyping dengue virus, including serotypes 1-4. The dengue virus can be specifically detected by these methods even in samples containing other viruses, such as West Nile virus, Japanese encephalitis virus, tick-born encephalitis virus, HIV, or HCV.

  本发明描述了诊断登革病毒感染的方法和寡核苷酸试剂。本发明尤其涉及登革病毒（包括 1-4 血清型）的检测、定量和血清分型方法。即使样本中含有其他病毒，如西尼罗河病毒、日本脑炎病毒、蜱传脑炎病毒、艾滋病病毒或丙型肝炎病毒，也能通过这些方法特异性地检测出登革热病毒。
• Methods of treating heart failure with agonists of hypocretin receptor 2
  申请人：The Board of Trustees of the Leland Stanford Junior University

  公开号：US10172916B2

  公开（公告）日：2019-01-08

  Methods of treating individuals for heart failure are disclosed. In particular, the invention relates to methods of treating individuals for heart failure with agonists of hypocretin receptor 2. The invention further relates to the use of genetic analysis of the single nucleotide polymorphism rs7767652, which resides immediately upstream of the HCRTR2 gene, to identify individuals in need of treatment for heart failure who are predicted to be more responsive to medical intervention.

  本发明公开了治疗心力衰竭患者的方法。特别是，本发明涉及使用视网膜下素受体 2 的激动剂治疗心力衰竭患者的方法。本发明还涉及利用紧邻 HCRTR2 基因上游的单核苷酸多态性 rs7767652 的遗传分析，来识别需要治疗心衰的个体，这些个体预计对医疗干预的反应更强。