(2Z)-2-[amino(hydroxy)methylidene]-4-(dimethylamino)-11,12a-dihydroxy-6-methyl-4a,5-dihydro-4H-tetracene-1,3,12-trione;hydrochloride

• 分子结构分类: 有机化合物 - 苯类化合物 - 萘
• 英文名称: (2Z)-2-[amino(hydroxy)methylidene]-4-(dimethylamino)-11,12a-dihydroxy-6-methyl-4a,5-dihydro-4H-tetracene-1,3,12-trione;hydrochloride
• 化学式: C22H23ClN2O6
• 分子量: 446.9
• InChiKey: FFZXKJVSZDKEMY-XGRJIHFXSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.17
• 重原子数：
  31
• 可旋转键数：
  1
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.32
• 拓扑面积：
  141
• 氢给体数：
  5
• 氢受体数：
  8

文献信息

• METHODS AND SYSTEMS FOR QUANTIFYING GENE EXPRESSION IN SYNTHETIC GENE CIRCUITS AND FOR TUNING SYNTHETIC GENE CIRUITS
  申请人：ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

  公开号：US20190221281A1

  公开（公告）日：2019-07-18

  A method of tuning the performance of a synthetic gene circuit comprising a plurality of genes is disclosed. The method includes quantifying, for each gene, a relative gene expression metric. The metric is quantified by determining a number of nucleotides in a 5′ adjacent transcriptional region (ATR) of the gene, a number of nucleotides in a 3′ ATR, a minimum free energy of a mRNA secondary structure around a ribosome binding site of the gene, a total folding energy for the 5′ ATR, and a folding energy for the first 100 nucleotides of the 3′ ATR, and then summing sequence-dependent energy changes, the terms including scaling coefficients and an average energy cost for synthesizing a nucleotide. The method further includes simulating, through application of the relative gene expression metrics of the plurality of genes to a deterministic model, the performance of an intended function of the synthetic gene circuit.
• [EN] REVERSE TRANSCRIPTION OF POLYNUCLEOTIDES COMPRISING UNNATURAL NUCLEOTIDES<br/>[FR] TRANSCRIPTION INVERSE DE POLYNUCLÉOTIDES COMPRENANT DES NUCLÉOTIDES NON NATURELS
  申请人：[en]THE SCRIPPS RESEARCH INSTITUTE

  公开号：WO2022087475A1

  公开（公告）日：2022-04-28

  Disclosed herein are methods of reverse transcribing a polynucleotide comprising an unnatural ribonucleotide comprising reverse transcribing the polynucleotide with a reverse transcriptase in the presence of an unnatural dNTP comprising an unnatural nucleobase, wherein the reverse transcriptase polymerizes cDNA into which the unnatural NTP is incorporated. In some embodiments, the polynucleotide is present at a concentration less than or equal to about 500 nM and/or the polynucleotide is a tRNA, mRNA, RNA ap tamer, or a member of a plurality of RNA aptamer candidates.