Spontaneous Membrane Fusion Induced by Chemical Formation of Ceramides in a Lipid Bilayer
摘要:
Mere chemical generation of ceramide and related double-chain lipids in the membrane of small unilamellar vesicles (SUVs) induces fusion of the vesicles. The lipids can be successfully prepared by dehydrocondensation between single-chain lipids (fatty acids and sphingosine or its analogues) in a lipid bilayer of the SUV by using a combination of 2-chloro-4,6-dimethoxy-1,3,5-triazine and amphiphilic tertiary amine catalysts, a process that can be compared to a successive enzyme model system for a fatty acyl-CoA synthetase followed by acyltransferase. The SUV spontaneously undergoes membrane fusion upon this internal chemical stimulation by the artificial enzyme system.
A neutral/alkaline ceramidase derived from a mammal; an antibody specifically binding thereto; a probe and primer which are capable of specifically hybridizing thereto; a method for producing the ceramidase by a genetic engineering means; a method for detecting the ceramidase or the gene; and a method of controlling an amount of a ceramide in a cell and/or in a tissue. The present invention is useful as a reagent for lipid engineering for analyzing a structure, functions, and the like of a ceramide, and in its applications to diseases associated with the ceramide metabolism.
ARTIFICIALLY SYNTHESIZED SPHINGOSINE DERIVATIVE LIPOID MONOMER AND USE OF SAME FOR DELIVERING NUCLEIC ACID
申请人:Institute of Basic Medical Sciences,
Chinese Academy of Medical Sciences
公开号:EP3777891A1
公开(公告)日:2021-02-17
Provided are an artificially synthesized sphingosine lipoid monomer and the use of same for delivering a nucleic acid. In particular, provided is the use or a method of using a compound of formula I, a stereoisomer thereof or a pharmaceutically acceptable salt thereof, or a combination comprising the compound of formula I, the stereoisomer thereof or the pharmaceutically acceptable salt thereof to deliver a nucleic acid to a cell or subject, (I)
本发明提供了一种人工合成的鞘氨醇类脂质单体及其用于递送核酸的用途。特别是,提供了使用式 I 的化合物、其立体异构体或其药学上可接受的盐,或包含式 I 的化合物、其立体异构体或其药学上可接受的盐的组合物向细胞或受试者递送核酸的用途或方法,(I)
AZIDO COMPOUNDS AND METHOD FOR MONITORING ACID CERAMIDASE ACTIVITY IN INTACT CELLS USING THEM
申请人:Consejo Superior de Investigaciones Científicas
(CSIC)
公开号:EP3925949A1
公开(公告)日:2021-12-22
The application relates to azido compounds of formula (I) and a method of using them together with a fluorogenic reporter as a click agent for the specific determination of acid ceramidase activity in intact cells. The application also relates to a kit to carry out said method and the use thereof for the diagnosis of a disease caused by the deregulation of ceramidase activity selected from Farber disease and cancer.
Various embodiments disclosed relate to drug-coated balloon catheters for treating strictures in body lumens and methods of using the same. A drug-coated balloon catheter for delivering a therapeutic agent to a target site of a body lumen stricture includes an elongated balloon having a main diameter. The balloon catheter includes a coating layer overlying an exterior surface of the balloon. The coating layer includes one or more water-soluble additives and an initial drug load of a therapeutic agent.
Various embodiments disclosed relate to drug-coated balloon catheters for treating strictures in body lumens and methods of using the same. A drug-coated balloon catheter for delivering a therapeutic agent to a target site of a body lumen stricture includes an elongated balloon having a main diameter. The balloon catheter includes a coating layer overlying an exterior surface of the balloon. The coating layer includes one or more water-soluble additives and an initial drug load of a therapeutic agent.