5-((3aR,4R,6aS)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(3,6,9,12-tetraoxapentadec-14-yn-1-yl)pentanamide

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 生物素及其衍生物
• 英文名称: 5-((3aR,4R,6aS)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(3,6,9,12-tetraoxapentadec-14-yn-1-yl)pentanamide
• 英文别名: 5-[(3aR,4R,6aS)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[2-[2-[2-(2-prop-2-ynoxyethoxy)ethoxy]ethoxy]ethyl]pentanamide
• 化学式: C₂₁H₃₅N₃O₆S
• 分子量: 457.591
• InChiKey: SKMJWNZZFUDLKQ-QWFCFKBJSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.4
• 重原子数：
  31
• 可旋转键数：
  18
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.81
• 拓扑面积：
  132
• 氢给体数：
  3
• 氢受体数：
  7

反应信息

• 作为反应物：
  描述：

  5-((3aR,4R,6aS)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(3,6,9,12-tetraoxapentadec-14-yn-1-yl)pentanamide 、 在 copper(II) sulfate 、 sodium ascorbate 、 三(3-羟丙基三唑甲基)胺 作用下， 以 水 为溶剂， 反应 16.0h， 生成
  参考文献：
  名称：

  Chemoenzymatic Assembly of Bacterial Glycoconjugates for Site-Specific Orthogonal Labeling

  摘要：

  The cell surfaces of bacteria are replete with diverse glycoconjugates that play pivotal roles in determining how bacteria interact with the environment and the hosts that they colonize. Studies to advance our understanding of these interactions rely on the availability of chemically defined glycoconjugates that can be selectively modified under orthogonal reaction conditions to serve as discrete ligands to probe biological interactions, in displayed arrays and as imaging agents. Herein, enzymes in the N-linked protein glycosylation (Pgl) pathway of Campylobacter jejuni are evaluated for their tolerance for azide-modified UDP-sugar substrates, including derivatives of 2,4-diacetamidobacillosamine and N-acetylgalactosamine. In vitro analyses reveal that chemoenzymatic approaches are useful for the preparation of undecaprenol diphosphate-linked glycans and glycopeptides with site-specific introduction of azide functionality for orthogonal labeling at three specific sites in the heptasaccharide glycan. The uniquely modified glycoconjugates represent valuable tools for investigating the roles of C. jejuni cell surface glycoconjugates in host pathogen interactions.

  DOI：

  10.1021/jacs.5b07146

文献信息

• Immunoassay for determination of trilobolide
  作者：Lukáš Huml、Michal Jurášek、Petra Mikšátková、Tomáš Zimmermann、Pavla Tomanová、Miloš Buděšínský、Zdeňka Rottnerová、Markéta Šimková、Juraj Harmatha、Eva Kmoníčková、Oldřich Lapčík、Pavel B. Drašar

  DOI：10.1016/j.steroids.2016.08.019

  日期：2017.1

  were obtained by immunization of rabbits.Indirect competitive ELISA for determination of trilobolide was developed.Immunoassay was used to quantify trilobolide in different parts of Laser trilobum. Abstract Trilobolide (Tb) is a pharmacologically interesting sesquiterpene lactone isolated from Laser trilobum (L.) Borkh. Structural relation to a sarco/endoplasmic reticulum Ca2+‐ATPase inhibitor thapsigargin

  图形抽象图。没有可用的字幕。亮点制备三叶内酯与牛血清白蛋白和生物素的新型偶联物。通过免疫家兔获得三叶内酯特异性多克隆抗体。开发了测定三叶内酯的间接竞争ELISA方法。采用免疫测定法对激光三叶内不同部位的三叶内酯进行定量。摘要 三叶内酯 (Tb) 是一种从 Laser trilobum (L.) Borkh 中分离出来的具有药理学意义的倍半萜内酯。与肌/内质网 Ca2+-ATPase 抑制剂毒胡萝卜素的结构关系为 Tb 用于开发新的抗癌药物带来了广阔的前景。只要有关其调查的问题仍未得到解答，就会出现对新型分析工具的需求。由于免疫测定是天然产物研究中的有力工具之一，因此描述了利用基于亲和素-生物素技术的涂层的间接竞争性酶联免疫吸附测定 (ELISA) 的开发。在我们的 ELISA 设置中，新合成的生物素化 Tb 作为固定竞争者。Tb-羧甲基肟-牛血清白蛋白 (BSA) 和 Tb-琥珀酰-BSA