Synthesis and Characterization of Layered Double Hydroxides and Their Potential as Nonviral Gene Delivery Vehicles
作者:Blake Balcomb、Moganavelli Singh、Sooboo Singh
DOI:10.1002/open.201402074
日期:2015.4
Layered double hydroxides (LDHs) exhibit characteristic anion‐exchange chemistry making them ideal carriers of negatively charged molecules like deoxyribonucleic acid (DNA). In this study, hydrotalcite (Mg−Al) and hydrotalcite‐like compounds (Mg−Fe, Zn−Al, and Zn−Fe), also known as LDHs, were evaluated for their potential application as a carrier of DNA. LDHs were prepared by coprecipitation at low
层状双氢氧化物(LDHs)具有独特的阴离子交换化学性质,使其成为带有负电荷的分子(如脱氧核糖核酸(DNA))的理想载体。在这项研究中,对水滑石(Mg-Al)和类水滑石化合物(Mg-Fe,Zn-Al和Zn-Fe)(也称为LDHs)进行了评估,将其作为DNA载体的潜在应用。LDH是通过低过饱和度的共沉淀制备的,并通过粉末X射线衍射(XRD),红外(IR),拉曼光谱和电感耦合等离子体发射光谱,扫描电子显微镜(SEM)和透射电子显微镜(TEM)进行表征。XRD图谱在(003)和(006)平面上显示出强烈而尖锐的衍射峰,表明晶态材料有序。TEM图像产生大小为50–250 nm的不规则圆形至六边形颗粒。对于所有化合物,观察到不同程度的DNA结合,核酸酶消化研究表明LDH对结合的DNA提供一定程度的保护。在人类胚胎肾(HEK293),子宫颈癌(HeLa)和肝细胞癌(HepG2)细胞系中观察到最小的毒性,