glutamate dehydrogenase (NADP+) | 9029-11-2

• 物质功能分类: 生物化工 - 氨基酸及其衍生物 - 谷氨酸类衍生物
• 分子结构分类: 氧化还原酶 - 作用于CH-NH2供体组
• 英文名称: glutamate dehydrogenase (NADP+)
• 英文别名: glutamic dehydrogenase；dehydrogenase, glutamate (nicotinamide adenine dinucleotide (phosphate))；glutamic acid dehydrogenase；L-glutamate dehydrogenase；L-glutamic acid dehydrogenase；NAD(P)+-glutamate dehydrogenase；NAD(P)H-dependent glutamate dehydrogenase；L-glutamic dehydrogenase
• CAS: 9029-11-2

安全信息

• 安全说明：
  S24/25
• WGK Germany：
  3
• 海关编码：
  35079090

反应信息

• 作为试剂：
  描述：

  β-烟酰胺腺嘌呤二核苷酸 在 formate dehydrogenase 、 甲酸-d2 、 glutamate dehydrogenase (NADP+) 、 2-氧代-戊二酸离子(2-) 、 sodium carbonate 、 氯化铵 作用下， 以 水 为溶剂， 反应 3.0h， 生成 [4R,S-2H2]-NADH
  参考文献：
  名称：

  Chemical formation of 4-hydroxy-2,5-dimethyl-3[2H]-furanone from d-fructose 1,6-diphosphate

  摘要：

  The selective chemical formation of 4-hydroxy-2,5-dimethyl-3[2H]-furanone (HDF) from D-fructose 1,6-diphosphate in the presence of reduced nicotinamide-adenine-dinucleotides (NAD(P)H) was investigated by means of HPLC-DAD and HPLC-UV-MS/MS. The temperature optimum for HDF formation was 30 degreesC, whereas the pH value (pH 3-10) and chemical nature of the buffer had no significant influence. A linear correlation of reaction time and D-fructose 1,6-diphosphate concentration with the obtained HDF yield was observed. Proteins appeared to have a stabilizing effect. The NAD(P)H were mandatory, even in the presence of protein, implying a non-enzymatic hydride-transfer to an unknown intermediate which finally leads to the selective formation of HDF. The hydride-transfer was confirmed by the application of selectively pro-4R or pro-4S deuterium labeled NADH resulting in each case in the formation of HDF exhibiting a deuterium labeling of approx 30% and employment of [4R,S-H-2(2)]-NADH led to a deuterium labeling of approx 66%. The incubation of [1-C-13]-D-fructose 1,6-diphosphate with [4R,S-H-2(2)]-NADH revealed that the hydride is transferred to C-5 or C-6 of the D-fructose 1,6-diphosphate skeleton. Thus, a chemical HDF formation from D-fructose 1,6-diphosphate under physiological reaction conditions was shown and for the first time to our knowledge a non-enzymatic hydride-transfer from NADH to a carbohydrate structure was demonstrated. (C) 2002 Elsevier Science Ltd. All rights reserved.

  DOI：

  10.1016/s0008-6215(02)00121-0