3',6'-二羟基-4',5'-二甲氧基-1-氧代螺[2-苯并呋喃-3,9'-氧杂蒽]-5-羧酸 | 75929-58-7

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 中文名称: 3',6'-二羟基-4',5'-二甲氧基-1-氧代螺[2-苯并呋喃-3,9'-氧杂蒽]-5-羧酸
• 英文名称: 4',5'-Dimethoxy-6-carboxyfluorescein
• 英文别名: 3',6'-dihydroxy-4',5'-dimethoxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylic acid
• CAS: 75929-58-7
• 化学式: C₂₃H₁₆O₉
• 分子量: 436.4
• InChiKey: DCPHSBOTFHCNQC-UHFFFAOYSA-N

物化性质

• 沸点：
  774.9±60.0 °C(Predicted)
• 密度：
  1.67±0.1 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  2.9
• 重原子数：
  32
• 可旋转键数：
  3
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.13
• 拓扑面积：
  132
• 氢给体数：
  3
• 氢受体数：
  9

文献信息

• Novel ether substituted fluorescein compounds as fluorescers and quenchers
  申请人：SYVA COMPANY

  公开号：EP0050684A1

  公开（公告）日：1982-05-05

  Diether symmetrically substituted fluoresceins are provided having at least one anionic group and a linking functionality. Depending upon the site of substitution, the compounds can be used as fluorescers absorbing at wavelengths in excess of 500nm or as quenchers, absorbing at wavelengths in excess of 500 nm and exhibiting substantially no fluorescence. The compounds find wide application. particularly as labels in fluorescent immunoassays.

  二醚对称取代荧光素具有至少一个阴离子基团和一个连接官能团。根据取代部位的不同，这些化合物可以用作吸收波长超过 500 纳米的荧光剂，也可以用作淬灭剂，吸收波长超过 500 纳米，但基本上不产生荧光。这些化合物应用广泛，特别是作为荧光免疫分析中的标签。
• Hybridization assay employing labeled pairs of hybrid binding reagents
  申请人：MILES INC.

  公开号：EP0144914A2

  公开（公告）日：1985-06-19

  @ A nucleic acid hybridization assay wherein the hybrid formed with the probe has binding sites for two specific binding reagents, one of which comprises a first label and the other a second label, interaction of the first and second labels providing a detectable response which is measurably different when the two labeled reagents are both bound to the same hybrid compared to when the two labeled reagents are not so bound. The formation of the hybrid assay product brings the two labels within a proximate interaction distance of one another, e.g., as in the cases of sequential catalyst (enzyme) interaction and energy transfer. Since the labels provide a response which is distinguishable when they are associated with a hybridized probe, no separation step is required.

  一种核酸杂交检测方法，其中与探针形成的杂交体具有两个特异性结合试剂的结合位点，其中一个包括第一标签，另一个包括第二标签，第一标签和第二标签的相互作用提供了可检测的反应，当两个标签试剂都结合到同一个杂交体上时，与两个标签试剂没有结合到同一个杂交体上时相比，反应有可测量的差异。 杂交检测产物的形成使两个标签在彼此近似的相互作用距离内，例如，在顺序催化剂（酶）相互作用和能量转移的情况下。 由于标签在与杂交探针结合时提供了可区分的反应，因此不需要分离步骤。
• Hybridization assay employing labeled probe and anti-hybrid
  申请人：MILES INC.

  公开号：EP0144913A2

  公开（公告）日：1985-06-19

  A nucleic acid hybridization assay involving a labeled probe and formation of a hybrid having epitopes for an antibody reagent. The label provides a detectable response which is measurably different when the labeled probe is comprised in a hybrid that is bound by the antibody reagent compared to when not comprised in such a hybrid. Particularly useful antibody reagents are antibodies such as anti-DNA'RNA, anti-RNA'RNA and antibodies to intercalated duplexes which do not bind substantially to single stranded nucleic acids. Modulation of the label response can be accomplished in a variety of ways such as by steric inactivation or hindrance or by labeling the antibody reagent with a second label which interacts with the first label. The assay is particularly advantageous because no separation of hybridized and unhybridized probe is required.

  一种核酸杂交试验，涉及标记探针和具有抗体试剂表位的杂交体的形成。当标记探针与抗体试剂结合的杂交体中含有标记探针时，与不含有标记探针的杂交体中含有标记探针时相比，标记探针会产生可测量的不同反应。特别有用的抗体试剂是抗 DNA'RNA、抗 RNA'RNA 和不与单链核酸结合的夹层双链抗体。标记反应的调节可以通过多种方式实现，如立体失活或阻碍，或用与第一个标记相互作用的第二个标记对抗体试剂进行标记。由于无需分离杂交探针和未杂交探针，因此这种检测方法特别有优势。
• Method of and kit for immunoassay
  申请人：Tosoh Corporation

  公开号：EP0476545A1

  公开（公告）日：1992-03-25

  Using a combination of a fine particle (A) having bound thereto a fluorescent substance and an antibody reacting specifically with a target substance to be detected, and a fine particle (B) having bound thereto a quencher and an antibody reacting specifically with the target substance, through a different antigen determinant, the sandwich immunoassay is conducted and a quenchng of the fluorescence is measured. Alternatively, a combination of a fine particle (C) having bound thereto one member selected from a fluorescent substance and a quencher, and a bound product (D) composed of the other member selected from the fluorescent substance and the quencher, and a known amount of the target substance is used.

  通过不同的抗原决定因子，使用结合了荧光物质和与待检测目标物质特异性反应的抗体的细颗粒（A），以及结合了淬灭剂和与目标物质特异性反应的抗体的细颗粒（B）的组合，进行夹心免疫测定，并测量荧光的淬灭。另一种方法是使用一种细颗粒（C），其上结合有从荧光物质和淬灭剂中选出的一种成分，以及由从荧光物质和淬灭剂中选出的另一种成分和已知量的目标物质组成的结合产物（D）。
• Digital immunoassay
  申请人：Bio-Rad Laboratories, Inc.

  公开号：US10809250B2

  公开（公告）日：2020-10-20

  Methods of detecting an analyte in a sample are provided. In one embodiment, the method comprises forming a partition comprising the sample, a binding agent capable of emitting a signal when bound to the analyte, and a marker capable of identifying the partition; allowing the binding agent to bind to the analyte, if present; and detecting the presence of the analyte in the sample by detecting the signal emitted from the binding agent in the partition.

  本文提供了检测样品中分析物的方法。在一个实施方案中，该方法包括形成一个分区，该分区包括样品、与被分析物结合时能够发出信号的结合剂以及能够识别该分区的标记；允许结合剂与被分析物结合（如果存在的话）；以及通过检测分区中结合剂发出的信号来检测样品中是否存在被分析物。