[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-hydroxyphosphoryl]oxy-N-[[4-[3-(trifluoromethyl)diazirin-3-yl]phenyl]methyl]phosphonamidic acid | 1305036-87-6

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘌呤核苷酸
• 英文名称: [[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-hydroxyphosphoryl]oxy-N-[[4-[3-(trifluoromethyl)diazirin-3-yl]phenyl]methyl]phosphonamidic acid
• CAS: 1305036-87-6
• 化学式: C₁₉H₂₂F₃N₈O₁₂P₃
• 分子量: 704.347
• InChiKey: HCXFCBAXJIPKPE-LSCFUAHRSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -3
• 重原子数：
  45
• 可旋转键数：
  12
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.42
• 拓扑面积：
  296
• 氢给体数：
  7
• 氢受体数：
  22

反应信息

• 作为产物：
  描述：

  2-[4-(3-Trifluoromethyl-3H-diazirin-3-yl)-benzyl]-isoindole-1,3-dione 在 盐酸-N-乙基-Nˊ-(3-二甲氨基丙基)碳二亚胺 、 甲胺 作用下， 以 甲醇 为溶剂， 生成 [[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-hydroxyphosphoryl]oxy-N-[[4-[3-(trifluoromethyl)diazirin-3-yl]phenyl]methyl]phosphonamidic acid
  参考文献：
  名称：

  Rapidly photoactivatable ATP probes for specific labeling of tropomyosin within the actomyosin protein complex

  摘要：

  Tropomyosin-specific photoaffinity adenosine triphosphate (ATP) probes have been first developed, in which a diazirine moiety is incorporated into the gamma-phosphate group as a rapidly carbene-generating photophore. These probes clearly labeled tropomyosin in the presence of other actomyosin components, that is, myosin, actin, and troponins. The specific labeling of tropomyosin was easily identified by selective trapping of the photo-incorporated ATP probe on Fe3+-immobilized metal ion affinity chromatography (IMAC) beads. The characteristic nature of tropomyosin-specific photocross-linking was further confirmed with a biotin-carrying derivative of the ATP probe. These data suggest that the tropomyosin on the actin filament assembly is located in close proximity to the ATP binding cavity of myosin. (C) 2011 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmcl.2011.02.113

文献信息

• Rapidly photoactivatable ATP probes for specific labeling of tropomyosin within the actomyosin protein complex
  作者：Souta Masuda、Takenori Tomohiro、Yasumaru Hatanaka

  DOI：10.1016/j.bmcl.2011.02.113

  日期：2011.4

  Tropomyosin-specific photoaffinity adenosine triphosphate (ATP) probes have been first developed, in which a diazirine moiety is incorporated into the gamma-phosphate group as a rapidly carbene-generating photophore. These probes clearly labeled tropomyosin in the presence of other actomyosin components, that is, myosin, actin, and troponins. The specific labeling of tropomyosin was easily identified by selective trapping of the photo-incorporated ATP probe on Fe3+-immobilized metal ion affinity chromatography (IMAC) beads. The characteristic nature of tropomyosin-specific photocross-linking was further confirmed with a biotin-carrying derivative of the ATP probe. These data suggest that the tropomyosin on the actin filament assembly is located in close proximity to the ATP binding cavity of myosin. (C) 2011 Elsevier Ltd. All rights reserved.