| 1092458-70-2

• 分子结构分类: 有机化合物 - 苯类化合物 - 萘
• CAS: 1092458-70-2
• 化学式: C₁₈H₂₉N₅O₂S*ClH
• 分子量: 415.987
• InChiKey: JGSGZJZDKJSADN-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.83
• 重原子数：
  27.0
• 可旋转键数：
  10.0
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.44
• 拓扑面积：
  104.69
• 氢给体数：
  3.0
• 氢受体数：
  6.0

反应信息

• 作为产物：
  描述：

  N-{2-[bis(2-aminoethyl)amino]ethyl}-5-(dimethylamino)naphthalene-1-sulfonamide 在 盐酸 作用下， 以 二氯甲烷 、 水 为溶剂， 反应 12.0h， 以1.00 g的产率得到
  参考文献：
  名称：

  Synthesis and Characterization of Fluorescent Displacers for Online Monitoring of Displacement Chromatography

  摘要：

  One of the major impediments to the implementation of displacement chromatography for the purification of biomolecules is the need to collect fractions from the column effluent for time-consuming off line analysis. The ability to employ direct online monitoring of displacement chromatography would have significant implications for applications ranging from analytical to preparative bioseparations. To this end, a set of novel fluorescent displacers were rationally designed using known chemically selective displacers as a template. Fluorescent cores were functionalized with different charge moieties, creating a homologous library of displacers. These compounds were then tested on two protein pairs, alpha-chymotrypsinogen A/ribonuclease A and cytochrome c/lysozyme, using batch and column displacement experiments. Of the synthesized displacers, two were found to be highly selective while one was determined to be a high-affinity displacer. Column displacements using one of the selective displacers yielded complete separation of both protein pairs while facilitating direct online detection using UV and fluorescence detection. Saturation transfer difference NMR was also carried out to investigate the binding of the fluorescent displacers to proteins. The results indicated a selective binding between the selective displacers and (x-chymotrypsinogen A, while no binding was observed for ribonuclease A, confirming that protein-displacer binding is responsible for the selectivity in these systems. This work demonstrates the utility of fluorescent displacers to enable online monitoring of displacer breakthroughs while also acting as efficient displacers for protein purification.

  DOI：

  10.1021/ja806279x

文献信息

• Dansylated Polyamines as Fluorescent Sensors for Metal Ions: Photophysical Properties and Stability of Copper(II) Complexes in Solution
  作者：Luca Prodi、Marco Montalti、Nelsi Zaccheroni、Francesco Dallavalle、Guiseppina Folesani、Maurizio Lanfranchi、Roberto Corradini、Sara Pagliari、Rosangela Marchelli

  DOI：10.1002/1522-2675(20010321)84:3<690::aid-hlca690>3.0.co;2-l

  日期：2001.3.21

  The complexes formed by these ligands in neutral form correspond to those observed for the analogous unsubstituted monoprotonated amines, whereas, when the ligands are deprotonated at the sulfonamide moiety, the species parallel those of the corresponding amines. The molecular structures of the complexes were deduced from the VIS absorption spectra. The crystal structure of the [CuL2H−2] complex 6 of

  丹磺酰基化多胺 N-丹磺酰基乙二胺 (1)、N-丹磺酰基二乙烯三胺 (2)、N-丹磺酰基三乙烯四胺 (3)、N'-[2-(丹磺酰基氨基)乙基]二乙烯三胺 (4) 和三(4) 的质子化和 CuII 络合常数2-丹磺酰基氨基乙基)胺(5)通过玻璃电极电位法在MeOH/H2O 9:1 (v/v)溶液中测定。对于配体 3 和 4，测定也在水溶液中进行。由这些配体以中性形式形成的配合物对应于对类似的未取代的单质子化胺所观察到的配合物，而当配体在磺酰胺部分去质子化时，这些物质与相应胺的物质平行。配合物的分子结构由可见吸收光谱推断。配体 1 (L) 的 [CuL2H-2] 配合物 6 的晶体结构通过 X 射线衍射确定。对配体 3 – 5 的光物理性质的研究表明，它们是铜 (II) 的良好荧光传感器，可诱导荧光猝灭。时间分辨荧光测量使我们能够阐明传感机制。淬灭效应的 pH 依赖性表明，它发生在所有 Cu2+