3,5-diphenyl-4,5-dihydro-1H-pyrazole-4-carbonitrile | 101117-38-8

• 分子结构分类: 有机化合物 - 苯丙烷和聚酮 - 二苯乙烯类
• 英文名称: 3,5-diphenyl-4,5-dihydro-1H-pyrazole-4-carbonitrile
• 英文别名: 3,5-Diphenyl-4,5-dihydro-1H-pyrazol-4-carbonitril
• CAS: 101117-38-8
• 化学式: C₁₆H₁₃N₃
• 分子量: 247.299
• InChiKey: JMJKYKBTSIGATM-UHFFFAOYSA-N

反应信息

• 作为产物：
  描述：

  alkaline earth salt of/the/ methylsulfuric acid 在 盐酸肼 、 乙醇 作用下， 生成 3,5-diphenyl-4,5-dihydro-1H-pyrazole-4-carbonitrile
  参考文献：
  名称：

  Analysis of Buprenorphine, Norbuprenorphine, and Their Glucuronides in Urine by Liquid Chromatography-Mass Spectrometry

  摘要：

  Buprenorphine is used for the treatment of chronic pain and also in treatment of heroin addiction as an alternative to methadone. As the availability of buprenorphine increases, so does the risk for abuse and the pressure on forensic and clinical laboratories to analyze for it. Buprenorphine and its dealkylated metabolite are excreted in urine, almost exclusively as glucuronides. The aim of the present study was to evaluate electrospray liquid chromatography tandem mass spectrometry (LC-MS-MS) for the rapid screening and quantitation of buprenorphine and its metabolites in urine. Three approaches were evaluated: (1) direct injection of diluted urine for measurement of glucuronides, (2) direct injection of diluted urine after enzymatic hydrolysis for the quantitation of buprenorphine and norbuprenorphine, and (3) quantitation of buprenorphine and norbuprenorphine after enzymatic hydrolysis and solid-phase extraction (SPE). One hundred six samples were subjected to procedure 1 and, when positive, further quantitated using procedure 2. Only samples with low analyle concentrations (< 20 µg/L) were subject to SPE. Concentrations of buprenorphine and norbuprenorphine in patients (N = 16) ranged between 31 and 1080 µg/L and 48–2050 µg/L, respectively. In suspected abusers (N = 33), the ranges were 2.3–796 µg/L and 5.0–2580 µg/L. In four of the authentic samples, both the buprenorphine and norbuprenorphine concentrations were below the 20-µg/L cutoff. We concluded that LC-MS-MS analysis of the glucuronides provided an adequate screening method, but that the direct method for quantitation sometimes had to be complemented with a concentration by SPE, providing increased sensitivity, thus lowering the cutoff from 20 to 1 µg/L urine.

  DOI：

  10.1093/jat/27.7.464