The present teachings provide for composite substrates for the covalent attachment of biomolecules and method of making the same. The present teachings provide for composite substrates comprising a porous copolymer-monolith covalently attached to a surface of a substrate, wherein the porous copolymer-monolith has been formed by an inverse phase photo-copolymerization process comprising photo-copolymerizing at least one ethylenically unsaturated monomer with polymerizable surface functionalities that are covalently attached to a surface of a derivitized substrate such that, after photo-copolymerization, the porous copolymer-monolith is covalently attached to the surface of the substrate, and wherein the photo-copolymerizing is carried out in the presence of at least one porogenic solvent.
本发明提供了用于共价连接
生物分子的复合基底及其制造方法。本发明提供的复合基底包括共价连接到基底表面的多孔共聚单体,其中多孔共聚单体是通过反相光聚合工艺形成的,该工艺包括至少一种具有可聚合表面官能团的
乙烯基不饱和单体的光聚合,该单体共价连接到衍生化基底的表面,从而: 光聚合后,多孔共聚单体共价连接到基底的表面、光聚合后,多孔共聚单体共价连接到基底表面,其中光聚合是在至少一种致孔溶剂存在下进行的。