4-hydroxy-3-((2-hydroxy-5-nitro-3-sulfophenyl)diazenyl)naphthalene-1-sulfonic acid | 24801-50-1

• 分子结构分类: 有机化合物 - 苯类化合物 - 萘
• 英文名称: 4-hydroxy-3-((2-hydroxy-5-nitro-3-sulfophenyl)diazenyl)naphthalene-1-sulfonic acid
• 英文别名: 2,4-Sulfonitrophenol NV；3-[2-(2-Hydroxy-5-nitro-3-sulfophenyl)hydrazinylidene]-4-oxo-3,4-dihydronaphthalene-1-sulfonic acid；4-hydroxy-3-[(2-hydroxy-5-nitro-3-sulfophenyl)diazenyl]naphthalene-1-sulfonic acid
• CAS: 24801-50-1
• 化学式: C₁₆H₁₁N₃O₁₀S₂
• 分子量: 469.409
• InChiKey: HAULYPRFJOJLKA-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2.1
• 重原子数：
  31
• 可旋转键数：
  4
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.0
• 拓扑面积：
  237
• 氢给体数：
  4
• 氢受体数：
  12

反应信息

• 作为产物：
  描述：

  1-萘酚-4-磺酸 、 在 sodium hydroxide 作用下， 以 水 为溶剂， 反应 2.0h， 以38%的产率得到4-hydroxy-3-((2-hydroxy-5-nitro-3-sulfophenyl)diazenyl)naphthalene-1-sulfonic acid
  参考文献：
  名称：

  Development of MRSA and MRAB detection kit employing loop–mediated isothermal amplification and colorimetric indicator dye

  摘要：

  Methicillin-resistant Staphylococcus aureus (MRSA) and Multidrug-resistant Acinetobacter baumannii (MRAB) are serious life-threating pathogens. The rapid detection of those pathogens is a challenge for the most clinical microbiology laboratories. A nucleic acid amplification technique, loop-mediated isothermal amplification (LAMP) that enables to amplify target DNA sequences in a short time period, is an excellent technique to detect the pathogens with high efficiency. We developed LAMP detecting kits against both MRSA and MRAB that utilizing a newly developed colorimetric Mg2+ ion indicator dye D-649 and novel sets of primers. An aqueous soluble dye D-649 is stable over physiological pH range and displays clear color change upon binding to Mg2+ ion. The addition of 0.12 mM of D-649 to LAMP solution did not disturb the amplification process, and the positive reaction would be easily confirmed by the color change of reaction mixture from ruby to purple in naked eye under ambient light. Each primer set was designed to recognize mecA/fernB gene of MASA and blaOXA-23/blaOXA51 like gene of MRAB, and the detection lirnits for both DNAs were shown as 1 pg. These LAMP detection kits will be beneficial for the detection of MRSA and MRAB in clinical laboratories.

  DOI：

  10.1016/j.dyepig.2019.107618