Methods and reagents are provided for the rapid extraction of nucleic acids from a fixed paraffin embedded sample (e.g., a FFPET sample). In some embodiments, the methods comprise incubating one or more sections of said tissue sample in a lysis solution comprising a buffer sufficient to maintain the pH of said solution at a pH ranging from about pH 4 to about pH 9; a chaotropic agent; a chelating agent; and a detergent; where the incubating is at a temperature ranging from about 50°C to about 100°C; and recovering the nucleic acid from said lysis solution.
本文提供了从固定石蜡包埋样本(如 FFPET 样本)中快速提取核酸的方法和试剂。在一些实施方案中,这些方法包括将所述组织样本的一个或多个切片放入裂解液中孵育,裂解液包含足以将所述溶液的 pH 值维持在约 pH 4 至约 pH 9 之间的缓冲液;混沌剂;
螯合剂;以及去垢剂;其中孵育温度为约 50°C 至约 100°C;以及从所述裂解液中回收核酸。