fluorescein amine | 43043-76-1

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 英文名称: fluorescein amine
• 英文别名: Azane;3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
• CAS: 43043-76-1
• 化学式: C₂₀H₁₂O₅*H₃N
• 分子量: 349.343
• InChiKey: FBOXXXRZJFWBPD-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.41
• 重原子数：
  26
• 可旋转键数：
  0
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.05
• 拓扑面积：
  79.8
• 氢给体数：
  2
• 氢受体数：
  5

反应信息

• 作为反应物：
  描述：

  fluorescein amine 、 亚硝酸正戊酯 在 sodium chloride 、 硫酸 作用下， 以 N-甲基乙酰胺 、 水 为溶剂， 生成 azidofluorescein
  参考文献：
  名称：

  Derivatives of fluorescein

  摘要：

  这项发明涉及荧光素衍生物及其生产。这些化合物在确定细胞内pH值方面具有价值。因此，可以获得具有相对稳定荧光水平的荧光活细胞。这种新型荧光素化合物可用于生物学和生物化学中的其他标记目的。

  公开号：

  US04609740A1

文献信息

• ALKYL AMINE COMPOUNDS FOR FLUORESCENT LABELING
  申请人：Reed Michael W.

  公开号：US20120301880A1

  公开（公告）日：2012-11-29

  Alkyl amine dyes, oligonucleotide probes prepared from the alkyl amine dyes, phosphoramidites and solid supports prepared from the alkyl amine dyes, and methods of labeling biological agents using the alkyl amine dyes.

  烷基胺染料，由烷基胺染料制备的寡核苷酸探针，由烷基胺染料制备的磷酰胺酸酯和固相支持物，以及使用烷基胺染料标记生物试剂的方法。
• Methadone fluorescence polarization immunoassay
  申请人：Abbott Laboratories

  公开号：US05073629A1

  公开（公告）日：1991-12-17

  A fluorescence polarization immunoassay for detecting the presence of methadone in a test sample is provided, based upon competition between methadone and a fluorescently labeled tracer for the binding sites on an antibody specific for methadone. The concentration of methadone in the sample determines the amount of tracer that binds to the antibody. The amount of tracer-antibody complex formed can be quantitatively measured and is inversely proportional to the quantity of methadone in the sample. Tracers used as reagents and immunogens used to raise antibodies for use as reagents are also disclosed.

  提供了一种用于检测测试样本中甲庚酮存在的荧光偏振免疫分析方法，该方法基于甲庚酮与荧光标记示踪剂在特异于甲庚酮的抗体结合位点上的竞争。样本中甲庚酮的浓度决定了与抗体结合的示踪剂的数量。形成的示踪剂-抗体复合物的数量可以定量测量，并且与样本中甲庚酮的数量成反比。还公开了用作试剂的示踪剂和用于培养用作试剂的抗体的免疫原。
• Procainamide assay, tracers, immunogens and antibodies
  申请人：ABBOTT LABORATORIES

  公开号：EP0199042A1

  公开（公告）日：1986-10-29

  The present invention is directed to a fluorescence polarization immunoassay for procainamide, to the various components needed for preparing and carrying out such an assay, and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for making them. The tracers and the immunogens are made from analogs of procainamide. A fluorescein moiety is included in the tracer while a poly(amino acid) forms a part of the immunogen. The assay is conducted by measuring the degree of ' polarization retention of plane polarized light that has been passed through a sample containing antiserum and tracer.

  该发明涉及一种用于普鲁卡因胺的荧光偏振免疫分析，以及制备和执行此类分析所需的各种组分，以及制造这些组分的方法。具体地，揭示了示踪剂、免疫原和抗体，以及制造它们的方法。示踪剂和免疫原是由普鲁卡因胺的类似物制成的。示踪剂中包含荧光基团，而多肽（氨基酸）是免疫原的一部分。该分析通过测量通过含有抗血清和示踪剂的样品的偏振保留度来进行，该样品已经通过平面偏振光。
• [EN] NOVEL COMPOSITIONS FOR LABELING BIOMOLECULES AND METHODS USING SAME<br/>[FR] NOUVELLES COMPOSITIONS DE MARQUAGE DE BIOMOLÉCULES ET PROCÉDÉS LES UTILISANT
  申请人：UNIV CALIFORNIA

  公开号：WO2017196544A1

  公开（公告）日：2017-11-16

  The present invention includes novel compounds useful for labeling biomolecules. The present invention further includes a novel method of labeling a biomolecule using a compound of the invention. The present invention further includes a novel method of imaging a biomolecule using a compound of the invention.

  本发明包括用于标记生物分子的新化合物。本发明还包括使用该发明的化合物标记生物分子的新方法。本发明还包括使用该发明的化合物成像生物分子的新方法。
• Compositions and methods for coupling a plurality of compounds to a scaffold
  申请人：The Scripps Research Institute

  公开号：EP2452936A1

  公开（公告）日：2012-05-16

  Compositions and methods are provided for coupling a plurality of compounds to a scaffold. Compositions and methods are further provided for catalyzing a reaction between at least one terminal alkyne moiety and at least one azide moiety, wherein one moiety is attached to the compound and the other moiety is attached to the scaffold, forming at least one triazole thereby.

  本发明提供了将多种化合物耦合到支架上的组合物和方法。本发明还提供了在至少一个末端炔基团与至少一个叠氮基团之间催化反应的组合物和方法，其中一个基团附着在化合物上，另一个基团附着在支架上，从而形成至少一种三唑类化合物。