5-(氨基甲基)-3',6'-二羟基螺[2-苯并呋喃-3,9'-氧杂蒽]-1-酮 | 138588-54-2

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 中文名称: 5-(氨基甲基)-3',6'-二羟基螺[2-苯并呋喃-3,9'-氧杂蒽]-1-酮
• 中文别名: 二苯甲基(6R-trans)-7-氨基-3-甲氧基-8-羰基-5-硫杂-1-氮杂二环[4.2.0]辛-2-烯-2-羧酸酯盐酸盐
• 英文名称: 6-aminomethylfluorescein
• 英文别名: 6-(Aminomethyl)fluorescein；5-(aminomethyl)-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
• CAS: 138588-54-2
• 化学式: C₂₁H₁₅NO₅
• 分子量: 361.354
• InChiKey: QKEZGSCYUMTKFP-UHFFFAOYSA-N

物化性质

• 沸点：
  667.6±55.0 °C(Predicted)
• 密度：
  1.59±0.1 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  2.3
• 重原子数：
  27
• 可旋转键数：
  1
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.1
• 拓扑面积：
  102
• 氢给体数：
  3
• 氢受体数：
  6

文献信息

• Fluorescent probes for use in protein kinase inhibitor binding assay
  申请人：Prokopowicz S. Anthony

  公开号：US20050100978A1

  公开（公告）日：2005-05-12

  The invention provides methods relating to a novel screening assay format that can be applied to broad members of the protein kinase gene family. The assay uses a series of labeled, active site probes described herein that can be displaced by an inhibitor agent. The K d for the inhibitor compound is derived based on the K d of the probe for the kinase and the dose response of the inhibitor agent. The invention also provides novel active site probes suitable for use with the screening method.

  该发明提供了一种新型筛选检测方法，可应用于蛋白激酶基因家族的广泛成员。该检测方法使用一系列标记的活性位点探针，这些探针可以被抑制剂代替。抑制剂化合物的Kd是基于探针对激酶的Kd和抑制剂代理的剂量反应推导出来的。该发明还提供了适用于筛选方法的新型活性位点探针。
• Reagents and method for detecting polychlorinated biphenyls
  申请人：ABBOTT LABORATORIES

  公开号：EP0455058A2

  公开（公告）日：1991-11-06

  Reagents and an immunoassay for detecting the presence or amount of polychlorinated biphenyls in a test sample. The assay is performed by adding a known concentration of a tracer labeled with a detectable moiety and a known concentration of an analyte-specific antibody to a test sample to form a mixture, incubating the mixture to form labeled tracer-antibody and analyte-antibody complexes, and determining the presence or amount of tracer-antibody complexes formed as a measure of the presence or amount of analyte in the test sample. Reagents provided include tracers, immunogens and an additive compound useful in preventing non-specific binding of the polychlorinated biphenyls to proteins which may be present

  用于检测测试样品中是否存在多氯联苯或其含量的试剂和免疫测定法。检测方法是将已知浓度的标记有可检测分子的示踪剂和已知浓度的分析物特异性抗体添加到测试样品中形成混合物，孵育混合物以形成标记的示踪剂-抗体和分析物-抗体复合物，并确定所形成的示踪剂-抗体复合物的存在或数量，以此来衡量测试样品中分析物的存在或数量。所提供的试剂包括示踪剂、免疫原和一种添加剂化合物，可防止多氯联苯与可能存在的蛋白质发生非特异性结合。
• Immunoassay reagents and method for determining cyclosporine
  申请人：ABBOTT LABORATORIES

  公开号：EP0473961A2

  公开（公告）日：1992-03-11

  Cyclosporine derivatives useful as detectable tracer compounds for the immunoassay determination of cyclosporine are disclosed. The cyclosporine derivatives comprise a detectable moiety coupled to the amino acid at the first position (MeBmt) in cyclosporine, the second position (Abu) in cyclosporine, the third position (Sar) in cyclosporine, the eighth position (D-Ala) in cyclosporine, or the tenth position (MeLeu) in cyclosporine. A preferred cyclosporine derivative comprises a fluorescent moiety coupled to the hydroxyl group of the amino acid at the first position in cyclosporine, and is especially useful for the fluorescent polarization immunoassay deterimination of cyclosporine. A fluorescent polarization immunoassay method and test kit are also disclosed.

  本发明公开了可用作免疫测定环孢素的可检测示踪化合物的环孢素衍生物。这些环孢素衍生物包括与环孢素第一位（MeBmt）、环孢素第二位（Abu）、环孢素第三位（Sar）、环孢素第八位（D-Ala）或环孢素第十位（MeLeu）氨基酸偶联的可检测分子。一种优选的环孢素衍生物包含一个与环孢素中第一位氨基酸的羟基偶联的荧光分子，特别适用于荧光偏振免疫测定环孢素。还公开了一种荧光偏振免疫测定方法和检测试剂盒。
• Immunoassay for the detection of human autoantibodies
  申请人：ABBOTT LABORATORIES

  公开号：EP0651253A1

  公开（公告）日：1995-05-03

  An assay and test kit for the determination of LKM-1 autoantibodies in test samples suspected of containing anti-LKM-1 autoantibodies. The method uses a solid phase which preferably is a microparticle. The method is standardized and can be performed in automated systems, allowing quantitation of the amount of anti-LKM antibody in test samples.

  一种测定疑似含有抗 LKM-1 自身抗体的检测样本中 LKM-1 自身抗体的检测方法和检测试剂盒。该方法使用的固相最好是微粒。该方法标准化，可在自动化系统中进行，可定量检测样本中的抗 LKM 抗体含量。
• High molecular weight polysaccharide that binds and inhibits virus
  申请人：World Force Technologies, LLC

  公开号：US10232007B2

  公开（公告）日：2019-03-19

  This invention provides a high molecular weight polysaccharide capable of binding to and inhibiting virus and related pharmaceutical formulations and methods of inhibiting viral infectivity and/or pathogenicity, as well as immunogenic compositions. The invention further includes methods of inhibiting the growth of cancer cells and of ameliorating a symptom of aging. Additionally, the invention provides methods of detecting and/or quantifying and/or isolating viruses.

  本发明提供了一种能够与病毒结合并抑制病毒的高分子量多糖，以及抑制病毒传染性和/或致病性的相关药物制剂和方法，以及免疫原性组合物。本发明还包括抑制癌细胞生长和改善衰老症状的方法。此外，本发明还提供了检测和/或量化和/或分离病毒的方法。