9-(2-Carboxy-4-isothiocyanato-phenyl)-3,6-bis-dimethylamino-xanthenylium

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 英文名称: 9-(2-Carboxy-4-isothiocyanato-phenyl)-3,6-bis-dimethylamino-xanthenylium
• 英文别名: [9-(2-Carboxy-4-isothiocyanatophenyl)-6-(dimethylamino)xanthen-3-ylidene]-dimethylazanium
• 化学式: C₂₅H₂₂N₃O₃S
• 分子量: 444.534
• InChiKey: IJSMFQNTEUNRPY-UHFFFAOYSA-O

计算性质

• 辛醇/水分配系数(LogP)：
  4.4
• 重原子数：
  32
• 可旋转键数：
  4
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.16
• 拓扑面积：
  97.2
• 氢给体数：
  1
• 氢受体数：
  6

反应信息

• 作为反应物：
  描述：

  9-(2-Carboxy-4-isothiocyanato-phenyl)-3,6-bis-dimethylamino-xanthenylium 、 正丁胺 以 二氯甲烷 为溶剂， 生成 {9-[4-(3-Butyl-thioureido)-2-carboxy-phenyl]-6-dimethylamino-xanthen-3-ylidene}-dimethyl-ammonium
  参考文献：
  名称：

  Molecular Tripods Showing Fluorescence Enhancement upon Binding to Streptavidin

  摘要：

  We introduce a new approach to biotin-streptavidin assays based on a molecular tripod which consists of biotin, a fluorophore, and a quencher. The interaction between streptavidin and molecular tripods perturbs the ground-state quencher-fluorophore dimeric conformation in the absence of streptavidin and diminishes the intrinsic self-quenching of a quencher-fluorophore pair. The emission intensity of the molecular tripods plus streptavidin is 3.5-5.2 times that of molecular tripods in the absence of streptavidin.

  DOI：

  10.1021/ol047801h

文献信息

• Solid-Phase Oligosaccharide Tagging: a Technique for Manipulation of Immobilized Carbohydrates
  申请人：Lohse Anders

  公开号：US20080227092A1

  公开（公告）日：2008-09-18

  The invention relates to methods of manipulating immobilised carbohydrates by derivatisation. Depending on the nature of the derivatisation, the carbohydrate may thereby be more easily detected and/or identified or handled. In particular, the invention relates to methods of preparing a reactive sugar comprising the steps of: i) providing a sample comprising a reducing sugar; ii) providing a solid support covalently attached to a linker comprising a capture group comprising an —NH2 group, wherein said linker optionally is attached to said solid support via a spacer; iii) reacting said reducing sugar with said —NH2 group, thereby obtaining an immobilised sugar; iv) reacting free —NH2 groups with a capping agent, wherein the capping agent comprises a reactive group capable of reacting with an —NH2 group; and v) reducing C═N bonds with a reducing agent, thereby obtaining an reactive sugar of the structure SugarCH n —NH— linked to a solid support via a linker and optionally a spacer, wherein n is 1 or 2.

  本发明涉及通过衍生化来操纵固定化碳水化合物的方法。根据衍生化的性质，碳水化合物可以更容易地被检测和/或识别或处理。特别地，本发明涉及制备反应性糖的方法，包括以下步骤：i）提供包含还原糖的样品；ii）提供共价连接到捕获基团（包括—NH2基团）的连接剂的固体支持，其中所述连接剂可以通过间隔物连接到所述固体支持；iii）将还原糖与所述—NH2基团反应，从而获得固定化糖；iv）用盖帽剂反应自由—NH2基团，所述盖帽剂包括能够与—NH2基团反应的反应性基团；v）用还原剂还原C═N键，从而获得结构为SugarCHn—NH—的反应性糖，其通过连接剂和可选的间隔物与固体支持连接，其中n为1或2。
• US9116128B2
  申请人：——

  公开号：US9116128B2

  公开（公告）日：2015-08-25