(3,4-Diethyl-2-nitrophenoxy)-dihydroxy-sulfanylidene-lambda5-phosphane

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 有机硫代磷酸及其衍生物
• 英文名称: (3,4-Diethyl-2-nitrophenoxy)-dihydroxy-sulfanylidene-lambda5-phosphane
• 英文别名: (3,4-diethyl-2-nitrophenoxy)-dihydroxy-sulfanylidene-λ5-phosphane
• 化学式: C10H14NO5PS
• 分子量: 291.26
• InChiKey: DMNOHCHQCVSKLT-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.4
• 重原子数：
  18
• 可旋转键数：
  4
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.4
• 拓扑面积：
  128
• 氢给体数：
  2
• 氢受体数：
  6

文献信息

• Method for constructing a plasma DNA sequencing library
  申请人：Berry Genomics Co., Ltd.

  公开号：US10227587B2

  公开（公告）日：2019-03-12

  The disclosure relates to a method for constructing a plasma Deoxyribonucleic acid (DNA) sequencing library. The method includes: extracting a plasma DNA; making the plasma DNA ligate to a sequencing linker, and purifying a ligation product; performing Polymerase Chain Reaction (PCR) amplification for the purified ligation product, purifying the PCR amplification product, and obtaining the plasma DNA sequencing library, wherein, the method does not include the step of performing 5′-terminus phosphorylation for the plasma DNA. The kit provided by the disclosure includes: a reagent which ligates a plasma DNA to a sequencing linker, including the sequencing linker, a ligase and a ligation buffer; and reagents and instruments for purifying the ligation product; a reagent which performs PCR amplification for a purified ligation product, and reagents and instruments for purifying the PCR amplification product; wherein, the kit does not include the reagent which performs 5′-terminus phosphorylation for the plasma DNA.

  本公开涉及一种构建血浆脱氧核糖核酸（DNA）测序文库的方法。该方法包括：提取血浆 DNA；使血浆 DNA 与测序连接子连接，纯化连接产物；对纯化的连接产物进行聚合酶链反应（PCR）扩增，纯化 PCR 扩增产物，获得血浆 DNA 测序文库，其中，该方法不包括对血浆 DNA 进行 5′-末端磷酸化的步骤。本公开提供的试剂盒包括：将血浆DNA与测序连接体连接的试剂，包括测序连接体、连接酶和连接缓冲液；以及纯化连接产物的试剂和仪器；对纯化的连接产物进行PCR扩增的试剂，以及纯化PCR扩增产物的试剂和仪器；其中，试剂盒不包括对血浆DNA进行5′-末端磷酸化的试剂。
• METHOD AND KIT FOR CONSTRUCTING PLASMA DNA SEQUENCING LIBRARY
  申请人：Zhang Jianguang

  公开号：US20140228256A1

  公开（公告）日：2014-08-14

  The disclosure relates a method and a kit for constructing a plasma Deoxyribonucleic acid (DNA) sequencing library. The method provided by the disclosure includes: extracting a plasma DNA; making the plasma DNA ligate to a sequencing linker, and purifying a ligation product; performing Polymerase Chain Reaction (PCR) amplification for the purified ligation product, purifying the PCR amplification product, and obtaining the plasma DNA sequencing library, wherein, the method does not include the step of performing 5′-terminus phosphorylation for the plasma DNA. The kit provided by the disclosure includes: a reagent which ligates a plasma DNA to a sequencing linker, including the sequencing linker, a ligase and a ligation buffer; and reagents and instruments for purifying the ligation product; a reagent which performs PCR amplification for a purified ligation product, and reagents and instruments for purifying the PCR amplification product; wherein, the kit does not include the reagent which performs 5′-terminus phosphorylation for the plasma DNA. The disclosure simplifies the construction flow of a plasma DNA sequencing library, simplifies the experimental procedures, and makes the construction of the plasma sample library have lower cost, higher efficiency and faster speed, and is convenient for large-scale application.
• METHOD FOR CONSTRUCTING A PLASMA DNA SEQUENCING LIBRARY
  申请人：Zhang Jianguang

  公开号：US20180142235A1

  公开（公告）日：2018-05-24

  The disclosure relates a method for constructing a plasma Deoxyribonucleic acid (DNA) sequencing library. The method includes: extracting a plasma DNA; making the plasma DNA ligate to a sequencing linker, and purifying a ligation product; performing Polymerase Chain Reaction (PCR) amplification for the purified ligation product, purifying the PCR amplification product, and obtaining the plasma DNA sequencing library, wherein, the method does not include the step of performing 5′-terminus phosphorylation for the plasma DNA. The kit provided by the disclosure includes: a reagent which ligates a plasma DNA to a sequencing linker, including the sequencing linker, a ligase and a ligation buffer; and reagents and instruments for purifying the ligation product; a reagent which performs PCR amplification for a purified ligation product, and reagents and instruments for purifying the PCR amplification product; wherein, the kit does not include the reagent which performs 5′-terminus phosphorylation for the plasma DNA.