[EN] METHOD AND APPARATUS FOR TIME-RESOLVED FLUORESCENCE IMMUNOASSAY TESTING<br/>[FR] PROCÉDÉ ET APPAREIL POUR EFFECTUER UN IMMUNO-ESSAI PAR FLUORESCENCE À RÉSOLUTION TEMPORELLE
申请人:LAND AND LONG INTERNAT TRADING CO LTD
公开号:WO2014146215A1
公开(公告)日:2014-09-25
A method and apparatus for assaying to detect the presence or quantity of an analyte in a test sample, comprising;forming an unbounded aqueous mixture of a first test sample with a defined amount of a nanosphere probe which is conjugate of an analyte capturing member and a long emission fluorescent label, contacting a contacting zone of a test strip with the aqueous mixture, the test area having bound thereat a test binding moiety that for a competition assay binds any sample in competition With the analyte Capturing member; or for a sandwich assay binds any sample analyte non-competitively with the analyte capturing member, the control area having bound a control binding moiety to nanosphere probe, selectively measuring long emission fluorescence at the test and control areas, and for a given test strip, determining a test zone value normalized with the total of test and control area signals.