6'-羧基-2',7'-二氯荧光素 | 144316-86-9

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 中文名称: 6'-羧基-2',7'-二氯荧光素
• 中文别名: 6-羧基-2,7-二氯二氢荧光素
• 英文名称: 6-Carboxy-2',7'-dichlorofluorescein
• 英文别名: 2',7'-dichloro-3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylic acid
• CAS: 144316-86-9
• 化学式: C₂₁H₁₀Cl₂O₇
• 分子量: 445.2
• InChiKey: HLRKQIBTEHWNND-UHFFFAOYSA-N

物化性质

• 沸点：
  742.4±60.0 °C(Predicted)
• 密度：
  1.86±0.1 g/cm3(Predicted)
• 溶解度：
  不溶于水；溶于N,N-二甲基甲酰胺、二甲基亚砜、甲醇
• 最大波长(λmax)：
  495nm (Buffer pH4.0); 504nm (Buffer pH8.0)

计算性质

• 辛醇/水分配系数(LogP)：
  4.2
• 重原子数：
  30
• 可旋转键数：
  1
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.05
• 拓扑面积：
  113
• 氢给体数：
  3
• 氢受体数：
  7

文献信息

• Method for counting living cells of microbes and apparatus therefor
  申请人：MITSUBISHI JUKOGYO KABUSHIKI KAISHA

  公开号：EP0443700A2

  公开（公告）日：1991-08-28

  A method for counting living cells of microbes in a fluid sample continuously while flowing the sample using an apparatus which comprises a system for supplying at a predetermined rate to the flow line of the sample a reagent, such as, a derivative of fluorescein, capable of reacting with one or more substances intrinsic of the living cell, such as enzymes, to form an accumulative fluorescent product within the living cells; a reactor inserted in said flow line of the sample and being provided for said reaction of the reagent with the cell-intrinsic substance in the living cells; a photometric detection system arranged subsequent to said reactor for detecting fluorescence emitted as individual luminous point from the fluorescent product in each of the living cells floating in the flowing sample upon irradiation of the fluorescent product by an exciting ray; and an electronic unit including a pulse counter for counting electric pulses produced from each fluorescence from the luminous point.

  一种在流体样品流动过程中连续计数流体样品中微生物活细胞的方法，该方法使用一种设备，该设备包括一个系统，该系统以预定速率向样品的流线提供一种试剂，如荧光素的衍生物，该试剂能够与活细胞中的一种或多种固有物质（如酶）发生反应，在活细胞内形成累积荧光产物；一个反应器，插入所述样品流管中，用于所述试剂与活细胞中的细胞固有物质发生反应；一个光度检测系统，布置在所述反应器之后，用于检测浮在流动样品中的每个活细胞中的荧光产物在受激光线照射后作为单个发光点发出的荧光；以及一个电子装置，包括一个脉冲计数器，用于计数每个发光点发出的荧光产生的电脉冲。
• METHOD OF DNA SEQUENCING
  申请人：THE INSTITUTE OF PHYSICAL & CHEMICAL RESEARCH

  公开号：EP0978569A1

  公开（公告）日：2000-02-09

  A method of DNA sequencing comprising reacting a ribonucleoside 5'-triphosphate with a 3'dNTP derivative in the presence of a mutated RNA polymerase modified so as to enhance the ability to take up the 3'dNTP derivative and a DNA fragment containing a promoter sequence for the RNA polymerase, separating the nucleic acid transcription product thus obtained, and reading the sequence of the nucleic acid from the fraction thus separated. By using this method, a long-chain transcription product can be formed and more accurate sequence data with little change in the signals from labeled deoxyribonucleotides can be obtained.

  一种 DNA 测序方法，包括在经过修饰以提高吸收 3'dNTP 衍生物能力的突变 RNA 聚合酶和含有 RNA 聚合酶启动子序列的 DNA 片段存在的情况下，使核糖核苷 5'- 三磷酸酯与 3'dNTP 衍生物反应，分离由此获得的核酸转录产物，并从由此分离的部分读取核酸序列。使用这种方法可以形成长链转录产物，获得更准确的序列数据，而标记脱氧核苷酸的信号变化很小。
• 3'-DEOXYRIBONUCLEOTIDE DERIVATIVES
  申请人：THE INSTITUTE OF PHYSICAL & CHEMICAL RESEARCH

  公开号：EP1046648A1

  公开（公告）日：2000-10-25

  3'-Deoxyribonucleotide derivatives represented by the following general formula [I]: Q-V-(CH2)n-NR-R wherein Q represents a 3'-deoxyribonucleotide residue; n is an integer of 4 or more; V represents -C≡C- or -CH=CH-; and R represents a fluorescent group. Because of having been improved in the uptake efficiency by RNA polymerases, these derivatives are useful as terminators in DNA base sequencing methods with the use of RNA polymerases.

  由以下通式[I]代表的 3'-脱氧核苷酸衍生物：Q-V-(CH2)n-NR-R 其中 Q 代表 3'-脱氧核苷酸残基；n 是 4 或更多的整数；V 代表 -C≡C- 或 -CH=CH-；R 代表荧光基团。由于提高了 RNA 聚合酶的吸收效率，这些衍生物在使用 RNA 聚合酶的 DNA 碱基测序方法中可用作终止子。
• Use of fluorescent reagents in identification of cancerous cells and activated lymphocytes
  申请人：——

  公开号：US20010049091A1

  公开（公告）日：2001-12-06

  The present invention provides methods of determining whether test cells in a sample are cancerous or not and determining if lymphocytes are activated or not. The method measures the test cell's DNA ploidy and the cellular activity of an enzyme such as an esterase, which has altered expression in cancer cells. Esterase activity can be measured using fluorescent compounds such as fluorescein diacetate.

  本发明提供了确定样本中的测试细胞是否癌变以及确定淋巴细胞是否活化的方法。该方法可测量检测细胞的 DNA 倍性和一种酶（如酯酶）的细胞活性，酯酶在癌细胞中的表达会发生改变。酯酶活性可通过荧光化合物（如二乙酸荧光素）来测量。
• COLOR-CHANGING CLEANSING COMPOSITIONS AND METHODS
  申请人：Hanes Robert Eugene

  公开号：US20090176673A1

  公开（公告）日：2009-07-09

  The present invention provides methods, kits and compositions that are nontoxic color changing cleansers The cleansers include a mixture having a first component mixture and a second component mixture, wherein the first component mixture comprises a nontoxic color changing dye and the first component mixture in combination with the second component mixture such that the color of the nontoxic color changing dye is of a different color than the nontoxic color changing dye in the first component mixture.