6-(4,6-二氯三嗪基)氨基荧光素 | 82169-58-2

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 中文名称: 6-(4,6-二氯三嗪基)氨基荧光素
• 中文别名: 6-[(4,6-二氯-1,3,5-三嗪-2-基)氨基]-3',6'-二羟基-3H-螺[2-苯并呋喃-1,9'-占吨]-3-酮
• 英文名称: 6-(4,6-Dichlorotriazinyl)aminofluorescein
• 英文别名: 5-[(4,6-dichloro-1,3,5-triazin-2-yl)amino]-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
• CAS: 82169-58-2
• 化学式: C₂₃H₁₂Cl₂N₄O₅
• 分子量: 495.3
• InChiKey: MDGNQWAJTIGWLE-UHFFFAOYSA-N

物化性质

• 熔点：
  >350 °C
• 沸点：
  844.3±75.0 °C(Predicted)
• 密度：
  1.81±0.1 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  5.3
• 重原子数：
  34
• 可旋转键数：
  2
• 环数：
  6.0
• sp3杂化的碳原子比例：
  0.04
• 拓扑面积：
  127
• 氢给体数：
  3
• 氢受体数：
  9

文献信息

• Site-specific aminoglycoside derivatives and their use in immunodiagnostic assays
  申请人：——

  公开号：US20030060430A1

  公开（公告）日：2003-03-27

  A method of making a derivatized aminoglycoside includes reacting an aminoglycoside with at least 2 equivalents of a divalent metal ion in an aprotic solvent to complex two neighboring amino group and hydroxyl group pairs; reacting the non-complexed amino groups with a protecting reagent to provide protecting groups; removing the divalent metal ion to provide two unprotected amino groups; reacting one of the unprotected amino groups with a reactive substance containing an linker, a carrier, or a label; and removing the protecting groups. This method can be used to produce novel compounds and reagents.

  制备衍生氨基糖苷的方法包括在无极性溶剂中将氨基糖苷与至少2当量的二价金属离子反应，以形成两个相邻氨基和羟基对的络合物；将未形成络合物的氨基基团与保护试剂反应以提供保护基团；去除二价金属离子以提供两个未受保护的氨基基团；将其中一个未受保护的氨基基团与含有连接剂、载体或标记的反应物质反应；最后去除保护基团。这种方法可以用来生产新颖的化合物和试剂。
• Procainamide assay, tracers, immunogens and antibodies
  申请人：ABBOTT LABORATORIES

  公开号：EP0199042A1

  公开（公告）日：1986-10-29

  The present invention is directed to a fluorescence polarization immunoassay for procainamide, to the various components needed for preparing and carrying out such an assay, and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for making them. The tracers and the immunogens are made from analogs of procainamide. A fluorescein moiety is included in the tracer while a poly(amino acid) forms a part of the immunogen. The assay is conducted by measuring the degree of ' polarization retention of plane polarized light that has been passed through a sample containing antiserum and tracer.

  该发明涉及一种用于普鲁卡因胺的荧光偏振免疫分析，以及制备和执行此类分析所需的各种组分，以及制造这些组分的方法。具体地，揭示了示踪剂、免疫原和抗体，以及制造它们的方法。示踪剂和免疫原是由普鲁卡因胺的类似物制成的。示踪剂中包含荧光基团，而多肽（氨基酸）是免疫原的一部分。该分析通过测量通过含有抗血清和示踪剂的样品的偏振保留度来进行，该样品已经通过平面偏振光。
• Site-specific aminoglycoside derivatives for use in immunodiagnostic assays
  申请人：——

  公开号：US20040138425A1

  公开（公告）日：2004-07-15

  A method of making a derivatized aminoglycoside includes reacting an aminoglycoside with at least 2 equivalents of a divalent metal ion in an aprotic solvent to complex two neighboring amino group and hydroxyl group pairs; reacting the non-complexed amino groups with a protecting reagent to provide protecting groups; removing the divalent metal ion to provide two unprotected amino groups; reacting one of the unprotected amino groups with a reactive substance containing an linker, a carrier, or a label; and removing the protecting groups. This method can be used to produce novel compounds and reagents.

  一种制备衍生氨基糖苷的方法，包括在无质子溶剂中将氨基糖苷与至少2当量的二价金属离子反应，形成两个相邻氨基和羟基对的络合物；将未络合的氨基基团与保护试剂反应，提供保护基；去除二价金属离子，得到两个未保护的氨基基团；将其中一个未保护的氨基基团与含有连接剂、载体或标记的反应性物质反应；去除保护基。该方法可用于生产新的化合物和试剂。
• SITE-SPECIFIC AMINOGLYCOSIDE DERIVATIVES FOR USE IN IMMUNODIAGNOSTIC ASSAYS
  申请人：Ghoshal Mitali

  公开号：US20070148700A1

  公开（公告）日：2007-06-28

  A method of making a derivatized aminoglycoside includes reacting an aminoglycoside with at least 2 equivalents of a divalent metal ion in an aprotic solvent to complex two neighboring amino group and hydroxyl group pairs; reacting the non-complexed amino groups with a protecting reagent to provide protecting groups; removing the divalent metal ion to provide two unprotected amino groups; reacting one of the unprotected amino groups with a reactive substance containing an linker, a carrier, or a label; and removing the protecting groups. This method can be used to produce novel compounds and regents.

  制备衍生化氨基糖苷的方法包括在无质子溶剂中将氨基糖苷与至少2当量的二价金属离子反应，形成两个相邻氨基和羟基对的络合物；将未络合的氨基与保护试剂反应以提供保护基团；去除二价金属离子以提供两个未保护的氨基；将其中一个未保护的氨基与含有连接剂、载体或标记的反应物质反应；最后去除保护基团。该方法可用于生产新型化合物和试剂。
• Tracers for disopyramide assay and immunogens to raise antibody
  申请人：ABBOTT LABORATORIES

  公开号：EP0184120A2

  公开（公告）日：1986-06-11

  This invention relates to an improved fluorescent polarization assay for disopyramide, and to improved reagents (tracer compounds and immunogen) for use in the assay. The 10 tracer compounds are the reaction product of p- or m-aminodisopyramide with 10 fluorescein compounds. The tracers form complexes with antiserum specific to disopyramide, in proportion to the relative concentration of the tracer with respect to the disopyramide present in the sample. Excellent results are obtained when such a tracer compound is used in fluorescent polarization assay for disopyramide, with greater speed and less cross-reactivity than prior art methods of immunoassay.

  本发明涉及一种改进的荧光偏振测定法，用于测定地索比酰胺和改进的试剂（示踪化合物和免疫原）。10 种示踪化合物是对氨基或间氨基异吡酰胺与 10 种荧光素化合物的反应产物。示踪剂与抗血清特异性地形成复合物，复合物与示踪剂相对于样品中二吡酰胺的相对浓度成比例。与现有技术的免疫测定方法相比，这种示踪化合物在荧光偏振法测定地辛酰胺时，速度更快，交叉反应更少，可获得极佳的结果。