[6-(Diethylamino)-9-[2-[[4-(1,10-phenanthrolin-5-ylcarbamoyl)phenyl]methoxycarbonyl]phenyl]xanthen-3-ylidene]-diethylazanium

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 菲咯啉
• 英文名称: [6-(Diethylamino)-9-[2-[[4-(1,10-phenanthrolin-5-ylcarbamoyl)phenyl]methoxycarbonyl]phenyl]xanthen-3-ylidene]-diethylazanium
• 英文别名: [6-(diethylamino)-9-[2-[[4-(1,10-phenanthrolin-5-ylcarbamoyl)phenyl]methoxycarbonyl]phenyl]xanthen-3-ylidene]-diethylazanium
• 化学式: C48H44N5O4+
• 分子量: 754.9
• InChiKey: CEQFQARZEZRTQS-UHFFFAOYSA-O

计算性质

• 辛醇/水分配系数(LogP)：
  7.5
• 重原子数：
  57
• 可旋转键数：
  12
• 环数：
  8.0
• sp3杂化的碳原子比例：
  0.19
• 拓扑面积：
  96.7
• 氢给体数：
  1
• 氢受体数：
  7

文献信息

• NOVEL TEMPERATURE-SENSITIVE FLUORESCENT COMPOUND AND APPLICATION THEREOF
  申请人：SHANGHAI INSTITUTES FOR BIOLOGICAL SCIENCES, CHINESE ACADEMY OF SCIENCES

  公开号：US20160376247A1

  公开（公告）日：2016-12-29

  Provided is a compound represented by formula I, wherein R 9 is a C1-C22 hydrocarbyl group, or a C2-C3 ester-substituted C1-C3 alkyl group, R 5 , R 6 , R 7 , and R 8 are alkyl groups or H, and R 1 , R 2 , R 3 , and R 4 are H or a lower hydrocarbyl group. Or, R 9 is a C2-C22 hydrocarbyl group, or a C2-C3 ester-substituted C1-C3 alkyl group, and R 5 is connected to R 1 , R 6 is connected to R 2 , R 7 is connected to R 3 , R 8 is connected to R 4 to form six-membered rings. The present compound is temperature-sensitive and can enter into cells. An intracellular temperature distribution image having high spatial and temporal resolution can thus be obtained. The present compound can also perform distribution calibration on a temperature-sensitive fluorescent compound. Also provided is a method for measuring temperature distribution within a living cell, and a corresponding detection kit. The method satisfies the requirements of small size measurement and rapid measurement, thereby achieving high resolution in terms of space and time.

  提供的化合物由式I表示，其中R9是一个C1-C22烃基团，或者是一个C2-C3酯基取代的C1-C3烷基基团，R5、R6、R7和R8是烷基基团或氢，而R1、R2、R3和R4是氢或较低烃基基团。或者，R9是一个C2-C22烃基团，或者是一个C2-C3酯基取代的C1-C3烷基基团，而R5连接到R1，R6连接到R2，R7连接到R3，R8连接到R4形成六元环。这种化合物对温度敏感，可以进入细胞。因此，可以获得具有高空间和时间分辨率的细胞内温度分布图像。这种化合物还可以对温度敏感的荧光化合物进行分布校准。还提供了一种测量活细胞内温度分布的方法和相应的检测工具包。该方法满足了小尺寸测量和快速测量的要求，从而在空间和时间上实现高分辨率。