4,7,2',4',5',7'-hexachloro-5-carboxy-fluorescein | 155911-15-2

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 英文名称: 4,7,2',4',5',7'-hexachloro-5-carboxy-fluorescein
• 英文别名: 2',4,4',5',7,7'-Hexachloro-3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid；2',4,4',5',7,7'-hexachloro-3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
• CAS: 155911-15-2
• 化学式: C₂₁H₆Cl₆O₇
• 分子量: 582.992
• InChiKey: KZCRVHZQAGAFID-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  6.7
• 重原子数：
  34
• 可旋转键数：
  1
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.05
• 拓扑面积：
  113
• 氢给体数：
  3
• 氢受体数：
  7

反应信息

• 作为产物：
  描述：

  2',4',5',7'-tetrachloro-5-carboxy-4,7-dichlorofluorescein diisopropylamine salt 以 水 、 乙腈 为溶剂， 以91 %的产率得到4,7,2',4',5',7'-hexachloro-5-carboxy-fluorescein
  参考文献：
  名称：

  CN116589475

  摘要：

  公开号：

文献信息

• Synthesis and biological applications of two novel fluorescent proteins-labeling probes
  作者：Xiang-long Wu、Min Tian、Huai-zhen He、Wei Sun、Jian-li Li、Zhen Shi

  DOI：10.1016/j.bmcl.2009.04.049

  日期：2009.6

  Two novel chlorinated fluoresceins 2′,4′,5′,7′-tetrachloro-6-(5-carboxypentyl)-4,7-dichloro fluorescein succinimidyl ester (1G) and 2′,4′,5′,7′-tetrachloro-6-(3-carboxypropyl)-4,7-dichlorofluorescein succinimidyl ester (2G) were synthesized as fluorescent probes for labeling proteins. Structures of target compounds and intermediates were determined via IR, MS, 1H NMR and element analysis. The investigation

  两种新型氯化荧光素2'，4'，5'，7'-四氯-6-（5-羧基戊基）-4,7-二氯荧光素琥珀酰亚胺酯（1G）和2'，4'，5'，7'-合成了四氯-6-（3-羧丙基）-4,7-二氯荧光素琥珀酰亚胺酯（2G）作为标记蛋白质的荧光探针。通过IR，MS，1 H NMR和元素分析确定目标化合物和中间体的结构。免疫荧光组织化学研究表明，它们具有较强的荧光性，较高的光稳定性和良好的生物相容性。
• Propargyl substituted nucleoside compounds and methods
  申请人：Rosenblum B. Barnett

  公开号：US20050170388A1

  公开（公告）日：2005-08-04

  The present teachings relate to nucleobase, nucleoside and nucleotide compounds, methods of synthesis, and uses thereof. The present teachings provide compounds, such as nucleobase, nucleoside and/or nucleotide compounds including a propargyl linker, and methods for making or using such compounds.

  本文涉及核碱基、核苷和核苷酸化合物、合成方法和其用途。本文提供了化合物，例如核碱基、核苷和/或核苷酸化合物，包括丙炔基连接物，并提供制备或使用这些化合物的方法。
• Mobility-modified nucleobase polymers and methods of using same
  申请人：Woo L. Sam

  公开号：US20050042644A1

  公开（公告）日：2005-02-24

  The present invention relates generally to nucleobase polymer functionalizing reagents, to mobility-modified sequence-specific nucleobase polymers, to compositions comprising a plurality of mobility-modified sequence-specific nucleobase polymers, and to the use of such polymers and compositions in a variety of assays, such as, for example, for the detection of a plurality of selected nucleotide sequences within one or more target nucleic acids. The mobility-modifying polymers of the present invention include phosphoramidite reagents which can be joined to other mobility-modifying monomers and to sequence-specific oligonucleobase polymers via uncharged phosphate triester linkages. Addition of the mobility-modifying phosphoramidite reagents of the present invention to oligonucleobase polymers results in unexpectedly large effects the mobility of those modified oligonucleobase polymers, especially upon capillary electrophoresis in non-sieving media.

  本发明涉及核碱基聚合物功能化试剂，改变运动性的序列特异性核碱基聚合物，包含多种改变运动性的序列特异性核碱基聚合物的组合物，以及在多种检测中使用这些聚合物和组合物，例如，在一个或多个目标核酸中检测多个选定的核苷酸序列。本发明的改变运动性的聚合物包括磷酰胺试剂，可以通过不带电的磷酸三酯键连接到其他改变运动性的单体和序列特异性寡核苷酸聚合物。将本发明的改变运动性的磷酰胺试剂添加到寡核苷酸聚合物中，会出现意外的大效应，特别是在非筛选介质中进行毛细管电泳。
• METHOD FOR REPLICATING NUCLEIC ACIDS AND NOVEL UNNATURAL BASE PAIRS
  申请人：Hirao Ichiro

  公开号：US20100036111A1

  公开（公告）日：2010-02-11

  The present invention relates to a method for nucleic acid replication and novel artificial base pairs. The method of the present invention for nucleic acid replication is characterized in that a deoxyribonucleoside 5′-triphosphate, in which the hydroxyl group of phosphoric acid at the γ-position is substituted with a group selected from the group consisting of an amino group, a methylamino group, a dimethylamino group, a mercapto group and a fluoro group, is used as a substrate during replication reaction. The novel artificial base pairs of the present invention are characterized in that 7-(2-thienyl)-imidazo[4,5-b]pyridine (Ds) or an analog thereof forms a base pair with pyrrole-2-carbaldehyde (Pa) or an analog thereof.

  本发明涉及一种核酸复制方法和新型人工碱基对。本发明的核酸复制方法的特征在于，在复制反应过程中使用一种脱氧核苷酸5'-三磷酸作为底物，其中磷酸γ-位置的羟基被从以下群组中选择的一种群组所取代，所述群组包括氨基、甲基氨基、二甲基氨基、巯基和氟基。本发明的新型人工碱基对的特征在于，7-(2-噻吩基)-咪唑并[4,5-b]吡啶（Ds）或其类似物与吡咯-2-甲醛（Pa）或其类似物形成一对碱基。
• NOVEL DNA CAPABLE OF BEING AMPLIFIED BY PCR WITH HIGH SELECTIVITY AND HIGH EFFICIENCY
  申请人：Hirao Ichiro

  公开号：US20110053782A1

  公开（公告）日：2011-03-03

  The present invention relates to unnatural base pairs of Ds (a 5-amino-7-(2-thienyl)-3H-imidazo[4,5-b]pyridine-3-yl group) and a Pa derivative (a 2-nitro-1H-pyrrole-1-yl group bearing a substituent having a π-electron system attached at position 4) that can be replicated with high selectivity/high efficiency, and methods for replicating nucleic acids containing the unnatural base pairs. The present invention also relates to methods for incorporating an unnatural base bearing a functional substituent attached thereto into DNA by a nucleic acid replication reaction. The present invention also relates to methods for replicating and selectively collecting a nucleic acid containing an unnatural base pair from a nucleic acid pool. The present invention also relates to methods for determining a sequence of natural bases in the proximity of an unnatural base in DNA for achieving highly efficient and highly selective replication of a nucleic acid containing the unnatural base.

  本发明涉及Ds不自然碱基对（一种含有5-氨基-7-（2-噻吩基）-3H-咪唑[4,5-b]吡啶-3-基团的化合物）和Pa衍生物（一种2-硝基-1H-吡咯烷-1-基团，其在4位带有π电子体系的取代基）的高选择性/高效率复制，以及包含这些不自然碱基对的核酸的复制方法。本发明还涉及通过核酸复制反应将带有功能性取代基的不自然碱基引入DNA的方法。本发明还涉及从核酸池中复制和选择性收集含有不自然碱基对的核酸的方法。本发明还涉及确定DNA中不自然碱基附近自然碱基的序列以实现高效和高选择性复制含有不自然碱基的核酸的方法。