7-[(4,6-Dichloro-1,3,5-triazin-2-yl)amino]-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 英文名称: 7-[(4,6-Dichloro-1,3,5-triazin-2-yl)amino]-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
• 化学式: C₂₃H₁₂Cl₂N₄O₅
• 分子量: 495.3
• InChiKey: QSWHRQGKGDZSPH-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  5.8
• 重原子数：
  34
• 可旋转键数：
  2
• 环数：
  6.0
• sp3杂化的碳原子比例：
  0.04
• 拓扑面积：
  127
• 氢给体数：
  3
• 氢受体数：
  9

文献信息

• METHOD FOR DETERMINATION OF ENZYMATIC ACTIVITY
  申请人：Yamamoto Koji

  公开号：US20090104627A1

  公开（公告）日：2009-04-23

  A novel modified polysaccharide, a solid phase to which the polysaccharide is adhered, methods for detecting N-deacetylase activity, N-sulfotransferase activity and N-deacetylase/N-sulfotransferase activity in a sample which utilizes said solid phase, and detection kits thereof.

  一种新型改性多糖，其中多糖附着在固相上，用于检测样品中N-脱乙酰酰胺酶活性、N-硫酰转移酶活性和N-脱乙酰酰胺酶/N-硫酰转移酶活性的方法，以及使用该固相的检测工具箱。
• Chromogenic tracers for use in an assay
  申请人：Becton, Dickinson and Company

  公开号：EP0115332A2

  公开（公告）日：1984-08-08

  A chromogen, in particular, a fluorescent compound, is conjugated to a ligand through an amino acid spacer radial, which includes a free carboxyl group. The tracers are particularly suitable for use in a flow through assay for low molecular weight analytes. The tracers are rapidly bound, of good sensitivity, and low non-specific binding.

  显色剂，特别是荧光化合物，通过包括游离羧基的氨基酸间隔径向与配体共轭。 这种示踪剂特别适用于低分子量分析物的流动检测。 这种示踪剂结合迅速，灵敏度高，非特异性结合率低。
• Fluorescence polarization assay for macromolecular hydrolases.
  申请人：ABBOTT LABORATORIES

  公开号：EP0194472A2

  公开（公告）日：1986-09-17

  A fluorescence polarization assay for determining the presence and/or amount of macromolecular hydrolases such as polysaccharide hydrolases, amylase, trypsin, proteases and lipases is disclosed. The disclosure provides reagents, processes for making the reagents useful in the assay and processes for conducting the assay. The assay involves the use of a substrate capable of being cleaved by the macromolecular hydrolase being determined, wherein the substrate is coupled to a fluorophore.

  本发明公开了一种荧光偏振测定法，用于确定多糖水解酶、淀粉酶、胰蛋白酶、蛋白酶和脂肪酶等大分子水解酶的存在和/或数量。本发明提供了试剂、试剂的制备方法和试剂的检测方法。该检测涉及使用能够被正在测定的大分子水解酶裂解的底物，其中底物与荧光团耦合。
• Tracers for use in flecainide fluorescence polarization immunoassay
  申请人：ABBOTT LABORATORIES

  公开号：EP0242847A2

  公开（公告）日：1987-10-28

  The present invention is directed to a fluorescence polarization assay for determining the amount of flecainide in fluids, especially biological fluids such as serum, plasma or urine. The present invention also relates to novel tracer compounds useful in fluorescence polarization assay techniques.

  本发明涉及一种荧光偏振测定法，用于确定体液，特别是血清、血浆或尿液等生物体液中的非加尼含量。 本发明还涉及可用于荧光偏振测定技术的新型示踪化合物。
• Immunoassay for opiate alkaloids and their metabolites; tracers, immunogens and antibodies
  申请人：ABBOTT LABORATORIES

  公开号：EP0254120A2

  公开（公告）日：1988-01-27

  The present invention is directed to a fluorescence polarization assay for opiate alkaloids and their metabolites, to the various components needed for preparing and carrying out such an assay and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for making them. The tracers and the immunogens are made from substituted opiate alkaloids. A fluorescein moiety is included in the tracers, while a poly(amino acid) forms a part of the immunogens. The assay is conducted by measuring the degree of polarization retention of the fluorescence resulting when a sample mixed with antiserum and tracer is irradiated with plane-polarized light.

  本发明涉及鸦片生物碱及其代谢物的荧光偏振测定法、制备和进行这种测定法所需的各种成分以及制造这些成分的方法。 具体地说，本发明公开了示踪剂、免疫原和抗体以及制造它们的方法。 示踪剂和免疫原是由取代的鸦片生物碱制成的。 示踪剂中含有荧光素分子，而免疫原中含有聚氨基酸。 该测定法是通过测量当用平面偏振光照射与抗血清和示踪剂混合的样品时所产生的荧光的偏振保留程度来进行的。