申请人:HSC Research Development Corporation
公开号:US04772563A1
公开(公告)日:1988-09-20
1,10-phenanthroline-2,9-dicarboxylic acid and novel derivatives thereof such as 4,7-diphenyl-1,10-phenanthroline-2,9-dicarboxylic acid and 4,5,9,14-tetraaza-(1,2,3,4)-dibenzanthracene-3,5-dicarboxylic acid can be coupled to proteins through coupling groups to form conjugates which form higly fluorescent chelates in the presence of lanthanide salts. Derivatives of the above acids reactive toward proteins can be readily manufactured by relatively simple synthetic routes, and are useful in fluorescent immunoassay.
1,10-菲咯啉-2,9-二羧酸及其新的衍生物,例如4,7-二苯基-1,10-菲咯啉-2,9-二羧酸和4,5,9,14-四氮杂-(1,2,3,4)-二苯并蒽-3,5-二羧酸,可以通过偶联基与蛋白质结合形成共轭物,当与镧系盐共存时形成高度荧光螯合物。上述酸的蛋白质反应性衍生物可以通过相对简单的合成路线轻松制备,并且在荧光免疫测定中非常有用。