β-D-5'-O-tert-butyldimethylsilyl-N⁴,3'-O-bis(4-methoxytrityl)-2'-deoxycytidine | 250581-87-4

• 分子结构分类: 有机化合物 - 苯类化合物 - 三苯基化合物
• 英文名称: β-D-5'-O-tert-butyldimethylsilyl-N⁴,3'-O-bis(4-methoxytrityl)-2'-deoxycytidine
• 英文别名: 1-[(2R,4S,5R)-5-[[tert-butyl(dimethyl)silyl]oxymethyl]-4-[(4-methoxyphenyl)-diphenylmethoxy]oxolan-2-yl]-4-[[(4-methoxyphenyl)-diphenylmethyl]amino]pyrimidin-2-one
• CAS: 250581-87-4
• 化学式: C₅₅H₅₉N₃O₆Si
• 分子量: 886.175
• InChiKey: OYDICUFILFWTSH-GUPVCERZSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  11.35
• 重原子数：
  65
• 可旋转键数：
  17
• 环数：
  8.0
• sp3杂化的碳原子比例：
  0.27
• 拓扑面积：
  90.8
• 氢给体数：
  1
• 氢受体数：
  6

反应信息

• 作为反应物：
  描述：

  β-D-5'-O-tert-butyldimethylsilyl-N⁴,3'-O-bis(4-methoxytrityl)-2'-deoxycytidine 在 四丁基氟化铵 作用下， 以 四氢呋喃 为溶剂， 反应 2.0h， 以88%的产率得到β-D-N⁴,3'-O-bis(4-methoxytrityl)-2'-deoxycytidine
  参考文献：
  名称：

  Study of human deoxycytidine kinase binding properties using intrinsic fluorescence or new fluorescent ligands

  摘要：

  A series of D- and L-enantiomers of cytidine or adenosine analogues and fluorescent N-methylanthraniloyl (MeNHBz) cytidine derivatives regiospecifically synthesized from cytidine or deoxycytidine were used to quantify the enantioselectivity of human deoxycytidine kinase (dCK) and to characterize its binding states. Both D- and L-enantiomers of these compounds induced significant bimodal quenchings of the intrinsic fluorescence of the enzyme. The ratios of dissociation constants Kd(D)/Kd(L) for the high affinity binding of non fluorescent cytidine derivatives were remarkably similar. beta-D- and beta-L-ATP gave monophasic titration curves and the enzyme displayed a preference for the natural enantiomer. This demonstrates the lack of enantioselectivity of dCK in the substrate binding steps of its mechanism. The results of other fluorescence experiments with MeNHBz-cytidine derivatives were consistent with an enzyme mechanism in which nucleotide binding precedes nucleoside binding. (C) Elsevier, Paris.

  DOI：

  10.1016/s0223-5234(99)80092-0
• 作为产物：
  描述：

  4-甲氧基三苯基氯甲烷 、 5'-O-(tert-butyldimethylsilyl)-2'-deoxycytidine 以61%的产率得到β-D-5'-O-tert-butyldimethylsilyl-N⁴,3'-O-bis(4-methoxytrityl)-2'-deoxycytidine
  参考文献：
  名称：

  Study of human deoxycytidine kinase binding properties using intrinsic fluorescence or new fluorescent ligands

  摘要：

  A series of D- and L-enantiomers of cytidine or adenosine analogues and fluorescent N-methylanthraniloyl (MeNHBz) cytidine derivatives regiospecifically synthesized from cytidine or deoxycytidine were used to quantify the enantioselectivity of human deoxycytidine kinase (dCK) and to characterize its binding states. Both D- and L-enantiomers of these compounds induced significant bimodal quenchings of the intrinsic fluorescence of the enzyme. The ratios of dissociation constants Kd(D)/Kd(L) for the high affinity binding of non fluorescent cytidine derivatives were remarkably similar. beta-D- and beta-L-ATP gave monophasic titration curves and the enzyme displayed a preference for the natural enantiomer. This demonstrates the lack of enantioselectivity of dCK in the substrate binding steps of its mechanism. The results of other fluorescence experiments with MeNHBz-cytidine derivatives were consistent with an enzyme mechanism in which nucleotide binding precedes nucleoside binding. (C) Elsevier, Paris.

  DOI：

  10.1016/s0223-5234(99)80092-0

文献信息

• Study of human deoxycytidine kinase binding properties using intrinsic fluorescence or new fluorescent ligands
  作者：Manijeh Shafiee、Gilles Gosselin、Jean-Louis Imbach、Gilles Divita、Staffan Eriksson、Georges Maury

  DOI：10.1016/s0223-5234(99)80092-0

  日期：1999.5

  A series of D- and L-enantiomers of cytidine or adenosine analogues and fluorescent N-methylanthraniloyl (MeNHBz) cytidine derivatives regiospecifically synthesized from cytidine or deoxycytidine were used to quantify the enantioselectivity of human deoxycytidine kinase (dCK) and to characterize its binding states. Both D- and L-enantiomers of these compounds induced significant bimodal quenchings of the intrinsic fluorescence of the enzyme. The ratios of dissociation constants Kd(D)/Kd(L) for the high affinity binding of non fluorescent cytidine derivatives were remarkably similar. beta-D- and beta-L-ATP gave monophasic titration curves and the enzyme displayed a preference for the natural enantiomer. This demonstrates the lack of enantioselectivity of dCK in the substrate binding steps of its mechanism. The results of other fluorescence experiments with MeNHBz-cytidine derivatives were consistent with an enzyme mechanism in which nucleotide binding precedes nucleoside binding. (C) Elsevier, Paris.