2-[[[4-(苯基偶氮)苯基]甲基]硫代]乙醇 | 84712-96-9

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 偶氮苯
• 中文名称: 2-[[[4-(苯基偶氮)苯基]甲基]硫代]乙醇
• 英文名称: 2-[[[4-(Phenylazo)phenyl]methyl]thio]ethanol
• 英文别名: 2-[(4-phenyldiazenylphenyl)methylsulfanyl]ethanol
• CAS: 84712-96-9
• 化学式: C₁₅H₁₆N₂OS
• 分子量: 272.371
• InChiKey: AZPMLJUDKMGFPX-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.5
• 重原子数：
  19
• 可旋转键数：
  6
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.2
• 拓扑面积：
  70.2
• 氢给体数：
  1
• 氢受体数：
  4

反应信息

• 作为反应物：
  描述：

  2-[[[4-(苯基偶氮)苯基]甲基]硫代]乙醇 在 4-二甲氨基吡啶 、 三乙胺 、 N,N'-二环己基碳二亚胺 、 三氟乙酸 作用下， 以 二氯甲烷 、 N,N-二甲基甲酰胺 为溶剂， 反应 6.41h， 生成 2-[(4-phenyldiazenylphenyl)methylsulfanyl]ethyl 6-[[1-methyl-4-[(2-methylpropan-2-yl)oxycarbonylamino]pyrrole-2-carbonyl]amino]hexanoate
  参考文献：
  名称：

  Sinyakov; Ryabinin; Seregin, Russian Journal of Bioorganic Chemistry, 1997, vol. 23, # 7, p. 502 - 510

  摘要：

  DOI：

文献信息

• Covalently linked oligonucleotide minor grove binder conjugates
  申请人：Microprobe

  公开号：US20020052482A1

  公开（公告）日：2002-05-02

  Minor groove binding molecules are covalently bound to oligonucleotides which in their base sequence are complementary to a target sequence of single stranded or double stranded DNA, RNA or hybrids thereof. The covalently bound oligonucleotide minor groove binder conjugates strogly bind to the target sequence of the complementary strand.

  小沟结合分子与寡核苷酸共价结合，其碱基序列与单链或双链DNA、RNA或其杂交体的目标序列互补。共价结合的寡核苷酸小沟结合物与互补链的目标序列强烈结合。
• Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
  申请人：Hedgpeth Joel

  公开号：US20050064463A1

  公开（公告）日：2005-03-24

  Conjugates between a minor groove binding molecule, such as the trimer of 1,2-dihydro-(3H)-pyrrolo[3,2-e]indole-7-carboxylate (CDPI 3 ), and an oligonucleotide form unusually stable hybrids with complementary target sequences, in which the tethered CDPI 3 group resides in the minor groove of the duplex. These conjugates can be used as probes and primers. Due to their unusually high binding affinity, conjugates as short as 8-mers can be used as amplification primers with high specificity and efficiency. MGB conjugation also increases the discriminatory power of short oligonucleotides, providing enhanced detection of nucleotide sequence mismatches by short oligonucleotides. The MGB-conjugated probes and primers described herein facilitate various analytic and diagnostic procedures, such as amplification reactions, PCR, detection of single-nucleotide polymorphisms, gene hunting, differential display, fluorescence energy transfer, hydrolyzable probe assays and others; by allowing the use of shorter oligonucleotides, which have higher specificity and better discriminatory power.

  一种小沟结合分子（例如1,2-二氢-（3H）-吡咯并[3,2-e]吲哚-7-羧酸三聚体（CDPI3））与寡核苷酸之间的共轭体形成具有不寻常稳定性的杂交体，其中锚定的CDPI3基团驻留在双链的小沟中。这些共轭体可用作探针和引物。由于它们的结合亲和力异常高，因此即使是8-mer长度的共轭体也可以用作具有高特异性和高效率的扩增引物。MGB结合还增加了短寡核苷酸的鉴别能力，通过短寡核苷酸提供了更好的核苷酸序列不匹配检测。在此描述的MGB结合的探针和引物可以促进各种分析和诊断程序，例如扩增反应，PCR，单核苷酸多态性检测，基因猎取，差异显示，荧光能量转移，可水解探针测定等，通过允许使用更短的寡核苷酸，这些寡核苷酸具有更高的特异性和更好的鉴别能力。
• HYBRIDIZATION AND MISMATCH DISCRIMINATION USING OLIGONUCLEOTIDES CONJUGATED TO MINOR GROOVE BINDERS
  申请人：HEDGPETH JOEL

  公开号：US20090048427A1

  公开（公告）日：2009-02-19

  Conjugates between a minor groove binding molecule, such as the trimer of 1,2-dihydro-(3H)-pyrrolo[3,2-e]indole-7-carboxylate (CDPI 3 ), and an oligonucleotide form unusually stable hybrids with complementary target sequences, in which the tethered CDPI 3 group resides in the minor groove of the duplex. These conjugates can be used as probes and primers. Due to their unusually high binding affinity, conjugates as short as 8-mers can be used as amplification primers with high specificity and efficiency. MGB conjugation also increases the discriminatory power of short oligonucleotides, providing enhanced detection of nucleotide sequence mismatches by short oligonucleotides. The MGB-conjugated probes and primers described herein facilitate various analytic and diagnostic procedures, such as amplification reactions, PCR, detection of single-nucleotide polymorphisms, gene hunting, differential display, fluorescence energy transfer, hydrolyzable probe assays and others; by allowing the use of shorter oligonucleotides, which have higher specificity and better discriminatory power.

  一种小沟结合分子（例如1,2-二氢-（3H）-吡咯并[3,2-e]吲哚-7-羧酸三聚体（CDPI3））与寡核苷酸之间的共轭体形成与互补靶序列异常稳定的杂交体，在其中，被拴在一起的CDPI3基团位于双链的小沟中。这些共轭体可用作探针和引物。由于它们的结合亲和力异常高，因此即使是长度为8个碱基的共轭体也可用作具有高特异性和效率的扩增引物。MGB共轭还增加了短寡核苷酸的鉴别能力，通过短寡核苷酸提供了更好的核苷酸序列不匹配检测。所述的MGB共轭探针和引物可促进各种分析和诊断过程，例如扩增反应，PCR，单核苷酸多态性检测，基因寻找，差异显示，荧光能量转移，可水解探针测定等；通过使用更短的寡核苷酸，从而具有更高的特异性和更好的鉴别能力。
• COVALENTLY LINKED OLIGONUCLEOTIDE MINOR GROOVE BINDER CONJUGATES
  申请人：EPOCH PHARMACEUTICALS, INC.

  公开号：EP0819133A2

  公开（公告）日：1998-01-21
• US5801155A
  申请人：——

  公开号：US5801155A

  公开（公告）日：1998-09-01