2,2,2-Trifluoro-N-{2-[4-(2-hydroxyethyl)piperazin-1-yl]ethyl}acetamide | 87980-85-6

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 二嗪烷类
• 英文名称: 2,2,2-Trifluoro-N-{2-[4-(2-hydroxyethyl)piperazin-1-yl]ethyl}acetamide
• 英文别名: 2,2,2-trifluoro-N-[2-[4-(2-hydroxyethyl)piperazin-1-yl]ethyl]acetamide
• CAS: 87980-85-6
• 化学式: C₁₀H₁₈F₃N₃O₂
• 分子量: 269.26
• InChiKey: DJXYNLOZRRMURJ-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.2
• 重原子数：
  18
• 可旋转键数：
  5
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.9
• 拓扑面积：
  55.8
• 氢给体数：
  2
• 氢受体数：
  7

文献信息

• Specific binding assay method, reagent system and labelled conjugate for use in this method
  申请人：MILES LABORATORIES, INC.

  公开号：EP0087564A1

  公开（公告）日：1983-09-07

  An improved specific binding assay, e.g., homogeneous or heterogeneous immunoassay, for determining an analyte employing a labeled conjugate of said analyte and a photogenic, e.g., fluorescent, label. Where the analyte in the analyte-photophor conjugate whose light emission is measured in the assay as a function of the analyte is capable of significantly reducing such light emission upon coming into quenching orientation with the photophor, the present invention employs a labeled conjugate in which the analyte is joined to the photogenic label by a linking group which substantially hinders such quenching orientation. In this manner, ten percent or more of the photogenicity of the unconjugated photophor can be preserved in the measured analyte-photophor conjugate. The use of conventional flexible linking groups in the labeled conjugate allows quenching to occur with attendant large losses in assay sensitivity, whereas sensitivity is increased in the present invention by the use of relatively rigid linking groups. The invention is applicable to a wide variety of photogenic, e.g., fluorescent and chemiluminescent, binding assay techniques.

  一种改进的特异性结合测定法，例如均相或异相免疫测定法，用于测定一种分析物，该测定法采用了所述分析物与光致标签（例如荧光标签）的标记共轭物。如果分析物-光致抗蚀剂共轭物中的分析物在与光致抗蚀剂发生淬灭定向时，其发射的光作为分析物的函数被测量，而分析物-光致抗蚀剂共轭物中的分析物能够显著减少这种发射，则本发明采用一种标记的共轭物，其中分析物通过一个连接基团与光致标签连接，该连接基团基本上阻碍这种淬灭定向。通过这种方式，在测量的分析物-光致抗蚀剂共轭物中可以保留未共轭光致抗蚀剂百分之十或更多的光致性。在标记的共轭物中使用传统的柔性连接基团会导致淬灭现象的发生，随之而来的是检测灵敏度的大幅降低，而在本发明中使用相对刚性的连接基团则可提高灵敏度。本发明适用于各种光生（如荧光和化学发光）结合检测技术。