Unit Definition for 3'-5'-Phosphodiesterase: One unit will liberate 1.0 μmole of acid-soluble nucleotides from RNA per minute at pH 5.3 at 37 °C.
3'-Nucleotidase: One unit will hydrolyze 1.0 μ mole of orthophosphate from 3′-AMP per minute at pH 7.2 at 37 °C.