5-溴-4-氯-3-吲哚基磷酸酯对甲苯胺盐 | 6578-06-9

• 物质功能分类: 医药中间体 - 杂环化合物 - 吲哚类化合物
• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 有机磷酸及其衍生物
• 中文名称: 5-溴-4-氯-3-吲哚基磷酸酯对甲苯胺盐
• 中文别名: 对甲苯胺蓝/BCIP；5-溴-4-氯-3-吲哚基磷酸酯对甲苯胺盐（BCIP)；对甲苯胺蓝盐；对甲苯胺蓝；5-溴-4-氯-3-吲哚磷酸；5-溴-4-氯-3-吲哚基磷酸酯钾对甲苯胺盐；磷酸5-溴-4-氯-3-吲哚酯对甲苯胺盐；5-溴-4-氯-3-吲哚基磷酸盐；5-溴-4-氯-3-吲哚磷酸酯对甲苯胺盐；5-溴-4-氯-3-吲哚氧基磷酸酯对甲苯胺盐；5-溴-4-氯-3-吲哚磷酸对甲苯胺盐；磷酸 5-溴-4-氯-3-吲哚酯对甲苯胺盐；BCIP
• 英文名称: 5-bromo-4-chloro-3-indolyl phospate p-toluidine salt
• 英文别名: p-toluidine salt；(5-bromo-4-chloro-1H-indol-3-yl) hydrogen phosphate;(4-methylphenyl)azanium
• CAS: 6578-06-9
• 化学式: C₇H₉N*C₈H₆BrClNO₄P
• 分子量: 433.626
• InChiKey: QEIFSLUFHRCVQL-UHFFFAOYSA-N

物化性质

• 熔点：
  194-195℃
• 溶解度：
  DMF：20 mg/mL

计算性质

• 辛醇/水分配系数(LogP)：
  4.63
• 重原子数：
  24
• 可旋转键数：
  2
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.07
• 拓扑面积：
  109
• 氢给体数：
  4
• 氢受体数：
  5

安全信息

• 危险等级：
  6.1
• 危险品标志：
  Xi
• 危险类别码：
  R36/37/38
• WGK Germany：
  3
• 海关编码：
  29339190
• 安全说明：
  S26
• 危险性防范说明：
  P261,P280,P302+P352+P312,P304+P340+P312,P337+P313,P403+P233
• 危险性描述：
  H312,H315,H319,H335

制备方法与用途

生物活性

BCIP（BCIP对-甲基苯磺酸盐；X-磷酸酯对-甲基苯磺酸盐）是一种显色底物，用于通过比色法检测碱性磷酸酶活性。

反应信息

• 作为反应物：
  描述：

  5-溴-4-氯-3-吲哚基磷酸酯对甲苯胺盐 、 farnesyl phosphate bisammonium salt 在 吡啶 、 三乙胺 、 N,N'-羰基二咪唑 作用下， 以 N,N-二甲基甲酰胺 为溶剂， 反应 147.0h， 以41%的产率得到P1-farnesyl-P2-(5-bromo-4-chloro-3-indolyl) diphosphate monoammonium salt
  参考文献：
  名称：

  Convenient methods for the synthesis of P1-farnesyl-P2-indicator diphosphates

  摘要：

  To access P-1-farnesyl-P(2-)indicator diphosphates, more efficient methods for the synthesis of farnesyl-phosphate and diphosphates were developed. The procedures reported here provide more flexible conditions than the conventional imidazolide and morpholidate coupling methods. Milder conditions for the synthesis of sensitive allylic diphosphates and greatly improved reaction efficiencies provide access to novel reagents for analysis of diphosphate-based enzymatic reactions. (C) 2005 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmcl.2005.12.016

文献信息

• Methods and products related to the improved analysis of carbohydrates
  申请人：Bosques Carlos

  公开号：US20060127950A1

  公开（公告）日：2006-06-15

  The invention relates, in part, to the improved analysis of carbohydrates. In particular, the invention relates to the analysis of carbohydrates, such as N-glycans and O-glycans found on proteins and saccharides attached to lipids. Improved methods, therefore, for the study of glycosylation patterns on cells, tissue and body fluids are also provided. Information from the analysis of glycans, such as the glycosylation patterns on cells, tissues and in body fluids, can be used in diagnostic and treatment methods as well as for facilitating the study of the effects of glycosylation/altered glycosylation. Such methods are also provided. Methods are further provided to assess production processes, to assess the purity of samples containing glycoconjugates, and to select glycoconjugates with the desired glycosylation.

  本发明部分涉及改进的碳水化合物分析。具体而言，本发明涉及碳水化合物的分析，例如附着于脂质的蛋白质上的N-糖基和O-糖基。因此，还提供了改进的方法，用于研究细胞、组织和体液中的糖基化模式。从糖基分析中获得的信息，例如细胞、组织和体液中的糖基化模式，可用于诊断和治疗方法，以及促进糖基化/改变糖基化影响研究的方法。还提供了这样的方法。进一步提供了评估生产过程、评估含有糖联合物样品的纯度以及选择具有所需糖基化的糖联合物的方法。
• METHODS AND PRODUCTS RELATED TO THE IMPROVED ANALYSIS OF CARBOHYDRATES
  申请人：Gandhe Pankaj

  公开号：US20100136599A1

  公开（公告）日：2010-06-03

  The invention relates, in part, to the improved analysis of carbohydrates. In particular, the invention relates to the analysis of carbohydrates, such as N-glycans and O-glycans found on proteins. Improved methods, therefore, for the study of glycosylation patterns on cells, tissue and body fluids are also provided. Information regarding the analysis of glycans, such as the glycosylation patterns on cells, tissues and in body fluids, can be used in diagnostic and treatment methods as well as for facilitating the study of the effects of glycosylation/altered glycosylation on protein function. Such methods are also provided. Methods are also provided to assess protein production processes, to assess the purity of proteins produced, and to select proteins with the desired glycosylation.

  本发明涉及改进的碳水化合物分析方法。特别是，本发明涉及对蛋白质上发现的N-糖基和O-糖基等碳水化合物的分析。因此，还提供了用于研究细胞、组织和体液中糖基化模式的改进方法。有关糖基的分析信息，例如细胞、组织和体液中的糖基化模式，可用于诊断和治疗方法，以及促进研究糖基化/改变糖基化对蛋白质功能的影响。本发明还提供了这样的方法。还提供评估蛋白质生产过程、评估所生产蛋白质的纯度以及选择具有所需糖基化的蛋白质的方法。
• Detection method of abnormally responding lymphocytes as well as detection reagent and kit therefor
  申请人：JAPAN IMMUNO RESEARCH LABORATORIES CO., LTD.

  公开号：EP0304663A1

  公开（公告）日：1989-03-01

  Disclosed herein is a method for the detection of lymphocytes, responding to e.g., HIV-­infected T cells or cancer cells. An antibody, which can specifically recognize a saccharide chain represented by the following formula (I): is caused to act on a sample containing lymphocytes and lymphocytes conjugated with the antibody are then detected. Reagent and kit for the above detection are also disclosed.

  本发明公开了一种检测淋巴细胞的方法，这种淋巴细胞可与受艾滋病病毒感染的 T 细胞或癌细胞等反应。一种能特异性识别下式(I)代表的糖链的抗体作用于含有淋巴细胞的样品，然后检测与抗体结合的淋巴细胞。还公开了用于上述检测的试剂和试剂盒。
• Monoclonal antibodies against, and cell surface antigen of, lung carcinoma
  申请人：SANDOZ AG

  公开号：EP0356397A2

  公开（公告）日：1990-02-28

  Monoclonal antibodies and fragments thereof, hybrid cell lines producing these antibodies and methods of using them are described. They are characterized in that they - belong to Cluster w4 as defined at the International Workshop on SCC, London 1987, - show high capacity for in vivo localisation, - have low retention in non-target organs, - exhibit persistence at the target tumor and - bind to a glycoprotein antigen having the following characteristics: - molecular weight of approximately 45000 before and about 15000 to about 17000 after deglycosylation; - remains reactive with antibody SWA11 after deglycosylation; - remains present in cells after treatment with tunicamycin. They have an interesting pharmacological activity and may thus find application in the diagnosis and/or treatment of lung carcinoma.

  本文描述了单克隆抗体及其片段、产生这些抗体的杂交细胞系以及使用它们的方法。它们的特点是 - 属于 1987 年伦敦 SCC 国际研讨会定义的 w4 群、 - 体内定位能力强、 - 在非靶器官中的存留率低、 - 在靶肿瘤中表现出持久性 - 与具有以下特征的糖蛋白抗原结合： - 脱糖前分子量约为 45000，脱糖后分子量约为 15000 至 17000； - 脱糖后仍与抗体 SWA11 反应； - 用曲奈霉素处理后仍存在于细胞中。 它们具有有趣的药理活性，因此可用于诊断和/或治疗肺癌。
• Enzyme assays
  申请人：Sankyo Company Limited

  公开号：EP0476930A1

  公开（公告）日：1992-03-25

  Accurate enzyme assays suitable for use in immunoassay techniques are now possible for enzymes associated with the dimerisation of indoxyl esters to form the corresponding indigo, the assays utilising the production of hydrogen peroxide from this reaction, which is found to be sufficiently consistent that it can be used to accurately calculate enzyme activity down to levels of 1 in 1000 compared to colorimetric techniques for the same enzymes.

  对于与吲哚基酯二聚形成相应的靛蓝有关的酶，现在可以进行适用于免疫测定技术的精确酶测定，这种测定利用了从这一反应中产生的过氧化氢。

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