[(2S,5R)-5-(2-amino-6,8-dioxo-1,7-dihydropurin-9-yl)oxolan-2-yl]methyl dihydrogen phosphate

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘌呤核苷酸
• 英文名称: [(2S,5R)-5-(2-amino-6,8-dioxo-1,7-dihydropurin-9-yl)oxolan-2-yl]methyl dihydrogen phosphate
• 化学式: C₁₀H₁₄N₅O₇P
• 分子量: 347.22
• InChiKey: YCKAFYONYFFORQ-CRCLSJGQSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -3.3
• 重原子数：
  23
• 可旋转键数：
  4
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  176
• 氢给体数：
  5
• 氢受体数：
  7

文献信息

• Method for nucleic acid detection by guiding through a nanopore
  申请人：Oxford Nanopore Technologies Limited

  公开号：US11085078B2

  公开（公告）日：2021-08-10

  The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore; (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.

  本发明提供了一种检测样品中目标多核苷酸的方法，包括(a) 将样品与与靶多核苷酸中的序列结合的引导多核苷酸和多核苷酸引导的效应蛋白接触，其中引导多核苷酸和多核苷酸引导的效应蛋白与样品中存在的任何靶多核苷酸形成复合物；(b) 将样品与包含跨膜孔的膜接触； (c) 向膜施加电位；以及 (d) 监测复合物与跨膜孔相互作用产生的效应的存在与否，以确定复合物的存在与否，从而检测样品中的靶多核苷酸。
• Method
  申请人：Oxford Nanopore Technologies PLC

  公开号：US11390910B2

  公开（公告）日：2022-07-19

  A method for determining the presence, absence or amount of two or more target polynucleotides in a sample comprising additional components, the method comprising: (i) contacting the sample with a panel of two or more probes under conditions suitable for hybridisation of the target polynucleotides to the probes, wherein: (a) each probe comprises a non-hybridisation region and a hybridisation region that specifically hybridises to one of the target polynucleotides to form a hybridised probe; and (b) the hybridisation region of a probe of the panel comprises one or more non-natural nucleotides; (ii) contacting the sample prepared in step (i) with a transmembrane pore through which a single stranded polynucleotide but not a double stranded polynucleotide can pass and applying a potential difference to the transmembrane pore such that the hybridised probes in the sample interact with the pore; (iii) measuring current blockades having a duration within a defined window, wherein: (a) the one or more non-natural nucleotides present in the hybridisation region of the probe increase or decrease the duration of the current blockade due to the probe hybridised to its target polynucleotide such that the proportion of current blockades that occur within the window due to the interaction of the hybridised probes with the pore is increased compared to when the corresponding one or more natural nucleotides are present in the hybridisation region; and (b) each hybridised probe gives rise to a current blockade indicative of that probe; and (iv) correlating the measured current blockades with the probes, thereby determining the presence, absence or amount of the two or more target polynucleotides in the sample.

  一种用于确定包含附加成分的样品中存在、不存在两种或多种目标多核苷酸或其数量的方法，该方法包括：(i) 在适合目标多核苷酸与探针杂交的条件下，将样品与两种或多种探针组成的小组接触，其中：(a) 每个探针包括一个非杂交区和一个杂交区，该杂交区与目标多核苷酸之一特异性杂交以形成杂交探针；以及 (b) 组合探针的杂交区包括一个或多个非天然核苷酸；(ii) 将步骤(i)中制备的样品与单链多核苷酸而非双链多核苷酸可以通过的跨膜孔隙接触，并对跨膜孔隙施加电位差，使样品中的杂交探针与孔隙相互作用； (iii) 测量在规定窗口内持续时间的电流阻断，其中：(a) 存在于探针杂交区域中的一个或多个非天然核苷酸增加或减少了探针与其目标多核苷酸杂交导致的电流阻断持续时间，从而与相应的一个或多个天然核苷酸存在于杂交区域中时相比，由于杂交探针与孔相互作用而在窗口内发生的电流阻断比例增加；(iv) 将测得的电流阻滞与探针相关联，从而确定样品中是否存在两种或多种目标多核苷酸或其数量。
• QUADRUPLEX METHOD
  申请人：Oxford Nanopore Technologies Limited

  公开号：EP2917366A1

  公开（公告）日：2015-09-16
• METHOD
  申请人：Oxford Nanopore Technologies plc

  公开号：EP3464616B1

  公开（公告）日：2022-05-04
• METHOD FOR DETERMINING THE PRESENCE, ABSENCE OR AMOUNT OF TWO OR MORE TARGET POLYNUCLEOTIDES
  申请人：Oxford Nanopore Technologies plc

  公开号：EP3529373B1

  公开（公告）日：2022-12-07