[[3-Amino-5-(2,6-diaminopurin-9-yl)-4-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [5-(3-carbamoylphenyl)-3,4-dihydroxyoxolan-2-yl]methyl hydrogen phosphate

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘌呤核苷酸
• 英文名称: [[3-Amino-5-(2,6-diaminopurin-9-yl)-4-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [5-(3-carbamoylphenyl)-3,4-dihydroxyoxolan-2-yl]methyl hydrogen phosphate
• 英文别名: [[3-amino-5-(2,6-diaminopurin-9-yl)-4-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [5-(3-carbamoylphenyl)-3,4-dihydroxyoxolan-2-yl]methyl hydrogen phosphate
• 化学式: C₂₂H₃₀N₈O₁₃P₂
• 分子量: 676.5
• InChiKey: DETFYNOUEPRTFS-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -8.1
• 重原子数：
  45
• 可旋转键数：
  11
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.45
• 拓扑面积：
  346
• 氢给体数：
  9
• 氢受体数：
  19

文献信息

• [EN] CHEMICALLY MODIFIED LIGASE COFACTORS, DONORS AND ACCEPTORS<br/>[FR] COFACTEURS, DONNEURS ET ACCEPTEURS DE LIGASE MODIFIÉS CHIMIQUEMENT
  申请人：TRILINK BIOTECHNOLOGIES INC

  公开号：WO2014144979A1

  公开（公告）日：2014-09-18

  Provided herein are methods for ligation of polynucleotides containing modified ligation components, particularly modified ligase cofactors, modified acceptors and modified donors. The methods readily applied to ligation-based assays for detection of a nucleic acid sequence where the use of the modified cofactor improves discrimination between matched and mismatched templates. Furthermore, the use of the modified ligation components reduces or eliminates the ligation in the absence of nucleic acid template. In addition, methods are applied to the preparation of nucleic acid libraries using modified acceptor probes and modified donor probes that reduce or eliminate probe dimerization during the ligation process.