Sulfosuccinimidyl 6-(biotinamido)hexanoate

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 生物素及其衍生物
• 英文名称: Sulfosuccinimidyl 6-(biotinamido)hexanoate
• 英文别名: 2,5-dioxo-1-[6-[5-(2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl)pentanoylamino]hexanoyloxy]pyrrolidine-3-sulfonic acid
• 化学式: C₂₀H₃₀N₄O₉S₂
• 分子量: 534.612
• InChiKey: UQZHJQWIISKTJN-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.3
• 重原子数：
  35
• 可旋转键数：
  14
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.75
• 拓扑面积：
  222
• 氢给体数：
  4
• 氢受体数：
  10

反应信息

• 作为反应物：
  描述：

  、 Sulfosuccinimidyl 6-(biotinamido)hexanoate 在 碳酸氢钠 作用下， 反应 24.0h， 以72%的产率得到
  参考文献：
  名称：

  Synthesis of CMP-9″-modified-sialic acids as donor substrate analogues for mammalian and bacterial sialyltransferases

  摘要：

  Cytidine-5'-monophospho-sialic acid (CMP-Neu5Ac) derivatives bearing a phenyl group in which the tether length between the phenyl group and the 9-position of Neu5Ac varied were synthesized and evaluated as substrates for sialyltransferases. In the synthesis of the compounds, a coupling reaction between methyl 5-acetamido-4,7,8-tri-O-acetyl-9-azido-3,5,9-trideoxy-beta-D-glycero-D-galacto-2-nonulopyranosonate and 2-cyanoethyl 2',3'-O,N(4), triacetylcytidine-5'-yl N,N-diisopropylphosphoramidite was carried out and the phosphite derivative thus obtained was oxidized and then deprotected to yield CMP-9"-azido-Neu5Ac. Modification of the 9-amino group prepared by reduction of the azido groups was performed by the use of several phenyl-substituted alkylcarboxylic acid derivatives. Using these CMP-9"-modified-Neu5Ac analogues bearing the phenyl-substituted alkyl-amide group, sialyltransferase assays were performed with both rat liver alpha-(2,6)-sialyltransferase and Photobacterium alpha-(2 -> 6)-sialyltransferase. These 9-modified analogues could be transferred to disaccharide acceptors, and a practical enzymatic synthesis using CMP-9"-modified-Neu5Ac yielded sialoside analogues and sialylglycoproteins in good yield. These experiments demonstrate that the Photobacterium sialyltransferase can be used in the synthesis of sialoside analogues having a large substituent at the 9-position of Neu5Ac. (c) 2007 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.carres.2007.05.029

文献信息

• Haellgren, Christer; Hindsgaul, Ole, Journal of Carbohydrate Chemistry, 1995, vol. 14, # 4/5, p. 453 - 464
  作者：Haellgren, Christer、Hindsgaul, Ole

  DOI：——

  日期：——
• SITE SPECIFIC PROTEIN MODIFICATIONS
  申请人：Novartis AG

  公开号：EP3157942B1

  公开（公告）日：2020-06-17
• METHODS FOR DETECTION OF CYCLOSPORIN A
  申请人：Zheng Yi Feng

  公开号：US20090170218A1

  公开（公告）日：2009-07-02

  Methods and reagents are disclosed for determining the presence and/or amount of cyclosporin A in a medium suspected of containing cyclosporin A. In the method a combination is provided in a medium. The combination comprises (i) the sample, (ii) a first member of a signal producing system (sps) associated with a first support wherein the first sps member is capable of activating a second member of the sps and wherein the first support is associated with a first member of a specific binding pair, and (iii) the second sps member associated with a second support wherein the second sps member is activatable by the first sps member. The second support comprises either (I) cyclosporin C or cyclosporin A and the combination further comprises a conjugate of an antibody for cyclosporin A and a second member of the specific binding pair or (II) antibody for cyclosporin A and the combination further comprises a conjugate of cyclosporin A and a second member of the specific binding pair. The combination is subjected to conditions for binding of cyclosporin A to the antibody for cyclosporin A. The first sps member is activated and the amount of signal generated by the second sps member is detected. The amount of signal is related to the presence and/or amount of cyclosporin A in the sample.
• One-Step Enzymatic and Amine Detection Technique
  申请人：Song Xuedong

  公开号：US20090226938A1

  公开（公告）日：2009-09-10

  A technique for detecting the presence or quantity of an enzyme (or enzyme inhibitor) and/or an amine within a test sample is provided. For example, in one embodiment, a diagnostic test kit is employed that utilizes reactive complexes that each includes a substrate joined (e.g., covalently bonded, physically adsorbed, etc.) to a reporter and a separation species. Upon contacting the reactive complexes, enzymes may cleave the substrate and release the reporter. Moreover, the test kit may also employ a chemichromic dye, i.e., a dye that exhibits a detectable color change upon chemical reaction with one or more functional groups, such as amino groups. The signal generated (directly or indirectly) by the reporter and chemichromic dye may then be used to indicate the presence or quantity of an enzyme (or enzyme inhibitor) and amine, respectively, within the test sample.
• BIOTIN-AVIDIN CONTROLLED DELIVERY SYSTEMS
  申请人：DREXEL UNIVERSITY

  公开号：US20170112971A1

  公开（公告）日：2017-04-27

  The application relates to the delivery of immunomodulatory molecules, including cytokines, to the situs of tissue scaffolds, and wounds including injuries.