N-acetyllactosaminide 3-alpha-galactosyltransferase | 306996-46-3

• 分子结构分类: 转移酶 - 糖基转移酶 - 己糖基转移酶
• 英文名称: N-acetyllactosaminide 3-alpha-galactosyltransferase
• 英文别名: alpha-galactosyltransferase；UDP-Gal:beta-D-Gal(1,4)-D-GlcNAc alpha(1,3)-galactosyltransferase；UDP-Gal:N-acetyllactosaminide alpha(1,3)-galactosyltransferase；UDP-Gal:N-acetyllactosaminide alpha-1,3-D-galactosyltransferase；UDP-Gal:Galbeta1->4GlcNAc-R alpha1->3-galactosyltransferase；UDP-galactose-acetyllactosamine alpha-D-galactosyltransferase；UDPgalactose:beta-D-galactosyl-beta-1,4-N-acetyl-D-glucosaminyl-glycopeptide alpha-1,3-D-galactosyltransferase；glucosaminylglycopeptide alpha-1,3-galactosyltransferase；uridine diphosphogalactose-acetyllactosamine alpha1->3-galactosyltransferase；uridine diphosphogalactose-acetyllactosamine galactosyltransferase；uridine diphosphogalactose-galactosylacetylglucosaminylgalactosylglucosylceramide galactosyltransferase；beta-D-galactosyl-N-acetylglucosaminylglycopeptide alpha-1,3-galactosyltransferase；UDP-galactose:N-acetyllactosaminide 3-alpha-D-galactosyltransferase；UDP-galactose:beta-D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-R 3-alpha-D-galactosyltransferase；UDP-galactose:beta-D-galactosyl-(1->4)-beta-N-acetyl-D-glucosaminyl-R 3-alpha-D-galactosyltransferase；E317A-mutant of bovine α3-galactosyltransferase
• CAS: 306996-46-3；78642-28-1；96477-57-5；128449-51-4

反应信息

• 作为试剂：
  描述：

  尿苷 5’-二二氧磷基半乳糖二钠盐 在 N-acetyllactosaminide 3-alpha-galactosyltransferase 苯磺酰胺 、 sodium azide 、 manganese(ll) chloride 作用下， 以 various solvent(s) 为溶剂， 生成 1-叠氮-1-脱氧-β-D-吡喃半乳糖苷
  参考文献：
  名称：

  Chemical Rescue of α3-Galactosyltransferase. Implications in the Mechanism of Retaining Glycosyltransferases

  摘要：

  The mechanism of retaining glycosyltransferases is still poorly understood and the subject of current debate. Both double displacement and front side single displacement (SNi) mechanisms have been proposed. A "chemical rescue methodology" is here applied to a retaining alpha3-galactosyltransferase. Azide as exogenous nucleophile rescues the activity of the inactive E317A mutant to give beta-d-galactosylazide. This result fits best with a double displacement mechanism in which Glu317 is the enzyme nucleophile involved in the formation of a glycosyl-enzyme intermediate.

  DOI：

  10.1021/ja0659931