nicotinamide adenine dinucleotide phosphate | 604-79-5

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - （5'->5'）-二核苷酸
• 英文名称: nicotinamide adenine dinucleotide phosphate
• 英文别名: NADP+；NADPH；β-nicotinamide adenine dinucleotide phosphate；NAD(+)；triphosphopyridine nucleotide；β-nicotinamide adenine dinucleotide phosphate, oxidized form；Nicotinamid-adenin-dinucleotid-phosphat；β-NADP+；NADP nicotinamide-adenine-dinucleotide phosphate；[[(2R,3R,4R,5R)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2R,3S,4R,5R)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl hydrogen phosphate
• CAS: 604-79-5
• 化学式: C₂₁H₂₉N₇O₁₇P₃
• 分子量: 744.419
• InChiKey: XJLXINKUBYWONI-NNYOXOHSSA-O

物化性质

• 碰撞截面：
  230.9 Ų [M+H]+ [CCS Type: DT, Method: single field calibrated with Agilent tune mix (Agilent)]

反应信息

• 作为反应物：
  描述：

  葡萄糖 、 nicotinamide adenine dinucleotide phosphate 生成 葡糖酸
  参考文献：
  名称：

  METHOD FOR PRODUCING ASCORBIC ACID INTERMEDIATES

  摘要：

  本发明涉及非发酵方法用于生产ASA中间体，KDG，DKG和KLG以及辅因子再生方法。该发明提供了包含异源核酸编码酶的基因工程宿主细胞，这些酶在该过程中非常有用。

  公开号：

  US20020177198A1
• 作为产物：
  描述：

  还原型辅酶Ⅰ 、 nicotinamide adenine dinucleotide 、 反应 0.37h， 生成 还原型辅酶II(NADPH)四钠盐 、 nicotinamide adenine dinucleotide phosphate
  参考文献：
  名称：

  Method for highly sensitive determination of NADH using kinase

  摘要：

  本发明涉及一种利用循环反应，通过使用特异于NADH的NADH激酶确定溶液中微量NADH或XTP的方法，具有高灵敏度，该方法允许高灵敏度地确定NADH而不受NAD.sup.+的影响，因此对于疾病诊断等方面具有实用价值。

  公开号：

  US05250416A1
• 作为试剂：
  描述：

  2-苄基丙烯醛 在 葡萄糖 、 还原型辅酶Ⅰ 、 nicotinamide adenine dinucleotide phosphate 作用下， 以 磷酸肌酸 、 乙酸乙酯 为溶剂， 反应 2.0h， 以As a result, (R)-2-methyl-3-phenylpropanal with the optical purity of 94.7% ee was obtained at the reaction conversion rate of 99.3%的产率得到(2R)-2-methyl-3-phenylpropanal
  参考文献：
  名称：

  PROCESS FOR PRODUCTION OF OPTICALLY ACTIVE 2-SUBSTITUTED PROPANAL DERIVATIVE

  摘要：

  本发明涉及一种制备光学活性2-取代丙醛衍生物的方法，更具体地说，涉及一种通过使用一种能够立体选择性还原所述碳-碳双键的酶源，立体选择性地还原2-取代丙烯醛衍生物的碳-碳双键，从廉价且易得材料中方便地制备光学活性2-取代丙醛衍生物，特别是光学活性2-烷基丙醛衍生物，该衍生物在制药产品、甜味剂等中间体方面具有应用价值。

  公开号：

  US20100003732A1

文献信息

• Mutated 6-phosphogluconate dehydrogenase
  申请人：Ikeda Masato

  公开号：US20050079586A1

  公开（公告）日：2005-04-14

  The present invention relates to a polypeptide having a modified amino acid sequence of 6-phosphogluconate dehydrogenase (hereinafter abbreviated as GND) derived from a microorganism belonging to the genus Corynebacterium , said modification being substitution of the amino acid residue(s) at the position(s) corresponding to the 158th and/or the 361st amino acid(s) of the amino acid sequence shown in SEQ ID NO: 1, and having GND activity; DNA encoding the polypeptide; a recombinant DNA comprising the DNA; a transformant carrying the recombinant DNA; a microorganism carrying the DNA on the chromosome; and a process for producing a useful substance which comprises culturing the transformant or the microorganism in a medium.

  本发明涉及一种多肽，其具有来自属于科林氏杆菌属的微生物的6-磷酸葡糖酸脱氢酶（以下简称GND）的修改氨基酸序列，该修改是替换与SEQ ID NO: 1中显示的氨基酸序列的第158和/或第361个氨基酸位置相对应的氨基酸残基，并具有GND活性；编码该多肽的DNA；包含该DNA的重组DNA；携带重组DNA的转化体；携带DNA的染色体的微生物；以及生产有用物质的方法，其中包括在培养基中培养转化体或微生物。
• NON-LETHAL METHODS FOR CONDITIONING A RECIPIENT FOR BONE MARROW TRANSPLANTATION
  申请人：——

  公开号：US20010009663A1

  公开（公告）日：2001-07-26

  The present invention relates to non-lethal methods of conditioning a recipient for bone marrow transplantation. In particular, it relates to the use of nonlethal doses of total body irradiation, total lymphoid irradiation, cell type-specific or cell marker-specific antibodies, especially antibodies directed to bone marrow stromal cell markers or the CD8 cell marker, cytotoxic drugs, or a combination thereof. The methods of the invention have a wide range of applications, including, but not limited to, the conditioning of an individual for hematopoietic reconstitution by bone marrow transplantation for the treatment of hematologic malignancies, hematologic disorders, autoimmunity, infectious diseases such as acquired immunodeficiency syndrome, and the engraftment of bone marrow cells to induce tolerance for solid organ, tissue and cellular transplantation.

  本发明涉及一种非致命的方法，用于为骨髓移植接受者进行调理。特别是，涉及使用非致命剂量的全身照射、全淋巴照射、细胞类型特异性或细胞标记特异性抗体，特别是针对骨髓基质细胞标记或CD8细胞标记的抗体、细胞毒药物或其组合物。本发明的方法具有广泛的应用，包括但不限于通过骨髓移植进行造血重建治疗血液恶性肿瘤、血液疾病、自身免疫性疾病、获得性免疫缺陷综合症等感染性疾病，以及移植固体器官、组织和细胞的骨髓细胞移植诱导耐受性。
• High throughput method for functionally classifying proteins identified using a genomics approach
  申请人：——

  公开号：US20020168686A1

  公开（公告）日：2002-11-14

  The present invention provides a method for functionally classifying a protein that is capable of unfolding due to a thermal change. The method comprises screening one or more of a multiplicity of different molecules for their ability to shift the thermal unfolding curve of the protein, wherein a shift in the thermal unfolding curve indicates that the molecule binds to the protein or affects the stability in a measurable way; generating an activity spectrum for the protein wherein the activity spectrum reflects a set of molecules, from the multiplicity of molecules, that shift the thermal unfolding curve, of the protein and therefore are ligands that bind to the protein, comparing the activity spectrum for the protein to one or more functional reference spectrum lists; and classifying the protein according to the set of molecules in the multiplicity of different molecules that shift the thermal unfolding curve of the protein.

  本发明提供了一种功能分类能够由于热变化而发生展开的蛋白质的方法。该方法包括筛选一种或多种不同分子的多重组合，以检测它们移动蛋白质的热展开曲线的能力，其中热展开曲线的移动表示该分子结合到蛋白质或以可测量的方式影响其稳定性；生成蛋白质的活性谱，其中活性谱反映了从多重分子中移动蛋白质的热展开曲线的分子集，因此是结合到蛋白质的配体；将蛋白质的活性谱与一个或多个功能参考谱列表进行比较；并根据多种不同分子的集合将蛋白质进行分类，这些分子移动蛋白质的热展开曲线。
• Methods for producing hydroxy amino acids and derivatives thereof
  申请人：Rozzell David J.

  公开号：US20050100993A1

  公开（公告）日：2005-05-12

  Hydroxy-amino acids are provided and are prepared by contacting a substituted P-ketodiester having a ketone group and two ester functional groups with a ketoreductase under conditions permitting the reduction of the keytone group to an alcohol. Only one of the ester functional groups is regioselectively hydrolyzed to the corresponding carboxylic acid, whereby a non-hydrolyzed ester functional group remains. The carboxylic acid is converted to an amine to produce a hydroxy-amino acid.

  提供了羟基氨基酸，并通过将具有酮基和两个酯官能团的取代P-酮二酯与酮还原酶接触，在条件允许酮基还原为醇的情况下制备。仅选择性水解其中一个酯官能团为相应的羧酸，从而保留未水解的酯官能团。将羧酸转化为胺以产生羟基氨基酸。
• Novel carbonyl reductase, gene thereof and method of using the same
  申请人：——

  公开号：US20030186412A1

  公开（公告）日：2003-10-02

  The present invention provides a novel polypeptide producing (S)-N-benzyl-3-pyrrolidinol, a DNA coding for it and a method of using them. A polypeptide having the following physicochemical properties (1) to (5): (1) Action: It asymmetrically reduces N-benzyl-3-pyrrolidinone to produce (S)-N-benzyl-3-pyrrolidinol with NADPH as a coenzyme; (2) Optimum action pH: 4.5 to 5.5; (3) Optimum action temperature: 40° C. to 45° C.; (4) Molecular weight: About 29,000 as determined by gel filtration analysis, about 35,000 as determined by SDS-polyacrylamide gel electrophoresis analysis; (5) Inhibitor: It is inhibited by the divalent copper ion. Further, a polypeptide having the amino acid sequence shown under SEQ ID NO:1 in the sequence listing; or a polypeptide having an amino acid sequence obtainable from the amino acid sequence shown under SEQ ID NO:1 in the sequence listing by substitution, insertion, deletion and/or addition of one or more amino acids and having enzyme activity in asymmetrically reducing N-benzyl-3-pyrrolidinone to produce (S)-N-benzyl-3-pyrrolidinol.

  本发明提供了一种新型的多肽产生(S)-N-苄基-3-吡咯醇，一种编码它的DNA以及使用它们的方法。该多肽具有以下物理化学性质(1)到(5)： (1)作用：使用NADPH作为辅酶，不对称还原N-苄基-3-吡咯酮以产生(S)-N-苄基-3-吡咯醇； (2)最适作用pH：4.5到5.5； (3)最适作用温度：40℃到45℃； (4)分子量：通过凝胶过滤分析确定约为29,000，通过SDS-聚丙烯酰胺凝胶电泳分析确定约为35,000； (5)抑制剂：受到二价铜离子的抑制。 此外，本发明还提供了具有序列列表中SEQ ID NO:1所示氨基酸序列的多肽；或者通过替换、插入、删除和/或添加一个或多个氨基酸获得的具有不对称还原N-苄基-3-吡咯酮以产生(S)-N-苄基-3-吡咯醇酶活性的氨基酸序列。