5'-Deoxy-5'-Iodocytidin | 51392-09-7

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 5'-脱氧核糖核苷
• 英文名称: 5'-Deoxy-5'-Iodocytidin
• 英文别名: 5'-iodo-5'-deoxy-cytidine；5'-Deoxy-5'-Iodocytidine；4-amino-1-[(2R,3R,4S,5S)-3,4-dihydroxy-5-(iodomethyl)oxolan-2-yl]pyrimidin-2-one
• CAS: 51392-09-7
• 化学式: C₉H₁₂IN₃O₄
• 分子量: 353.116
• InChiKey: ZFSSJWRSRMQVTI-XVFCMESISA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.5
• 重原子数：
  17
• 可旋转键数：
  2
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.56
• 拓扑面积：
  108
• 氢给体数：
  3
• 氢受体数：
  4

反应信息

• 作为反应物：
  描述：

  2-乙酰氧基异丁酰氯 、 5'-Deoxy-5'-Iodocytidin 以 乙腈 为溶剂， 生成 3c-acetoxy-2t-iodomethyl-(3ar,9ac)-2,3,3a,9a-tetrahydro-furo[2',3':4,5]oxazolo[3,2-a]pyrimidin-6-ylideneamine
  参考文献：
  名称：

  2-酰氧基异丁酰卤化物与核苷的反应。V.与胞苷及其衍生物的反应；

  摘要：

  DOI：

  10.1021/jo00929a009

文献信息

• Detection of nucleic acid targets using chemically reactive oligonucleotide probes
  申请人：Dxterity Diagnostics

  公开号：EP2677039A2

  公开（公告）日：2013-12-25

  The present invention provides compositions, apparatuses and methods for detecting one or more nucleic acid targets present in a sample. Methods of the invention include utilizing two or more oligonucleotide probes that reversibly bind a target nucleic acid in close proximity to each other and possess complementary reactive ligation moieties. When such probes have bound to the target in the proper orientation, they are able to undergo a spontaneous chemical ligation reaction that yields a ligated oligonucleotide product. In accordance with the invention, the presence of the target(s) of interest can be determined by measuring the presence or amount of ligated oligonucleotide product.

  本发明提供了用于检测样品中存在的一种或多种核酸靶标的组合物、装置和方法。本发明的方法包括利用两个或多个寡核苷酸探针，这些探针可逆地与靶核酸紧密结合，并具有互补的反应性连接分子。当这些探针以适当的方向与靶核结合后，就能发生自发的化学连接反应，产生连接的寡核苷酸产物。根据本发明，可以通过测量连接寡核苷酸产物的存在或数量来确定是否存在感兴趣的靶标。
• Chemical ligation dependent probe amplification (CLPA)
  申请人：DXTerity Diagnostics Incorporated

  公开号：EP2765205A1

  公开（公告）日：2014-08-13

  The present invention provides compositions, apparatuses and methods for detecting one or more nucleic acid targets present in a sample. Methods of the invention include utilizing two or more oligonucleotide probes that reversibly bind a target nucleic acid in close proximity to each other and possess complementary reactive ligation moieties. When such probes have bound to the target in the proper orientation, they are able to undergo a spontaneous chemical ligation reaction that yields a ligated oligonucleotide product. In one aspect, the ligation product is of variable length that correlates with a particular target. Following chemical ligation, the probes may be amplified and detected by capillary electrophoresis or microarray analysis.

  本发明提供了用于检测样品中存在的一种或多种核酸靶标的组合物、装置和方法。本发明的方法包括利用两个或多个寡核苷酸探针，这些探针可逆地与靶核酸紧密结合，并具有互补的反应性连接分子。当这些探针以适当的方向与靶核结合后，它们就能发生自发的化学连接反应，产生连接的寡核苷酸产物。在一个方面，连接产物的长度是可变的，与特定的靶标相关。化学连接后，探针可通过毛细管电泳或芯片分析进行扩增和检测。
• Methods and compositions for detecting target nucleic acids
  申请人：Terbrueggen Robert

  公开号：US10066257B2

  公开（公告）日：2018-09-04

  The present invention provides compositions, apparatuses and methods for detecting one or more nucleic acid targets present in a sample. Methods of the invention include utilizing two or more ligation probes that reversibly bind a target nucleic acid in close proximity to each other and possess complementary reactive ligation moieties. When such probes have bound to the target in the proper orientation, they are able to undergo a spontaneous chemical ligation reaction that yields a ligation product that is directly detected or that is amplified to produce amplicons that are then detected. The present invention also provides methods to stabilize sample RNA so that degradation does not significantly affect the results of the analysis.

  本发明提供了用于检测样品中存在的一种或多种核酸靶标的组合物、装置和方法。本发明的方法包括利用两个或多个可逆结合靶核酸的连接探针，这些探针相互靠近并具有互补的反应性连接分子。当这些探针以适当的方向与靶核酸结合后，它们就能发生自发的化学连接反应，产生可直接检测的连接产物，或经扩增产生可检测的扩增子。本发明还提供了稳定样本 RNA 的方法，使降解不会对分析结果产生重大影响。
• Compositions and methods for nonenzymatic ligation of oligonucleotides and detection of genetic polymorphisms
  申请人：Kool T. Eric

  公开号：US20060160125A1

  公开（公告）日：2006-07-20

  The invention is directed to novel compositions and methods for nonenzymatic ligation of oligonucleotides. In one aspect of the invention, the nonenzymatic ligation is selenium-mediated or tellurium mediated ligation. In another aspect, the invention provides for the use of fluorescence resonance energy transfer (FRET) to detect the nonenzymatic ligation.

  本发明涉及寡核苷酸非酶连接的新型组合物和方法。在本发明的一个方面，非酶连接是硒介导或碲介导的连接。在另一方面，本发明提供了使用荧光共振能量转移（FRET）检测非酶连接的方法。
• DETECTION OF NUCLEIC ACID TARGETS USING CHEMICALLY REACTIVE OLIGONUCLEOTIDE PROBES
  申请人：Dxterity Diagnostics

  公开号：EP2021514A2

  公开（公告）日：2009-02-11