[3-(1-氯-3'-甲氧基螺[金刚烷-4,4'-二氧杂环丁烷]-3'-基)苯基]磷酸二氢酯 | 142456-88-0

• 物质功能分类: 医药中间体 - 原料药中间体
• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 有机磷酸及其衍生物
• 中文名称: [3-(1-氯-3'-甲氧基螺[金刚烷-4,4'-二氧杂环丁烷]-3'-基)苯基]磷酸二氢酯
• 中文别名: 4-氯-2-(3-硝基苯基)-1H-咪唑并[4,5-c]吡啶
• 英文名称: Cspd
• 英文别名: [3-(1-chloro-3'-methoxyspiro[adamantane-4,4'-dioxetane]-3'-yl)phenyl] dihydrogen phosphate
• CAS: 142456-88-0
• 化学式: C₁₈H₂₂ClO₇P
• 分子量: 416.8
• InChiKey: QWXOJIDBSHLIFI-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.8
• 重原子数：
  27
• 可旋转键数：
  4
• 环数：
  6.0
• sp3杂化的碳原子比例：
  0.67
• 拓扑面积：
  94.4
• 氢给体数：
  2
• 氢受体数：
  7

制备方法与用途

简介

CSPD是化学发光的碱性磷酸酶底物，适用于蛋白质或核酸在硝酸纤维素膜上的印迹。此外，它也可用于基于溶液的测定。CSPD化学发光底物以其出色的灵敏度、速度和易用性检测碱性磷酸酶及其标记分子。相较于传统荧光底物甲基伞形酮磷酸酯 (MUP) 和比色底物对硝基苯磷酸盐 (pNPP)，它提供了更高的灵敏度。低背景发光与高强度光输出相结合，使碱性磷酸酶标记的物质能够以尽可能高的灵敏度和信噪比进行检测。

这种多功能的化学发光底物在基于膜的应用（例如 Southern、Northern 和 Western 印迹）中表现出高灵敏度。同时，CSPD 也可用于基于溶液的试验，如免疫检测、DNA 探针试验、酶试验和报告基因检测。约10分钟内达到最大光照水平，并可持续辉光发射数小时。对于溶液测定，需要化学发光增强剂以提高光输出和灵敏度；而基于膜的测定可能也需要该试剂来提升效果。

用途

碱性磷酸酶的化学发光底物—CSPD作为一种最新的超灵敏底物，反应速度快，在短时间内提供可靠的结果。它同样能够保证出色的灵敏度、速度及易用性，使用户能够检测到碱性磷酸酶及其标记分子。

文献信息

• NUCLEIC ACID AMPLIFICATION
  申请人：Bornarth Carole

  公开号：US20080128298A1

  公开（公告）日：2008-06-05

  Disclosed are compositions and methods for amplification of nucleic acid sequences of interest. It has been discovered that amplification reactions can produce amplification products of high quality, such as low amplification bias, if performed on an amount of nucleic acid at or over a threshold amount and/or on nucleic acids at or below a threshold concentration. The threshold amount and concentration can vary depending on the nature and source of the nucleic acids to be amplified and the type of amplification reaction employed. Disclosed is a method of determining the threshold amount and/or threshold concentration of nucleic acids that can be used with nucleic acid samples of interest in amplification reactions of interest. Because amplification reactions can produce high quality amplification products, such as low bias amplification products, below the threshold amount and/or concentration of nucleic acid, such below-threshold amounts and/or concentrations can be used in amplification reactions.

  本发明涉及一种扩增感兴趣的核酸序列的组合物和方法。已经发现，如果在等于或超过阈值数量的核酸或低于阈值浓度的核酸上进行扩增反应，则可以产生高质量的扩增产物，例如低扩增偏差。阈值数量和浓度取决于要扩增的核酸的性质和来源以及所采用的扩增反应类型。本发明还涉及一种确定可用于扩增感兴趣的核酸样品的阈值数量和/或阈值浓度的方法。由于扩增反应可以产生高质量的扩增产物，例如低偏差扩增产物，因此可以在低于阈值数量和/或浓度的核酸中使用这些低于阈值的数量和/或浓度进行扩增反应。
• Luminescent labeled immunoassay method and analysis element therefor
  申请人：FUJI PHOTO FILM CO., LTD.

  公开号：EP1045248A2

  公开（公告）日：2000-10-18

  A liquid sample containing an analyte antibody and a solution containing an enzyme-labeled second antibody are fed successively to a microporous membrane on which an antigen is immobilized, to form a complex of the immobilized antigen, the antibody and the enzyme-labeled second antibody. Thereafter, an enzyme substrate is fed to the membrane to cause a luminous reaction on the membrane. The existence and the quantity of the antibody are detected based on the luminous quantity. The use of chemiluminescence enables to detect the antibody at extremely high sensitivity. When the luminescence is detected by means of a photography film, adjustment of the exposure time enables to set up the sensitivity at any magnitude. Especially, use of an instant photography film can provide an extremely convenient and rapid detection of the antibody.

  将含有分析抗体的液体样品和含有酶标记第二抗体的溶液依次送入固定了抗原的微孔膜，以形成固定抗原、抗体和酶标记第二抗体的复合物。然后，将酶底物送入膜上，使膜上发生发光反应。根据发光量检测抗体的存在和数量。使用化学发光技术能以极高的灵敏度检测抗体。当使用照相胶片检测发光时，通过调整曝光时间，可以设定任何灵敏度。特别是使用即时照相胶片可以极其方便快捷地检测抗体。
• METHOD FOR ASSAY ON THE EFFECT OF VASCULARIZATION INHIBITOR
  申请人：Eisai R&D Management Co., Ltd.

  公开号：EP1925676A1

  公开（公告）日：2008-05-28

  The present invention provides a method of predicting the antitumor effect of an angiogenesis inhibitor. It is possible to predict the antitumor effect of an angiogenesis inhibitor by evaluating the EGF dependency of a tumor cell for proliferation and/or survival and using the EGF dependency as an indicator. Since the antitumor effect of an angiogenesis inhibitor correlates with the EGF dependency of a tumor cell for proliferation and/or survival, the angiogenesis inhibitors is capable of producing excellent antitumor effect when combined with a substance having EGF inhibitory activity.

  本发明提供了一种预测血管生成抑制剂抗肿瘤效果的方法。通过评估肿瘤细胞增殖和/或存活对 EGF 的依赖性，并将 EGF 依赖性作为指标，可以预测血管生成抑制剂的抗肿瘤效果。由于血管生成抑制剂的抗肿瘤效果与肿瘤细胞增殖和/或存活对 EGF 的依赖性相关，因此血管生成抑制剂与具有 EGF 抑制活性的物质结合使用时，能够产生极佳的抗肿瘤效果。
• Methods for diagnosing irritable bowel syndrome
  申请人：Nestec S.A.

  公开号：EP2778685A1

  公开（公告）日：2014-09-17

  The invention provides an ELISA assay for the determination of serum mast cell β-tryptase levels using rabbit anti-tryptase as the capture antibody and alkaline phosphatase conjugated G3 as the detecting antibody. Luminescent substrate CPSD was used to enhance the assay sensitivity. Also provided are methods for aiding in the diagnosis of irritable bowel syndrome by detecting the serum level of β-tryptase, histamine and/or prostaglandin E2.

  本发明提供了一种测定血清肥大细胞β-色氨酸酶水平的酶联免疫吸附试验，使用兔抗色氨酸酶作为捕获抗体，碱性磷酸酶共轭 G3 作为检测抗体。发光底物 CPSD 用于提高检测灵敏度。还提供了通过检测血清中 β-色氨酸酶、组胺和/或前列腺素 E2 的水平来帮助诊断肠易激综合征的方法。
• MiRNAs enhancing cell productivity
  申请人：Hochschule Biberach

  公开号：EP2937417A1

  公开（公告）日：2015-10-28

  The invention relates to a nucleic acid construct comprising at least two different regions, wherein the regions are selected of a first region encoding for at least one miRNA stimulating cellular production of a biomolecule in a cell, a second region encoding for at least one miRNA and/or miRNA-inhibitor suppressing cell death, and a third region encoding for at least one miRNA and/or miRNA-inhibitor regulating cell proliferation. The invention further relates to a cell comprising a respective nucleic acid construct and to a method for increasing the yield of a biomolecule produced by a cell cultured in vitro comprising at least two steps selected of stimulating cellular production of the biomolecule, reducing cell death, and regulating proliferation.

  本发明涉及一种包含至少两个不同区域的核酸构建体，其中所述区域选自编码至少一种刺激细胞产生生物大分子的miRNA的第一区域、编码至少一种抑制细胞死亡的miRNA和/或miRNA抑制剂的第二区域以及编码至少一种调节细胞增殖的miRNA和/或miRNA抑制剂的第三区域。本发明还涉及一种包含各自核酸构建体的细胞，以及一种提高体外培养细胞产生的生物大分子产量的方法，该方法包括至少两个步骤，分别选自刺激细胞产生生物大分子、减少细胞死亡和调节增殖。