10--decanoic acid | 175234-79-4

• 分子结构分类: 有机化合物 - 苯类化合物 - 三苯基化合物
• 英文名称: 10--decanoic acid
• 英文别名: 10-[Bis(4-methoxyphenyl)-phenylmethoxy]decanoic acid
• CAS: 175234-79-4
• 化学式: C₃₁H₃₈O₅
• 分子量: 490.64
• InChiKey: LVKOVCDXGYJSHC-UHFFFAOYSA-N

物化性质

• 沸点：
  619.5±55.0 °C(predicted)
• 密度：
  1.097±0.06 g/cm3(Temp: 20 °C; Press: 760 Torr)(predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  7.4
• 重原子数：
  36
• 可旋转键数：
  16
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.39
• 拓扑面积：
  65
• 氢给体数：
  1
• 氢受体数：
  5

反应信息

• 作为反应物：
  描述：

  对硝基苯酚 、 10--decanoic acid 在 N,N'-二环己基碳二亚胺 作用下， 以 1,4-二氧六环 为溶剂， 生成 (4-Nitrophenyl) 10-[bis(4-methoxyphenyl)-phenylmethoxy]decanoate
  参考文献：
  名称：

  使用硅胶载体在线固相合成寡核苷酸-肽杂化物

  摘要：

  描述了通过自动合成制备寡核苷酸-肽杂合分子的方法。结合物已在包括CPG（可控孔玻璃）和Fractosil载体的二氧化硅载体上组装。新颖Ñ ε -赖氨酸的保护基，使用1-（4,4-二甲基-2,6-二氧代环己-1-亚基）乙基（DDE）。

  DOI：

  10.1016/0040-4039(94)85218-9
• 作为产物：
  描述：

  4,4'-双甲氧基三苯甲基氯 在 4-二甲氨基吡啶 、 lithium hydroxide 、 N,N-二异丙基乙胺 作用下， 以 四氢呋喃 、 甲醇 、 二氯甲烷 、 水 为溶剂， 生成 10--decanoic acid
  参考文献：
  名称：

  Libraries of Composite Polyfluors Built from Fluorescent Deoxyribosides

  摘要：

  We report on a new class of water-soluble fluorescent molecules (polyfluors) that are composed of multiple individual fluorophores assembled on a DNA-like backbone. Four fluorophore deoxyribosides were synthesized, and these individual molecules were assembled into oligofluor strings on a DNA synthesizer. A library of 256 tetrafluors was generated by split and pool methods on polystyrene beads. Images of the library under a fluorescence microscope revealed at least 40-50 different hues and intensities. Selected tetrafluors were resynthesized in pure form in solution and displayed properties, such as large Stokes shifts, that individual fluorophores do not have.

  DOI：

  10.1021/ja027197a

文献信息

• Fluorescent xDNA nucleotides as efficient substrates for a template-independent polymerase
  作者：Sarah K. Jarchow-Choy、Andrew T. Krueger、Haibo Liu、Jianmin Gao、Eric T. Kool

  DOI：10.1093/nar/gkq853

  日期：2011.3

  Template independent polymerases, and terminal deoxynucleotidyl transferase (TdT) in particular, have been widely used in enzymatic labeling of DNA 3′-ends, yielding fluorescently-labeled polymers. The majority of fluorescent nucleotides used as TdT substrates contain tethered fluorophores attached to a natural nucleotide, and can be hindered by undesired fluorescence characteristics such as self-quenching. We previously documented the inherent fluorescence of a set of four benzo-expanded deoxynucleoside analogs (xDNA) that maintain Watson–Crick base pairing and base stacking ability; however, their substrate abilities for standard template-dependent polymerases were hampered by their large size. However, it seemed possible that a template-independent enzyme, due to lowered geometric constraints, might be less restrictive of nucleobase size. Here, we report the synthesis and study of xDNA nucleoside triphosphates, and studies of their substrate abilities with TdT. We find that this polymerase can incorporate each of the four xDNA monomers with kinetic efficiencies that are nearly the same as those of natural nucleotides, as measured by steady-state methods. As many as 30 consecutive monomers could be incorporated. Fluorescence changes over time could be observed in solution during the enzymatic incorporation of expanded adenine (dxATP) and cytosine (dxCTP) analogs, and after incorporation, when attached to a glass solid support. For (dxA)n polymers, monomer emission quenching and long-wavelength excimer emission was observed. For (dxC)n, fluorescence enhancement was observed in the polymer. TdT-mediated synthesis may be a useful approach for creating xDNA labels or tags on DNA, making use of the fluorescence and strong hybridization properties of the xDNA.

  与模板无关的聚合酶，特别是末端脱氧核苷酸转移酶（TdT），已被广泛用于对 DNA 3′端进行酶标记，从而获得荧光标记的聚合物。用作 TdT 底物的大多数荧光核苷酸都含有连接到天然核苷酸上的系留荧光团，并且可能会受到自淬等不期望的荧光特性的阻碍。我们以前记录了一组四种苯扩增脱氧核苷类似物（xDNA）的固有荧光，这些类似物保持了沃森-克里克碱基配对和碱基堆叠能力；然而，它们对于标准模板依赖性聚合酶的底物能力却因体积过大而受到阻碍。然而，不依赖模板的酶由于降低了几何限制，对核碱基大小的限制似乎可能较小。在这里，我们报告了 xDNA 核苷三磷酸酯的合成和研究，以及它们与 TdT 底物能力的研究。我们发现，根据稳态方法测量，这种聚合酶能以与天然核苷酸几乎相同的动力学效率结合四种 xDNA 单体中的每一种。可结合多达 30 个连续单体。在酶法结合扩增的腺嘌呤（dxATP）和胞嘧啶（dxCTP）类似物的过程中，可以观察到溶液中的荧光随时间的变化，而在结合后，当连接到玻璃固体支持物上时，也可以观察到荧光随时间的变化。对于 (dxA)n 聚合物，观察到单体发射淬灭和长波长准分子发射。对于 (dxC)n，在聚合物中观察到荧光增强。利用 xDNA 的荧光和强杂交特性，TdT 介导的合成可能是在 DNA 上创建 xDNA 标签或标记的有用方法。
• MODIFIED DOUBLE-STRANDED POLYNUCLEOTIDE
  申请人：Koizumi Makoto

  公开号：US20120220649A1

  公开（公告）日：2012-08-30

  The present invention provides a double-stranded polynucleotide having a sense strand polynucleotide consisting of a nucleotide sequence complementary to a target sequence in a target gene, and an antisense strand polynucleotide having a nucleotide sequence complementary to the sense strand polynucleotide, wherein an aryl or heteroaryl compound is bound to a phosphate group at the 5′-end of the antisense strand polynucleotide.

  本发明提供了一种双链聚核苷酸，其具有一个具有与目标基因中的目标序列互补的核苷酸序列的正链聚核苷酸，以及一个具有与正链聚核苷酸互补的核苷酸序列的反义链聚核苷酸，其中一种芳基或杂环芳基化合物结合到反义链聚核苷酸的5'-末端的磷酸基团上。
• Modified Single-Stranded Polynucleotide
  申请人：Koizumi Makoto

  公开号：US20130253038A1

  公开（公告）日：2013-09-26

  It is intended to provide a polynucleotide that is resistant to RNase and has an RNA interference effect, etc. The present invention provides a single-stranded polynucleotide that is derived from a double-stranded polynucleotide comprising a sense strand polynucleotide corresponding to a target gene, and an antisense strand polynucleotide having a nucleotide sequence complementary to the sense strand polynucleotide, and has a structure in which the 5′-end of the antisense strand and the 3′-end of the sense strand are linked via a phenyl group-containing linker to form a phosphodiester structure at each of these ends.

  本发明旨在提供一种对RNase具有抗性并具有RNA干扰效应的多核苷酸。本发明提供了一种单链多核苷酸，其源自于包含与目标基因相对应的正义链多核苷酸和具有与正义链多核苷酸互补的核苷酸序列的反义链多核苷酸的双链多核苷酸，且具有一种结构，其中反义链的5'-末端和正义链的3'-末端通过含苯基的连接剂连接形成磷酸二酯结构。
• MODIFIED SINGLE-STRAND POLYNUCLEOTIDE
  申请人：Daiichi Sankyo Company, Limited

  公开号：EP2647713A1

  公开（公告）日：2013-10-09

  It is intended to provide a polynucleotide that is resistant to RNase and has an RNA interference effect, etc. The present invention provides a single-stranded polynucleotide that is derived from a double-stranded polynucleotide comprising a sense strand polynucleotide corresponding to a target gene, and an antisense strand polynucleotide having a nucleotide sequence complementary to the sense strand polynucleotide, and has a structure in which the 5'-end of the antisense strand and the 3'-end of the sense strand are linked via a phenyl group-containing linker to form a phosphodiester structure at each of these ends.

  本发明旨在提供一种能抗 RNase 并具有 RNA 干扰作用的多核苷酸等。本发明提供了一种由双链多核苷酸衍生的单链多核苷酸，双链多核苷酸包括与靶基因相对应的有义链多核苷酸和具有与有义链多核苷酸互补的核苷酸序列的反义链多核苷酸、其结构是反义链的 5'-端和有义链的 3'-端通过含苯基的连接体连接，在这两端分别形成磷酸二酯结构。
• Synthesis, purification and characterization of two peptide-oligonucleotide conjugates as potential artificial nucleases
  作者：Jean-Christophe Truffert、Ulysse Asseline、André Brack、Nguyen T. Thuong

  DOI：10.1016/0040-4020(96)00018-x

  日期：1996.2

  Two peptide-oligonucleotide conjugates 10a and 10b, containing as their peptide moiety the active site mimic of ribonuclease A (HGH motif) and the Cu (ID complexing metallopeptide (GGH motif) respectively, have been synthesized by original on-line solid phase synthesis using pentafluorophenyl active esters and Boc-His(Tos)-OH. Mild basic conditions for the final deprotection and reversed-phase purification afforded the pure hybrid molecules in good yields. The conjugates have been characterized by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.