| 1246861-09-5

• 分子结构分类: 有机化合物 - 苯类化合物 - 三苯基化合物
• CAS: 1246861-09-5
• 化学式: C₄₂H₄₄N₄O₇
• 分子量: 716.834
• InChiKey: HGGWFWGZPIOSGV-LUUYORSZSA-N

物化性质

• 沸点：
  803.9±75.0 °C(predicted)
• 密度：
  1.32±0.1 g/cm3(Temp: 20 °C; Press: 760 Torr)(predicted)

反应信息

• 作为反应物：
  描述：

  、 双(二异丙基氨基)(2-氰基乙氧基)膦 在 二异丙基铵盐四氮唑 作用下， 以 二氯甲烷 为溶剂， 反应 48.0h， 以111 mg的产率得到
  参考文献：
  名称：

  Large flanking sequence effects in single nucleotide mismatch detection using fluorescent nucleoside Çf

  摘要：

  The first systematic study of flanking sequence effects on mismatch detection by a fluorescent nucleotide is described, using fluoroside C-f. Although a high degree of variance was observed in fluorescence intensity of mismatched duplexes between different flanking sequences, C-f was able to distinguish a mismatch from the fully base-paired duplex in 13 out of 16 sequences, and even identify each mismatch in 10 of those flanking sequences. For the flanking sequences where fluoroside C-f did not unambiguously determine its base-pairing partner, the experimental conditions were varied in an attempt to facilitate mismatch identification. No beneficial effect on the relative fluorescence intensities was achieved by changing the temperature, adding organic co-solvents or potassium iodide. In contrast, mercuric ions selectively quenched the fluorescence intensity of the C-f.T mismatch, effectively resolving the overlap of all emission spectra and thereby facilitating identification of all base-pairing partners in any flanking sequence by C-f. This is the first time mercuric ions have been used to selectively quench the fluorescence of a single mismatch. A noticeable characteristic of C-f is that, unlike most fluorosides, the fluorescence intensity of C-f was not quenched to a discernable degree by a flanking G-C pair. (C) 2010 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmc.2010.06.060
• 作为产物：
  描述：

  6-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-13,13,15,15-tetramethyl-9-oxa-2,4,6,14-tetrazatetracyclo[8.7.0.03,8.012,16]heptadeca-1(10),3,7,11,16-pentaen-5-one 、 4,4'-双甲氧基三苯甲基氯 在 4-二甲氨基吡啶 、 吡啶 作用下， 反应 13.17h， 生成
  参考文献：
  名称：

  Large flanking sequence effects in single nucleotide mismatch detection using fluorescent nucleoside Çf

  摘要：

  The first systematic study of flanking sequence effects on mismatch detection by a fluorescent nucleotide is described, using fluoroside C-f. Although a high degree of variance was observed in fluorescence intensity of mismatched duplexes between different flanking sequences, C-f was able to distinguish a mismatch from the fully base-paired duplex in 13 out of 16 sequences, and even identify each mismatch in 10 of those flanking sequences. For the flanking sequences where fluoroside C-f did not unambiguously determine its base-pairing partner, the experimental conditions were varied in an attempt to facilitate mismatch identification. No beneficial effect on the relative fluorescence intensities was achieved by changing the temperature, adding organic co-solvents or potassium iodide. In contrast, mercuric ions selectively quenched the fluorescence intensity of the C-f.T mismatch, effectively resolving the overlap of all emission spectra and thereby facilitating identification of all base-pairing partners in any flanking sequence by C-f. This is the first time mercuric ions have been used to selectively quench the fluorescence of a single mismatch. A noticeable characteristic of C-f is that, unlike most fluorosides, the fluorescence intensity of C-f was not quenched to a discernable degree by a flanking G-C pair. (C) 2010 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmc.2010.06.060