2,5-dimethoxy-4-[(4-nitrophenyl)diazenyl]aniline | 6358-51-6

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 偶氮苯
• 英文名称: 2,5-dimethoxy-4-[(4-nitrophenyl)diazenyl]aniline
• 英文别名: (E)-2,5-dimethoxy-4-((4-nitrophenyl)diazenyl)aniline；2,5-dimethoxy-4-(4-nitro-phenylazo)-aniline；2,5-Dimethoxy-4-(4-nitro-phenylazo)-anilin；4'-Nitro-4-amino-2.5-dimethoxy-azobenzol；p-nitroanilin->2,5-dimethoxyanilin
• CAS: 6358-51-6
• 化学式: C₁₄H₁₄N₄O₄
• 分子量: 302.29
• InChiKey: KBJGBAOGDZOYIZ-WUKNDPDISA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.61
• 重原子数：
  22.0
• 可旋转键数：
  5.0
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.14
• 拓扑面积：
  112.34
• 氢给体数：
  1.0
• 氢受体数：
  7.0

安全信息

• 海关编码：
  2927000090

反应信息

• 作为反应物：
  描述：

  3-(methyl(phenyl)amino)propane-1,2-diol 、 2,5-dimethoxy-4-[(4-nitrophenyl)diazenyl]aniline 在 盐酸 、 sodium nitrite 、 sodium acetate 作用下， 以 甲醇 、 水 为溶剂， 以70%的产率得到3-((4-((E)-(2,5-dimethoxy-4-((E)-(4-nitrophenyl)diazenyl)phenyl)diazenyl)phenyl)(methyl)amino)propane-1,2-diol
  参考文献：
  名称：

  Diol荧光素硼酸猝灭剂

  摘要：

  用3-氨基苯基硼酸处理异硫氰酸荧光素，提供带有荧光标记的硼酸衍生物，该衍生物可用于评估硼酸酯形成后用一系列定制的二醇附加猝灭剂进行的Förster共振能量转移（FRET）猝灭。荧光光谱法比较荧光素及其硼酸附加同源物与附加淬灭剂的二醇处理之间的淬灭效率，发现硼酸酯形成（共价连接）是更有效的方案，并且从淬灭剂组中也包括核苷。

  DOI：

  10.1002/asia.200900386
• 作为产物：
  描述：

  acetic acid-[2,5-dimethoxy-4-(4-nitro-phenylazo)-anilide] 生成 2,5-dimethoxy-4-[(4-nitrophenyl)diazenyl]aniline
  参考文献：
  名称：

  DE744215

  摘要：

  公开号：

文献信息

• 3-ARYL PROPIOLONITRILE COMPOUNDS FOR THIOL LABELING
  申请人：UNIVERSITE DE STRASBOURG

  公开号：US20160145199A1

  公开（公告）日：2016-05-26

  The present invention relates to a process for labeling compounds comprising thiol moieties with 3-arylpropiolonitrile compounds, to 3-arylpropiolonitrile compounds substituted with tag moieties and to specific 3-arylpropiolonitrile linkers.

  本发明涉及一种将含硫醇基团的化合物用3-芳基丙炔腈化合物标记的方法，以及用标记基团取代的3-芳基丙炔腈化合物和特定的3-芳基丙炔腈连接剂。
• Non-fluorescent quencher compounds and biomolecular assays
  申请人：——

  公开号：US20030082547A1

  公开（公告）日：2003-05-01

  Bis-diazo,triaryl and aryldiazo-N-arylphenazonium quencher moieties, substituted with electron-withdrawing and electron-donating substituents which induce polarity in the delocalized aryl/diazo ring systems, are useful as labels when attached to biomolecules such as polynucleotides, nucleosides, nucleotides, and polypeptides. The quencher moieties are non-fluorescent and accept energy from fluorescent reporter labels by any energy-transfer mechanism, such as FRET. Fluorescence quencher compositions are useful in preparing quencher labelled biomolecules for various molecular biology assays based on fluorescence detection. 1

  双重重氮基、三芳基和芳基重氮-N-芳基苯酚盐猝灭基团，通过带电子吸引和电子给予取代基团引起芳基/重氮环系统极性，当连接到生物分子如多核苷酸、核苷、核苷酸和多肽时，可作为标签。这些猝灭基团是非荧光的，并通过任何能量转移机制（如FRET）从荧光报告标签吸收能量。荧光猝灭剂组合物在制备带有猝灭剂标记的生物分子，用于基于荧光检测的各种分子生物学分析中，非常有用。
• Preparation of FRET reporters to support chemical probe development
  作者：Timothy L. Foley、Adam Yasgar、Christopher J. Garcia、Ajit Jadhav、Anton Simeonov、Michael D. Burkart

  DOI：10.1039/c0ob00322k

  日期：——

  In high throughput screening (HTS) campaigns, the quality and cost of commercial reagents suitable for pilot studies often create obstacles upon scale-up to a full screen. We faced such challenges in our efforts to implement an HTS for inhibitors of the phosphopantetheinyl transferase Sfp using an assay that had been validated using commercially available reagents. Here we demonstrate a facile route to the synthetic preparation of reactive tetraethylrhodamine and quencher probes, and their application to economically produce fluorescent and quencher-modified substrates. These probes were prepared on a scale that would allow a full, quantitative HTS of more than 350,000 compounds.

  在高通量筛选（HTS）活动中，适用于初步研究的商业试剂的质量和成本往往在扩大到全面筛选时形成障碍。我们在实施针对磷酸泛妥西转移酶Sfp抑制剂的HTS时，就面临着这样的挑战，该筛选所用的检测方法已通过商业可用试剂的验证。在此，我们展示了一种简便的合成方法，用于制备反应性四乙基罗丹明和淬灭剂探针，并将其应用于经济地生产荧光标记和淬灭剂修饰的底物。这些探针的制备规模足以支持对超过350,000个化合物的全面定量HTS。
• Surface Functionalisation Using Arylcarbene Reactive Intermediates
  申请人：Moloney Mark

  公开号：US20080146731A1

  公开（公告）日：2008-06-19

  A process for producing a substrate having a functionalised surface, which process comprises contacting the substrate with a carbene precursor, which carbene precursor is a compound of formula (III) or (IV) whose substituent groups are defined herein: (b) generating a carbene reactive intermediate from the carbene precursor so that it reacts with the substrate to functionalise the surface, thereby yielding an activated substrate; and (c) further functionalising the activated substrate obtained in (b). In (c), the activated substrate may be further functionalised by treating the activated substrate with a diazonium salt for the introduction of colour and/or another desired activity, and/or by treatment with hydrogen peroxide to produce a biocidal substrate. The invention further relates to carbene precursor compounds for use in the surface functionalisation process, and to processes for preparing certain precursor compounds.

  一种生产具有功能化表面的基板的方法，该方法包括将基板与卡宾前体接触，所述卡宾前体是式（III）或（IV）的化合物，其取代基团在此定义：（b）从卡宾前体生成卡宾反应中间体，使其与基板反应以功能化表面，从而产生活化基板；（c）进一步功能化在（b）中获得的活化基板。在（c）中，可以通过用重氮盐处理活化基板来进一步功能化活化基板，以引入颜色和/或其他所需活性，并/或通过过氧化氢处理来产生生物杀灭基板。本发明还涉及用于表面功能化过程的卡宾前体化合物，以及制备某些前体化合物的过程。
• Novel substituted-polyaryl chromophoric compounds
  申请人：——

  公开号：US20040013375A1

  公开（公告）日：2004-01-22

  The invention provides for novel substituted-polyaryl chromophoric compounds which desirably comprise a single diazo group, and optimally include a plurality of diazo groups. Preferably the chromophores exhibit optical nonlinear second-order properties and have unique absorption maximum and other chromophoric properties that make them useful for, among other things, muitifonctional optical switches or waveguides.

  本发明提供了一种新型的取代聚芳基色团化合物，其理想地包含一个单一的重氮基团，并且最好包括多个重氮基团。优选的是，这些色团表现出光学非线性二阶性质，并具有独特的吸收峰和其他色团性质，使它们在多功能光学开关或波导等方面非常有用。