3-[Amino-bis(2-methylphenyl)methyl]-4-methylbenzene-1,2-diamine;hydrochloride

• 分子结构分类: 有机化合物 - 苯类化合物 - 三苯基化合物
• 英文名称: 3-[Amino-bis(2-methylphenyl)methyl]-4-methylbenzene-1,2-diamine;hydrochloride
• 英文别名: 3-[amino-bis(2-methylphenyl)methyl]-4-methylbenzene-1,2-diamine;hydrochloride
• 化学式: C22H26ClN3
• 分子量: 367.9
• InChiKey: CRXGQNSHYNLPRJ-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  4.45
• 重原子数：
  26
• 可旋转键数：
  3
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.18
• 拓扑面积：
  78.1
• 氢给体数：
  4
• 氢受体数：
  3

文献信息

• RT-qPCR analysis of micro-dissected material from stained FFPET section
  申请人：Roche Molecular Systems, Inc.

  公开号：US10059980B2

  公开（公告）日：2018-08-28

  The present invention refers to a method for immuno-histochemical staining of a formalin-fixed, paraffin-embedded tissue section comprising the steps of a) providing a solid support, b) mounting the formalin-fixed, paraffin-embedded tissue section onto the solid support, c) removing the paraffin from the formalin-fixed, paraffin-embedded tissue section, d) heating the tissue section mounted on the solid support to retrieve epitopes at 50 to 70° C. for 12 to 24 h, and e) staining the tissue section mounted on the solid support, wherein at least step e) is performed in the presence of 0.5 to 3.0 M sodium chloride. The present invention further refers to a kit for performing the method.

  本发明涉及一种对福尔马林固定、石蜡包埋的组织切片进行免疫组织化学染色的方法，包括以下步骤 a) 提供固体支持物；b) 将福尔马林固定、石蜡包埋的组织切片安装到固体支持物上；c) 从福尔马林固定、石蜡包埋的组织切片上去除石蜡；d) 在 50 至 70° C 下加热安装在固体支持物上的组织切片以获取表位，时间为 12 至 24 小时；以及 e) 对安装在固体支持物上的组织切片进行染色。其中至少步骤 e) 是在 0.5 至 3.0 M 氯化钠存在下进行的。本发明还涉及一种用于实施该方法的试剂盒。
• RT-QPCR ANALYSIS OF MICRO-DISSECTED MATERIAL FROM STAINED FFPET SECTION
  申请人：Roche Diagnostics GmbH

  公开号：EP2948750A1

  公开（公告）日：2015-12-02
• RT-qPCR Analysis of Micro-Dissected Material from Stained FFPET Section
  申请人：Roche Molecular Systems, Inc.

  公开号：US20180346964A1

  公开（公告）日：2018-12-06

  The present invention refers to a method for immuno-histochemical staining of a formalin-fixed, paraffin-embedded tissue section comprising the steps of a) providing a solid support, b) mounting the formalin-fixed, paraffin-embedded tissue section onto the solid support, c) removing the paraffin from the formalin-fixed, paraffin-embedded tissue section, d) heating the tissue section mounted on the solid support to retrieve epitopes at 50 to 70° C. for 12 to 24 h, and e) staining the tissue section mounted on the solid support, wherein at least step e) is performed in the presence of 0.5 to 3.0 M sodium chloride. The present invention further refers to a kit for performing the method.
• [EN] RT-QPCR ANALYSIS OF MICRO-DISSECTED MATERIAL FROM STAINED FFPET SECTION<br/>[FR] ANALYSE PAR RT-QPCR DE MATÉRIAU MICRO-DISSÉQUÉ D'UNE COUPE DE TFFIP COLORÉE
  申请人：ROCHE DIAGNOSTICS GMBH

  公开号：WO2014114650A1

  公开（公告）日：2014-07-31

  The present invention refers to a method for immuno-histochemical staining of a formalin-fixed, paraffin-embedded tissue section comprising the steps of a) providing a solid support, b) mounting the formalin-fixed, paraffin-embedded tissue section onto the solid support, c) removing the paraffin from the formalin-fixed, paraffin-embedded tissue section, d) heating the tissue section mounted on the solid support to retrieve epitopes at 50 to 70°C for 12 to 24 h, and e) staining the tissue section mounted on the solid support, wherein at least step e) is performed in the presence of 0.5 to 3.0 M sodium chloride. The present invention further refers to a kit for performing the method.