sinapate 1-glucosyltransferase | 74082-53-4

• 分子结构分类: 转移酶 - 糖基转移酶 - 己糖基转移酶
• 英文名称: sinapate 1-glucosyltransferase
• 英文别名: uridine diphosphoglucose-sinapate glucosyltransferase；UDP-glucose:sinapic acid glucosyltransferase；uridine 5'-diphosphoglucose-hydroxycinnamic acid acylglucosyltransferase；UDP-glucose:sinapate D-glucosyltransferase；UDP-glucose:sinapic acid glucosyltransferase (EC 2.4.1.120)
• CAS: 74082-53-4

反应信息

• 作为试剂：
  描述：

  尿苷(5')二氢二磷酰(1)-alpha-D-葡萄糖 、 芥子酸 在 sinapate 1-glucosyltransferase 2-吗啉乙磺酸 、 甘油 、 2-巯基乙醇 作用下， 以 甲醇 、 水 为溶剂， 反应 0.25h， 生成 1‐O‐sinapoyl‐beta‐D‐glucose
  参考文献：
  名称：

  Enzymology of udp-glucose:sinapic acid glucosyltransferase from brassica napus

  摘要：

  UDP-glucose:sinapic acid glucosyltransferase (SGT; EC 2.4.1.120) was purified from 60-h-old seedlings of Brassica napus. The purified SGT appears to be a cytosolic monomeric polypeptide with a M-r of 42 kDa and a pI of 5. Kinetic analysis suggested that the catalytic mechanism used by SGT best fits a "random bi-bi" model, with a K m(UDP-glucose) of 2.4 mM and K-m(sinapic acid) of 0.16 mM. SGT also catalyzes the reverse reaction in vitro, using UDP and sinapoylglucose to form UDP-glucose. No cofactors are required for enzyme activity, but reducing agents and glycerol are required to stabilize the activity. The enzyme is strongly inhibited by p-OH-mercuribenzoic acid, UDP, TDP, Zn++, Cu++ and Hg++. (C) 1998 Elsevier Science Ltd. All rights reserved.

  DOI：

  10.1016/s0031-9422(98)00252-0