UDP-galactopyranose mutase | 174632-18-9

• 分子结构分类: 异构酶 - 分子内转移酶 - 转移基团
• 英文名称: UDP-galactopyranose mutase
• 英文别名: UGM；UDP-D-galactopyranose furanomutase
• CAS: 174632-18-9

反应信息

• 作为反应物：
  描述：

  UDP-galactopyranose mutase 生成 UDP-galactopyranose mutase reduced form
  参考文献：
  名称：

  摘要：

  DOI：
• 作为试剂：
  描述：

  uridine 5'-(2-deoxy-2-fluoro-α-D-galactopyranosyl diphosphate) 在 UDP-galactopyranose mutase 、 腺嘌呤黄素 、 1,4-二巯基-2,3-丁二醇 作用下， 生成
  参考文献：
  名称：

  Enzymatic synthesis of UDP-(3-deoxy-3-fluoro)-d-galactose and UDP-(2-deoxy-2-fluoro)-d-galactose and substrate activity with UDP-galactopyranose mutase

  摘要：

  The novel UDP-sugar uridine 5'-(3-deoxy-3-fluoro-D-galactopyranosyl diphosphate) (I) and UDP-(2-deoxy-2-fluoro)-D-galactose (2) have been prepared enzymatically and tested as substrate analogues for the enzyme UDP-galactopyranose mutase (UDP-Galp mutase EC 5.4.99.9). Turnover of both 1 and 2 by UDP-Galp mutase was observed by HPLC and F-19 NMR. The HPLC elution profile and F-19 chemical shift of the products are consistent with the formation of the predicted furanose forms of 1 and 2. The K-m values for compounds 1 and 2 were similar to those of the natural substrate UDP-Galp (0.26 mM for 1, 0.2 mM for 2, and 0.6 mM for UDP-Galp), but the values for k(cat) were substantially different (1.6/min for I, 0.02/min for 2, and 1364/min for UDP-Galp). A correlation was also observed between the equilibrium yield of product formed during turnover of UDP-sugar by UDP-Galp mutase (UDP-Galp, compound 1 or compound 2), and the amount of furanose present for the free sugar at thermal equilibrium in aqueous solution, using H-1 and F-19 NMR spectroscopy. The implications of these results to the mechanism of the unusual enzymatic reaction are discussed. (C) 2000 Elsevier Science Ltd. All rights reserved.

  DOI：

  10.1016/s0008-6215(00)00135-x