5-(3-(amino)prop-1-ynyl)-3',5'-O-bisdimethoxytrityl-2'-deoxyuridine | 169515-44-0

• 分子结构分类: 有机化合物 - 苯类化合物 - 三苯基化合物
• 英文名称: 5-(3-(amino)prop-1-ynyl)-3',5'-O-bisdimethoxytrityl-2'-deoxyuridine
• 英文别名: 5-(3-aminoprop-1-ynyl)-1-[(2R,4S,5R)-4-[bis(4-methoxyphenyl)-phenylmethoxy]-5-[[bis(4-methoxyphenyl)-phenylmethoxy]methyl]oxolan-2-yl]pyrimidine-2,4-dione
• CAS: 169515-44-0
• 化学式: C₅₄H₅₁N₃O₉
• 分子量: 886.014
• InChiKey: YOOFOTRLRWUONT-SEWIUPLBSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  7.7
• 重原子数：
  66
• 可旋转键数：
  17
• 环数：
  8.0
• sp3杂化的碳原子比例：
  0.22
• 拓扑面积：
  140
• 氢给体数：
  2
• 氢受体数：
  10

文献信息

• Labelling and detection of nucleic acids
  申请人：Cruachem Limited

  公开号：EP0754700A2

  公开（公告）日：1997-01-22

  The invention described relates to 2,4-dinitrophenyl (DNP) labelling of nucleic acids or nucleic acid probes. Specifically, there is described compounds of formula I. A compound of formula I:         X-Q-R*-NH-DNP     (I) wherein DNP is a 2,4-dinitrophenyl group; R* is a C1-12 carbon chain, optionally interrupted with one or more of the following: -0-, -NH-, alkylamine, carbon-carbon double bond, carbon-carbon triple bond, carbonyl group or amide group; Q is a group Q* or a group wherein t is O or is an integer of from 1 to 6; Q* is a nucleoside comprising a heterocyclic amine base bonded to C1' of a C5 or C6 pentose sugar; X is a group and where group Q represents a group Q*, X may also be a group or a phosphate, diphosphate or triphosphate group; R3 and R4 are each independently selected from C1-6 alkyl, cycloalkyl, aralkyl and aryl groups; and R5 and R6 are each blocking groups which may be the same or different and the use of such compounds in the synthesis of oligonucleotide probes and formation of DNP-labelled oligonucleotides for use in solid-phase PCR, solid phase in situ hybridisation or in affinity chromatography.

  所述发明涉及2,4-二硝基苯基（DNP）标记核酸或核酸探针。具体而言，描述了式I的化合物。式I的化合物：X-Q-R *-NH-DNP（I），其中DNP是2,4-二硝基苯基；R *是C1-12碳链，可选地中断一个或多个以下的部分：-0-，-NH-，烷基胺，碳-碳双键，碳-碳三键，羰基或酰胺基；Q是Q *基团或者一个基团，其中t是O或1到6的整数；Q *是一种核苷酸，包括一种杂环胺基与C5或C6脱氧核糖的C1'键合；X是一个基团，其中基团Q代表一个Q *基团时，X也可以是一个基团或磷酸、二磷酸或三磷酸基团；R3和R4各自独立地选自C1-6烷基，环烷基，芳基烷基和芳基基团；R5和R6是可以相同或不同的阻断基团，以及在合成寡核苷酸探针和形成DNP标记的寡核苷酸中的使用，用于固相PCR，固相原位杂交或亲和层析。