The present invention provides a method for analyzing leukocytes, by which the leukocytes can be classified and measured stably with high accuracy even when a dilution ratio of a sample containing the leukocytes is low or a flow velocity during the analysis is slow, and an analysis reagent used for the analysis method. The analysis method of the present invention includes the steps of mixing a sample containing leukocytes and erythrocytes and an analysis reagent containing a surfactant that reacts with leukocytes; and measuring the leukocytes by passing a mixed solution of the sample and the analysis reagent through a fine through-hole, measuring a signal detected when the mixed solution passes through the fine through-hole, and classifying and counting the leukocytes in the sample. The analysis reagent further contains a nonionic surfactant, and the nonionic surfactant has a sugar residue as a hydrophilic region and an aliphatic chain as a hydrophobic region.
本发明提供了一种分析白细胞的方法,通过该方法,即使含有白细胞的样品的稀释率较低或分析过程中流速较慢,也能稳定地对白细胞进行分类和测量,且准确度高;本发明还提供了一种用于该分析方法的
分析试剂。本发明的分析方法包括以下步骤:将含有白细胞和红细胞的样品与含有能与白细胞反应的
表面活性剂的
分析试剂混合;通过将样品和
分析试剂的混合溶液通过细通孔,测量混合溶液通过细通孔时检测到的信号,并对样品中的白细胞进行分类和计数,从而测量白细胞。
分析试剂还含有
非离子表面活性剂,
非离子表面活性剂的亲
水区域为糖残基,疏
水区域为
脂肪族链。