Enzymatic Total Synthesis of Defucogilvocarcin M and Its Implications for Gilvocarcin Biosynthesis
作者:Pallab Pahari、Madan K. Kharel、Micah D. Shepherd、Steven G. van Lanen、Jürgen Rohr
DOI:10.1002/anie.201105882
日期:2012.1.27
Teamwork: DefucogilvocarcinM (1, see scheme) was synthesized in a one‐pot, enzymatic reaction from acetyl‐CoA and malonyl‐CoA by a combination of 15 enzymes obtained from E. coli as well as the gilvocarcin, jadomycin, and ravidomycin biosynthetic pathways. The mixture of enzymes was systematically reduced and varied to further delineate the sequence of reactions in the complex, post‐polyketide steps
A one-pot enzymatic totalsynthesis of angucycline antibiotic rabelomycin was accomplished, starting from acetyl-CoA and malonyl-CoA, using a mixture of polyketide synthase (PKS) enzymes of the gilvocarcin, ravidomycin, and jadomycin biosynthetic pathways. The in vitro results were compared to in vivo catalysis using analogous sets of enzymes.