申请人:The Board of Trustees of the Leland Stanford Junior University
公开号:US10264974B2
公开(公告)日:2019-04-23
Disclosed are methods for imaging lumen-forming structures such as blood vessels using near-infrared fluorescence in the NIR-II region of 1000-1700 nm. The fluorescence is created by excitation of solubilized nano-structures that are delivered to the structures, such as carbon nanotubes, quantum dots or organic molecular fluorophores attached to hydrophilic polymers. These nanostructures fluoresce in the NIR-II region when illuminated through the skin and tissues. Fine anatomical vessel resolution down to −30 μm and high temporal resolution up to 5-10 frames per second is obtained for small-vessel imaging with up to 1 cm penetration depth in mouse hind limb, which compares favorably to tomographic imaging modalities such as CT and MRI with much higher spatial and temporal resolution, and compares favorably to scanning microscopic imaging techniques with much deeper penetration.
所公开的是利用 1000-1700 纳米 NIR-II 区域的近红外荧光对血管等管腔形成结构进行成像的方法。荧光是通过激发溶解的纳米结构产生的,这些纳米结构被输送到结构中,如碳纳米管、量子点或附着在亲水性聚合物上的有机分子荧光团。通过皮肤和组织照射时,这些纳米结构会在近红外-II 区发出荧光。在小鼠后肢穿透深度达 1 厘米的小血管成像中,可获得低至 -30 μm 的精细解剖血管分辨率和高达每秒 5-10 帧的高时间分辨率,与空间和时间分辨率更高的 CT 和 MRI 等断层成像模式相比毫不逊色,与穿透深度更深的扫描显微成像技术相比也毫不逊色。