荧光成像
荧光成像是利用荧光物质在特定波长的激发光照射下发出荧光进行成像的技术。该技术广泛应用于生物医药、有机化学和化学化工领域中。
基本原理荧光成像基于荧光物质（也称为荧光探针或荧光标记物）与特定分子之间的相互作用。荧光探针通常在受到外部能量激发时（如激光照射），会从基态跃迁至高能级状态，随后通过非辐射弛豫回到较低的激发态，并在此过程中释放出与其吸收波长相对应的荧光。
应用领域- 生物医学研究：利用标记有荧光探针的抗体、核酸或蛋白质对细胞内的特定目标进行定位和定量分析。
- 药物研发与筛选：通过在分子水平上观察化合物的作用效果，加速新药开发进程。
- 细胞生物学及分子生物学：如实时监测基因表达、细胞分裂等过程中的生物分子动态变化。
- 荧光探针的设计与合成：包括选择合适的荧光基团以及优化其化学结构以提高性能（如亮度、稳定性）。
- 荧光标记技术：如何将荧光探针有效地连接到目标分子上，确保准确标记同时不影响其功能。
- 显微镜成像技术：利用共聚焦激光扫描显微镜等工具获取高分辨率图像。
随着纳米技术和生物工程技术的进步，新型多功能荧光探针不断涌现。它们不仅能够提供更精准的定位信息，还能实现多色多重标记分析，为复杂系统的研究提供强有力的支持。此外，在成像速度、信噪比等方面的技术革新也正逐步推进荧光成像技术向更加高效便捷的方向发展。
| 中文名称 | 英文名称 | CAS号 | 化学式 | 结构式图片 |
|---|---|---|---|---|
| 6-[荧光素-5(6)-甲酰氨基]-己酸-N-羟基琥珀酰亚胺酯 | fluorescein-5(6)-carboxamidocaproic acid N-hydroxysuccinimide ester | 148356-00-7 | C31H26N2O10 |
|
| 6-[(7-硝基-2,1,3-苯并恶二唑-4-基)氨基]己酸 | 6-(7-nitrobenzofurazan-4-ylamino)hexanoic acid | 88235-25-0 | C12H14N4O5 |
|
| 6-(2,4-二硝基苯胺基)己酸 N-琥珀酰亚氨基酯 | 6-(2,4-dinitrophenyl)aminohexanoic acid succinimidyl ester | 82321-04-8 | C16H18N4O8 |
|
| 5-羧基荧光素琥珀酰亚胺酯 | 5-carboxyfluorescein succinimidyl ester | 92557-80-7 | C25H15NO9 |
|
| 5-羧基四甲基罗丹明琥珀酰亚胺酯 | 5-carboxytetramethylrhodamine succinimidyl ester | 150810-68-7 | C29H25N3O7 |
|
| 5-羧基四甲基罗丹明 | 5-TAMRA | 91809-66-4 | C25H22N2O5 |
|
| 5-羧基-X-罗丹明琥珀酰亚胺酯(单一化合物) | 5-carboxy-X-rhodamine N-succinimidyl ester | 209734-74-7 | C37H33N3O7 |
|
| 5-(碘乙酰胺基)荧光素 | 5-(iodoacetamido)fluorescein | 63368-54-7 | C22H14INO6 |
|
| 5-(4,6-二氯三嗪)氨基荧光素 | 5-(4,6-dichlorotriazinyl)aminofluorescein | 51306-35-5 | C23H12Cl2N4O5 |
|
| 5(6)-羧基荧光素琥珀酰亚胺酯 | NHS-5(6)-fluorescein | 117548-22-8 | C25H15NO9*C25H15NO9 |
|
| 5(6)-羧基荧光素二乙酸酯 | 5(6)-Carboxyfluorescein diacetate | 124387-19-5 | C50H32O18 |
|
| 4-甲基伞型酮-beta-D-葡糖苷酸 | 4-methylumbelliferyl-β-D-glucuronide | 6160-80-1 | C16H16O9 |
|
| 4-[4-(二甲基氨基)苯偶氮]苯甲酸 N-丁二酰亚胺酯 | 4-[4-(dimethylamino)phenylazo]benzoic acid N-succinimidyl ester | 146998-31-4 | C19H18N4O4 |
|
| 4-(6-羧基-2-吲哚基)-4'-甲基-2,2'-(乙烯基二氧)二苯胺-N,N,N',N'-四乙酸四(乙酰氧基甲基)酯 | Indo-1 pentaacetoxymethyl ester | 112926-02-0 | C47H51N3O22 |
|
| 4-(6-乙酰甲氧-2,7-二氯-3-氧-9-夹氧杂蒽基)-4'-甲基-2,2'(乙基烯二氧)二苯胺-N,N,N',N'-四乙酸四(乙酰氧基甲基)酯 | Fluo 3-AM | 121714-22-5 | C48H46Cl2N2O21 |
|
| 3-氨基-8-叠氮-5-苯乙烯-6-苯基菲酚溴化物 | 8-Azido-5-ethyl-6-phenylphenanthridin-3(5H)-imine--hydrogen bromide (1/1) | 58880-05-0 | C21H18BrN5 |
|
| 3-(溴甲基)-2,6,7-三甲基-1H,5H-吡唑并[1,2-a]吡唑-1,5-二酮 | 9,10-dioxa-anti-(bromomethyl,methyl)(methyl,methyl)bimane | 74235-78-2 | C10H11BrN2O2 |
|
| 3-(6-甲氧基-1-喹啉基)丙磺酸内盐单水合物 | 6-(methoxy)-1-(3-sulfonatopropyl)-quinolinium | 83907-40-8 | C13H15NO4S |
|
| 3,3’-二庚基氧化碳菁碘化物 | (2E)-3-heptyl-2-[(Z)-3-(3-heptyl-1,3-benzoxazol-3-ium-2-yl)prop-2-enylidene]-1,3-benzoxazole;iodide | 53213-83-5 | C31H41IN2O2 |
|
| 3,3-二戊基草酸碳菁碘 | (2E)-3-pentyl-2-[(Z)-3-(3-pentyl-1,3-benzoxazol-3-ium-2-yl)prop-2-enylidene]-1,3-benzoxazole;iodide | 53213-81-3 | C27H33IN2O2 |
|
| 3,12-双[2-[(乙酰氧基)甲氧基]-2-氧代乙基]-6,9-二氧杂-3,12-二氮杂十四烷二酸1,14-双[(乙酰氧基)甲基]酯 | EGTA acetoxymethyl ester | 99590-86-0 | C26H40N2O18 |
|
| 10-乙酰基-3,7-二羟基吩嗪 | 10-acetyl-3,7-dihydroxyphenoxazine | 119171-73-2 | C14H11NO4 |
|
| 1,5-双(5-氧代-3-丙基异恶唑-4-基)五甲川氧杂菁 | (4Z)-4-[(2E,4E)-5-(5-oxo-3-propyl-2H-1,2-oxazol-4-yl)penta-2,4-dienylidene]-3-propyl-1,2-oxazol-5-one | 64724-75-0 | C17H20N2O4 |
|
| 1,3-二丁基-5-[5-(1,3-二丁基六氢-2,4,6-三氧代-5-嘧啶基)-2,4-戊二烯-1-亚基]-2,4,6(1H,3H,5H)嘧啶三酮 | Bis-(1,3-dibutylbarbituric acid)pentamethine oxonol | 63560-89-4 | C29H42N4O6 |
|
| 1,1’-双十八烷基-3,3,3’,3’-四甲基吲哚二碳菁高氯酸盐 | 2-[(2E,4E)-5-(3,3-dimethyl-1-octadecylindol-1-ium-2-yl)penta-2,4-dienylidene]-3,3-dimethyl-1-octadecylindole;perchlorate | 127274-91-3 | C61H99ClN2O4 |
|
| 1,1’,3,3,3’,3’-六甲基吲哚双碳菁碘 | 1,1',3,3,3',3'-hexamethylindodicarbocyanine iodide | 36536-22-8 | C27H31N2*I |
|
| (4E)-2-碘-4-[5-[6-(4-甲基哌嗪-1-基)-1H-苯并咪唑-2-基]-1,3-二氢苯并咪唑-2-亚基]环己-2,5-二烯-1-酮 | monoiodoHoechst 33258 | 106050-84-4 | C25H23IN6O |
|
| —— | 4-iodo-1-<5'-<5''-(4'''-methylpiperazin-1'''-yl)benzimidazol-2''-yl>benzimidazol-2'-yl>benzene | 158013-43-5 | C25H23IN6 |
|
| —— | 5-[2-[[2-[[2-[[2-[[2-[[2-[[2-[2-[[5-Amino-2-[2-[[2-[[4-[[4-(dimethylamino)phenyl]diazenyl]benzoyl]amino]-4-methylpentanoyl]amino]propanoylamino]-5-oxopentanoyl]amino]propanoylamino]-3-methylbutanoyl]amino]-5-(diaminomethylideneamino)pentanoyl]amino]-3-hydroxypropanoyl]amino]-3-hydroxypropanoyl]amino]-3-hydroxypropanoyl]amino]-5-(diaminomethylideneamino)pentanoyl]amino]ethylamino]naphthalene-1-sulfonic acid | 396716-14-6 | C70H104N22O18S |
|
| —— | L-Prolinamide, N-[4-[[4-(dimethylamino)phenyl]azo]benzoyl]-L-alpha-glutamyl-L-arginyl-L-norleucyl-L-phenylalanyl-L-leucyl-L-seryl-L-phenylalanyl-N-[2-[(5-sulfo-1-naphthalenyl)amino]ethyl]-(9CI) | 263718-22-5 | C76H98N16O15S |
|
| —— | N-(3-Methylbutanoyl)alanyl-N-[4-(difluoromethyl)-2-oxo-2H-1-benzopyran-7-yl]-alpha-asparagine | 219137-85-6 | C22H25F2N3O7 |
|
| —— | Benzenesulfonic acid,2'-(1,2-ethenediyl)bis(5-isothiocyanoto-, disodium salt | 67483-13-0 | C16H10N2NaO6S4+ |
|
| —— | Renin FRET Substrate I | 142988-22-5 | C90H120N22O16S |
|
| —— | chrysamine G | 6472-91-9 | C26H16N4O6*2Na |
|
| —— | 3-iodo-1-<5'-<5''-(4'''-methylpiperazin-1'''-yl)benzimidazol-2''-yl>benzimidazol-2'-yl>benzene | 158013-42-4 | C25H23IN6 |
|
| —— | 2-iodo-1-<5'-<5''-(4'''-methylpiperazin-1'''-yl)benzimidazol-2''-yl>benzimidazol-2'-yl>benzene | 158013-41-3 | C25H23IN6 |
|
| —— | cis-Merocyanine 540 | 62796-23-0 | C26H32N3O6S2*Na |
|
| —— | sodium 3,3'-<1-<(phenylamino)carbonyl>-3,4-tetrazolium>-bis(4-methoxy-6-nitro)benzenesulfonic acid | 111072-31-2 | C22H16N7O13S2*Na |
|
| —— | Bis-(1,3-diethylthiobarbituric acid)trimethine oxonol | 47623-98-3 | C19H24N4O4S2 |
|
| —— | N,N-bis(2-chloroethyl)-4-[3-[6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazol-2-yl]propyl]aniline | 178481-68-0 | C32H37Cl2N7 |
|
| —— | 2-<2-(3,5-di-tert-butyl-4-hydroxyphenyl)-5'(6')-benzimidazolyl>-5(6)-<1-(4-methylpiperazinyl)>benzimidazole | 129244-66-2 | C33H40N6O |
|
| —— | Fura 2am | 108964-32-5 | C44H47N3O24 |
|
| —— | Ac-IETD-AFC | 211990-57-7 | C31H38F3N5O12 |
|
| —— | sodium;3-[(2Z)-2-[(E)-4-(1,3-dibutyl-4,6-dioxo-2-sulfanylidene-1,3-diazinan-5-ylidene)but-2-enylidene]-1,3-benzoxazol-3-yl]propane-1-sulfonic acid | 62796-23-0 | C26H33N3NaO6S2+ |
|
| —— | N-Acetylleucyl-alpha-glutamylhistidyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-alpha-asparagine | 210345-03-2 | C33H38F3N7O11 |
|
| —— | N-Acetylisoleucyl-alpha-glutamylthreonyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-alpha-asparagine | 211990-57-7 | C31H38F3N5O12 |
|
| —— | Autocamtide 2 | 129198-88-5 | C65H118N22O20 |
|
| —— | 2'-(4-chloro-phenyl)-5-(4-methyl-piperazin-1-yl)-1<i>H</i>,1'<i>H</i>-[2,5']bibenzoimidazolyl | 23554-99-6 | C25H23ClN6 |
|
| —— | meta-Hoechst | 132869-83-1 | C25H24N6O |
|
| —— | 5-(iodoacetamido)fluorescein | 63368-54-7 | C22H14INO6 |
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