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    首页 分子通 按物质功能分类 生物 细胞培养

    细胞培养

    细胞培养 定义

    细胞培养是指在人工控制条件下对离体的动物或植物细胞进行生长、繁殖和维持的过程。这一技术广泛应用于生物医药、生物工程以及基础研究等领域。

    应用领域
    • 生物医药：药物研发、细胞治疗、基因编辑等。
    • 农业与生物技术：转基因生物开发、微生物发酵生产抗生素及其他生物制品。
    • 诊断试剂制造：用于制备抗体和抗原，以支持疾病诊断。
    • 组织工程：构建人工器官或组织。
    主要步骤
    1. 细胞采集：从生物体中获取细胞样本。
    2. 细胞培养基配制：根据不同的研究目的选择合适的培养基配方。通常包含基础培养基、血清、氨基酸、维生素、生长因子等多种成分。
    3. 细胞接种与培养条件设置：
      • 温度控制（一般为37℃）；
      • 气体环境调控（如95%空气和5%二氧化碳）；
      • 酸碱度调整（pH值通常维持在7.2-7.4之间）。
    4. 监测与维护：定期观察细胞生长状态，必要时补充营养物质或更换培养基。
    关键技术
    • 无血清培养：减少传统动物源性成分带来的生物安全问题。
    • 多能干细胞的体外培养：对于再生医学和个性化医疗具有重要意义。
    • 高通量筛选平台构建：加快药物开发过程中的化合物测试速度。

    细胞培养作为连接基础科研与临床应用之间的桥梁，在推动生命科学领域进步方面发挥着不可替代的作用。

    下级分类

    相关化合物

    中文名称 英文名称 CAS号 化学式 结构式图片
    —— Dehydroabietylamin 1446-61-3 C20H31N
    Dehydroabietylamin
    —— Fmoc-L-Hgn(Trt)-OH 1263046-43-0 C40H36N2O5
    Fmoc-L-Hgn(Trt)-OH
    —— cis-3-(tert-Butoxycarbonylamino) cyclobutanecarboxylic acid 1008773-79-2 C10H17NO4
    cis-3-(tert-Butoxycarbonylamino) cyclobutanecarboxylic acid
    —— 4-Fluorotropacocaine 172883-97-5 C15H18FNO2
    4-Fluorotropacocaine
    —— 1-(2,4,6-trimethoxybenzyl)piperazine 113698-83-2 C14H22N2O3*2ClH
    1-(2,4,6-trimethoxybenzyl)piperazine
    —— (alphaR,betaR)-alpha-(2,4-Difluorophenyl)-5-fluoro-beta-methyl-alpha-(1H-1,2,4-triazol-1-ylmethyl)-4-pyrimidineethanol 239807-03-5 C16H14F3N5O
    (alphaR,betaR)-alpha-(2,4-Difluorophenyl)-5-fluoro-beta-methyl-alpha-(1H-1,2,4-triazol-1-ylmethyl)-4-pyrimidineethanol
    —— 3-(4-Methoxyphenyl)morpholine hydrochloride 1171560-33-0 C11H16ClNO2
    3-(4-Methoxyphenyl)morpholine hydrochloride
    —— 1-β-Naphthyl-3-isopropylamino-propan-1-on 58753-54-1 C16H19NO
    1-β-Naphthyl-3-isopropylamino-propan-1-on
    —— 3,5-diamino-6-chloro-N-((E)-N'-(4-((E)-phenyldiazenyl)phenyl)carbamimidoyl)pyrazine-2-carboxamide 1620951-72-5 C18H16ClN9O
    3,5-diamino-6-chloro-N-((E)-N'-(4-((E)-phenyldiazenyl)phenyl)carbamimidoyl)pyrazine-2-carboxamide
    —— Formaldehyde sodium bisulfite hemihydrate 63148-74-3 C2H8Na2O9S2
    Formaldehyde sodium bisulfite hemihydrate
    —— N-formyl-Nle-Leu-Phe-Nle-Tyr-Lys 71901-21-8 C43H65N7O9
    N-formyl-Nle-Leu-Phe-Nle-Tyr-Lys
    —— Acid Anthracene Red G 1836-22-2 C17H12N2NaO9S2+
    Acid Anthracene Red G
    —— trans-3-((tert-butoxycarbonyl)amino)cyclobutane-1-carboxylic acid 939400-34-7 C10H17NO4
    trans-3-((tert-butoxycarbonyl)amino)cyclobutane-1-carboxylic acid
    —— 2-[(1R,3aS,4E,7aR)-4-[(2E)-2-[(3S,5R)-3,5-bis[[tert-butyl(dimethyl)silyl]oxy]-2-methylidenecyclohexylidene]ethylidene]-7a-methyl-2,3,3a,5,6,7-hexahydro-1H-inden-1-yl]propanal 112828-13-4 C34H60O3Si2
    2-[(1R,3aS,4E,7aR)-4-[(2E)-2-[(3S,5R)-3,5-bis[[tert-butyl(dimethyl)silyl]oxy]-2-methylidenecyclohexylidene]ethylidene]-7a-methyl-2,3,3a,5,6,7-hexahydro-1H-inden-1-yl]propanal
    —— Glycine, N,N'-[(1,1-dioxido-3H-2,1-benzoxathiol-3-ylidene)bis[(6-hydroxy-2,5-dimethyl-3,1-phenylene)methylene]]bis[N-(carboxymethyl)-, sodium salt (1:1) 29412-85-9 C33H35N2NaO13S
    Glycine, N,N'-[(1,1-dioxido-3H-2,1-benzoxathiol-3-ylidene)bis[(6-hydroxy-2,5-dimethyl-3,1-phenylene)methylene]]bis[N-(carboxymethyl)-, sodium salt (1:1)
    —— quinestrol 152-43-2 C25H32O2
    quinestrol
    —— pyridostatin 1085412-37-8 C31H32N8O5
    pyridostatin
    —— [(2S,3S,5S,8R,9S,10S,13S,14S,16S,17R)-3-hydroxy-10,13-dimethyl-16-(1-prop-2-enylpyrrolidin-1-ium-1-yl)-2-pyrrolidin-1-yl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl] acetate;bromide 1190105-65-7 Br*C32H53N2O3
    [(2S,3S,5S,8R,9S,10S,13S,14S,16S,17R)-3-hydroxy-10,13-dimethyl-16-(1-prop-2-enylpyrrolidin-1-ium-1-yl)-2-pyrrolidin-1-yl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl] acetate;bromide
    —— n-Tetradecenylsuccinic Anhydride 33806-58-5 C18H30O3
    n-Tetradecenylsuccinic Anhydride
    —— cis-4-(Trifluoromethyl)cyclohexanecarboxylic acid 1202578-27-5 C8H11F3O2
    cis-4-(Trifluoromethyl)cyclohexanecarboxylic acid
    —— CYCLO(ARG-GLY-ASP-D-PHE-VAL) 137813-35-5 C26H38N8O7
    CYCLO(ARG-GLY-ASP-D-PHE-VAL)
    —— 2-(1,3-Dithiol-2-ylidene)-1,3-dithiole;ditetrafluoroborate 55492-86-9 C18H12B2F8S12-2
    2-(1,3-Dithiol-2-ylidene)-1,3-dithiole;ditetrafluoroborate
    —— meso-tetrakis-(2,6-dichlorophenyl)porphyrin 37083-37-7 C44H22Cl8N4
    meso-tetrakis-(2,6-dichlorophenyl)porphyrin
    —— potassium hexahydroxoplatinate 12285-90-4 H6O6Pt*2K
    potassium hexahydroxoplatinate
    —— 3-methylamino-5,6-dihydro-2H-1,4-thiazin-2-one oxime hydrochloride 112233-23-5 C5H9N3OS*ClH
    3-methylamino-5,6-dihydro-2H-1,4-thiazin-2-one oxime hydrochloride
    —— dirhodium tetracetate dihydrate 29998-99-0 C8H16O10Rh2
    dirhodium tetracetate dihydrate
    —— tetracyclo[6.2.1.13,6.02,7]dodeca-4-ene 21635-90-5 C12H16
    tetracyclo[6.2.1.13,6.02,7]dodeca-4-ene
    —— O-tricyclo[5.2.1.02,6]dec-9-yl dithiocarbonate potassium salt 83373-60-8 C11H15OS2*K
    O-tricyclo[5.2.1.02,6]dec-9-yl dithiocarbonate potassium salt
    —— 2,5-Cyclohexadiene-1,4-dione, 2,3,5,6-tetrahydroxy-, sodium salt (1:2) 1887-02-1 C6H4NaO6
    2,5-Cyclohexadiene-1,4-dione, 2,3,5,6-tetrahydroxy-, sodium salt (1:2)
    —— Phenol, 2,3,4,6-tetrachloro-, sodium salt (1:1) 131-61-3 C6H2Cl4NaO+
    Phenol, 2,3,4,6-tetrachloro-, sodium salt (1:1)
    —— Tetroquinone disodium derivative 1887-02-1 C6H4NaO6+
    Tetroquinone disodium derivative
    —— Disodium sebacate 17265-14-4 C10H16Na2O4
    Disodium sebacate
    —— [(1S,7S,8S,8aS)-8-[2-[(2R,4R)-4-hydroxy-6-oxooxan-2-yl]ethyl]-3,7-dimethyl-6-oxo-2,7,8,8a-tetrahydro-1H-naphthalen-1-yl] 2,2-dimethylbutanoate 130468-11-0 C25H36O6
    [(1S,7S,8S,8aS)-8-[2-[(2R,4R)-4-hydroxy-6-oxooxan-2-yl]ethyl]-3,7-dimethyl-6-oxo-2,7,8,8a-tetrahydro-1H-naphthalen-1-yl] 2,2-dimethylbutanoate
    —— Einecs 249-772-2 29681-21-8 C9H20O4
    Einecs 249-772-2
    —— 2,5-Furandione, dihydro-3-(tetradecenyl)- 33806-58-5 C18H30O3
    2,5-Furandione, dihydro-3-(tetradecenyl)-
    —— N-[(benzyloxy)carbonyl]leucylleucine 7801-71-0 C20H30N2O5
    N-[(benzyloxy)carbonyl]leucylleucine
    —— cis-3,3,5-Trimethylcyclohexyl acetate 24691-16-5 C11H20O2
    cis-3,3,5-Trimethylcyclohexyl acetate
    —— Bithionolate sodium 6385-58-6 C12H4Cl4Na2O2S
    Bithionolate sodium
    —— Einecs 200-684-2 68-34-8 C13H13NO2S
    Einecs 200-684-2
    —— 2-Amino-3-hydroxyoctadec-4-en-1-yl 2-(trimethylammonio)ethyl phosphate 1670-26-4 C23H49N2O5P
    2-Amino-3-hydroxyoctadec-4-en-1-yl 2-(trimethylammonio)ethyl phosphate
    —— β-Methoxy-α-(3,4,5-trimethoxy-benzyliden)-propionitril 7520-69-6 C14H17NO4
    β-Methoxy-α-(3,4,5-trimethoxy-benzyliden)-propionitril
    —— 1-(2-Chloroethyl)-1-nitroso-3-(2,3,4,5-tetrahydroxycyclopentyl)urea 64218-24-2 C8H14ClN3O6
    1-(2-Chloroethyl)-1-nitroso-3-(2,3,4,5-tetrahydroxycyclopentyl)urea
    —— (R,Z)-Butyl 12-hydroxyoctadec-9-enoate 151-13-3 C22H42O3
    (R,Z)-Butyl 12-hydroxyoctadec-9-enoate
    —— Fmoc-D-Tyr(OAll)-OH 204269-35-2 C27H25NO5
    Fmoc-D-Tyr(OAll)-OH
    —— triisopropyl benzene-1,2,4-tricarboxylate 1528-53-6 C18H24O6
    triisopropyl benzene-1,2,4-tricarboxylate
    —— 1-Ethoxy-1-cis-hex-3-enoxy-ethan 28069-74-1 C10H20O2
    1-Ethoxy-1-cis-hex-3-enoxy-ethan
    —— histidylglycine 2578-58-7 C8H12N4O3
    histidylglycine
    —— endo-5,6-Trimethylen-2-norbornanon 13380-94-4 C10H14O
    endo-5,6-Trimethylen-2-norbornanon
    —— exo-Trimethylen-norbornanon-(2) 13380-94-4 C10H14O
    exo-Trimethylen-norbornanon-(2)
    —— 1,1,3e-Trimethylcyclohexan 3073-66-3 C9H18
    1,1,3e-Trimethylcyclohexan