细胞培养
细胞培养
定义
细胞培养是指在人工控制条件下对离体的动物或植物细胞进行生长、繁殖和维持的过程。这一技术广泛应用于生物医药、生物工程以及基础研究等领域。
应用领域- 生物医药：药物研发、细胞治疗、基因编辑等。
- 农业与生物技术：转基因生物开发、微生物发酵生产抗生素及其他生物制品。
- 诊断试剂制造：用于制备抗体和抗原，以支持疾病诊断。
- 组织工程：构建人工器官或组织。
- 细胞采集：从生物体中获取细胞样本。
- 细胞培养基配制：根据不同的研究目的选择合适的培养基配方。通常包含基础培养基、血清、氨基酸、维生素、生长因子等多种成分。
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细胞接种与培养条件设置：
- 温度控制（一般为37℃）；
- 气体环境调控（如95%空气和5%二氧化碳）；
- 酸碱度调整（pH值通常维持在7.2-7.4之间）。
- 监测与维护：定期观察细胞生长状态，必要时补充营养物质或更换培养基。
- 无血清培养：减少传统动物源性成分带来的生物安全问题。
- 多能干细胞的体外培养：对于再生医学和个性化医疗具有重要意义。
- 高通量筛选平台构建：加快药物开发过程中的化合物测试速度。
细胞培养作为连接基础科研与临床应用之间的桥梁，在推动生命科学领域进步方面发挥着不可替代的作用。
| 中文名称 | 英文名称 | CAS号 | 化学式 | 结构式图片 |
|---|---|---|---|---|
| —— | N-Fmoc-N-methyl-L-methionine | 84000-12-4 | C21H23NO4S |
|
| —— | 3,6-di(4-chlorophenyl)-1,4-diketopyrrolo[3,4-c]pyrrole | 84632-65-5 | C18H8Cl2N2O2 |
|
| —— | 12-hydroxyabieta-8,11,13-trien-20-oic acid | 67494-15-9 | C20H28O3 |
|
| —— | 2-(carbobenzyloxy)-2-azabicyclo<2.2.1>heptan-5-one | 140927-13-5 | C14H15NO3 |
|
| —— | methyl D-(+)-α-(2-thienylethylamino)-α-(2-chlorophenyl)acetate hydrochloride | 141109-19-5 | C15H16ClNO2S*ClH |
|
| —— | (1H-1,2,3-triazol-4-yl)methanamine hydrochloride | 118724-05-3 | C3H6N4*ClH |
|
| —— | 2-Amino-3-hydroxyoctadec-4-en-1-yl 2-(trimethylazaniumyl)ethyl phosphate | 1670-26-4 | C23H50N2O5P+ |
|
| —— | (1R,4R,6R,7Z,17R)-N-cyclopropylsulfonyl-17-[7-methoxy-8-methyl-2-(4-propan-2-yl-1,3-thiazol-2-yl)quinolin-4-yl]oxy-13-methyl-2,14-dioxo-3,13-diazatricyclo[13.3.0.04,6]octadec-7-ene-4-carboxamide | 923604-59-5 | C38H47N5O7S2 |
|
| —— | Pubchem_53384435 | 127923-85-7 | C43H72N2O33 |
|
| —— | Phenyl 3,4,6-tri-O-acetyl-2-deoxy-1-thio-2-{[(2,2,2-trichloroethoxy)carbonyl]amino}-alpha-L-allopyranoside | 278784-83-1 | C21H24Cl3NO9S |
|
| —— | 1,3-Dichloro-5-[2-(4-chlorophenyl)ethenyl]benzene | 688348-25-6 | C14H9Cl3 |
|
| —— | 1,3-Bis(phenylmethoxy)-5-[2-(4-phenylmethoxyphenyl)ethenyl]benzene | 89946-06-5 | C35H30O3 |
|
| —— | N-{4-[4-(piperidinomethyl)pyridyl-2-oxy]-cis-2-butene}phthalimide Maleate | 146447-26-9 | C27H29N3O7 |
|
| —— | BDH succinate salt | 183388-64-9 | C50H70N4O10 |
|
| —— | 2-({(2S,4S,5S)-2,3,4-Tris(benzyloxy)-5-[(benzyloxy)methyl]-5-hydroxycyclohexyl}amino)propane-1,3-diol | 115250-39-0 | C38H45NO7 |
|
| —— | Boc-D-Arg(Mts)-OH Cha | 68262-72-6 | C26H45N5O6S |
|
| —— | Simeprevir; TMC435 | 923604-59-5 | C38H47N5O7S2 |
|
| —— | 5-methyl-3-(piperidin-4-yl)-1H-indole hydrochloride salt | 149669-44-3 | C14H18N2*ClH |
|
| —— | z-Ala-ala-leu-p-nitroanilid | 61043-33-2 | C26H33N5O7 |
|
| —— | 2-(4-((4-(Piperidin-1-ylmethyl)pyridin-2-yl)oxy)but-2-en-1-yl)isoindoline-1,3-dione but-2-enedioate | 146447-26-9 | C27H29N3O7 |
|
| —— | (Z)-but-2-enedioic acid;2-[(E)-4-[4-(piperidin-1-ylmethyl)pyridin-2-yl]oxybut-2-enyl]isoindole-1,3-dione | 146447-26-9 | C27H29N3O7 |
|
| —— | (1R,4R,7Z,15R,17R)-N-cyclopropylsulfonyl-17-[7-methoxy-8-methyl-2-(4-propan-2-yl-1,3-thiazol-2-yl)quinolin-4-yl]oxy-13-methyl-2,14-dioxo-3,13-diazatricyclo[13.3.0.04,6]octadec-7-ene-4-carboxamide | 923604-59-5 | C38H47N5O7S2 |
|
| —— | tert-Butyl (3S,5R,6E)-7-[4-(4-fluorophenyl)-2-[(methanesulfonyl)(methyl)amino]-6-(propan-2-yl)pyrimidin-5-yl]-3,5-dihydroxyhept-6-enoate | 615263-60-0 | C26H36FN3O6S |
|
| —— | (4S)-3-hydroxy-6-methyl-4-[(2-methylpropan-2-yl)oxycarbonylamino]heptanoic acid | 58521-49-6 | C13H25NO5 |
|
| —— | (S)-2-((((9H-fluoren-9-yl)methoxy)carbonyl)amino)-3-(4-(prop-2-yn-1-yloxy)phenyl)propanoic acid | 1204595-05-0 | C27H23NO5 |
|
| —— | cis-3,3,5-trimethylcyclohexanol acetate | 24691-16-5 | C11H20O2 |
|
| —— | 1-chloro-1-phenyl-heptane | 71434-47-4 | C13H19Cl |
|
| —— | 3-acetylisoquinoline | 91544-03-5 | C11H9NO |
|
| —— | 9H-fluoren-9-ylmethyl [(1S)-1-(4-tert-butoxybenzyl)-2-hydroxyethyl]carbamate | 187526-99-4 | C28H31NO4 |
|
| —— | CTV1 | 924284-35-5 | C39H30O6 |
|
| —— | (Z)-Resveratrol 4'-glucoside | 38963-95-0 | C20H22O8 |
|
| —— | Titanium, tetrakis(2,4-pentanedionato-kappaO2,kappaO4)- | 17501-79-0 | C20H28O8Ti |
|
| —— | Luminol sodium salt | 20666-12-0 | C8H7N3NaO2 |
|
| —— | 4,6-Dimethyl-1 | 17501-79-0 | C5H8O2Ti |
|
| —— | 4-Oxopent-2-en-2-olate;titanium(4+) | 17501-79-0 | C20H28O8Ti |
|
| —— | [6,6]-phenyl-C<sub>61</sub>-butyric acid butyl ester | 571177-66-7 | C75H20O2 |
|
| —— | S-[2-(Diethylamino)ethyl] o-methyl methylphosphonothioate | 2043-00-7 | C8H20NO2PS |
|
| —— | acenaphthenequinone dioxime | 1932-08-7 | C12H8N2O2 |
|
| —— | Potassium;palladium(2+);tetrachloride | 10025-98-6 | Cl4KPd- |
|
| —— | (+)-5(S)-Hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoic acid | 70608-72-9 | C20H32O3 |
|
| —— | sodium 2,4,5-trichlorophenolate | 136-32-3 | C6H2Cl3O*Na |
|
| —— | 8-benzyl-8-azabicyclo[3.2.1]octan-3-one hydrochloride | 83393-23-1 | C14H17NO*ClH |
|
| —— | Methyl 3-(cyclopropylamino)-2-(2,4-dichloro-5-fluorobenzoyl)acrylate | 105392-26-5 | C14H12Cl2FNO3 |
|
| —— | 5-[2-propyloxy-5-(1-methyl-2-pyrrolidinylethylamidosulfonyl)phenyl]-1-methyl-3-propyl-1,6-dihydro-7H-pyrazolo(4,3-d)pyrimidine-7-one | 268203-93-6 | C25H36N6O4S |
|
| —— | tribenzylsilane | 1747-92-8 | C21H22Si |
|
| —— | O-(N-acetyl-alpha-neuraminosyl)-(2->3)-O-beta-delta-galactopyranosyl-(1->4)-O-[6-deoxy-alpha-L-galactopyranosyl-(1->3)]-O-2-(acetylamino)-2-deoxy-beta-delta-glucopyranosyl-(1->3)-O-beta-delta-galactopyranosyl-(1->4)-D-Glucose | 127923-85-7 | C43H72N2O33 |
|
| —— | sodium valerate | 6106-41-8 | C5H9O2*Na |
|
| —— | 4(3H)-oxo-5-iodo-7H-pyrrolo<2,3-d>pyrimidine | 135352-71-5 | C6H4IN3O |
|
| —— | 17265-14-4 | C10H18NaO4 |
|
|
| —— | 5-amino-2,3-dihydro-1,4-phthalazinedione sodium | 20666-12-0 | C8H6N3O2*Na |
|
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