Phosphoric acid mono-[3-(1H-imidazol-4-yl)-2-oxo-propyl] ester | 99979-59-6

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 有机磷酸及其衍生物
• 英文名称: Phosphoric acid mono-[3-(1H-imidazol-4-yl)-2-oxo-propyl] ester
• 英文别名: 3-(Imidazol-4-yl)-2-oxopropyl phosphate；[3-(1H-imidazol-5-yl)-2-oxopropyl] dihydrogen phosphate
• CAS: 99979-59-6
• 化学式: C₆H₉N₂O₅P
• 分子量: 220.122
• InChiKey: YCFFMSOLUMRAMD-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -2
• 重原子数：
  14
• 可旋转键数：
  5
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.33
• 拓扑面积：
  113
• 氢给体数：
  3
• 氢受体数：
  6

反应信息

• 作为反应物：
  描述：

  Phosphoric acid mono-[3-(1H-imidazol-4-yl)-2-oxo-propyl] ester 在 alpha-酮戊二酸 、 L-谷氨酸 、 histidinol phosphate aminotransferase 作用下， 生成 L-组氨醇磷酸酯
  参考文献：
  名称：

  （βα）8-桶酶随机库的文库选择导致基因表达的意外诱导。

  摘要：

  通过结合体内随机诱变和选择的方法，测试了经常遇到的（βα ）8桶折叠获得新功能的潜力。为此，对编码52种不同的磷酸盐结合（βα ）8桶蛋白的基因进行易错PCR，并将其克隆到表达质粒中。所得的混合库用于转化不同的营养缺陷型大肠杆菌菌株，每个菌株均缺乏具有含磷酸盐底物的酶。将不同的转化子铺板在基本培养基上后，仅观察到两种菌株的生长，这两种菌株都缺少丝氨酸磷酸酶SerB或磷酸丝氨酸氨基转移酶SerC的基因。大肠杆菌的相同突变体基因楠E（编码推定Ñ -acetylmannosamine -6-磷酸2-差向异构酶）和PDX Ĵ（编码吡哆醇5'-磷酸合酶）负责抢救两个Δ SER B和Δ SER C.出乎意料的是，补充NANE和PdxJ的变种不能在体外催化SerB或SerC反应。取而代之的是，RT-qPCR，RNAseq和转录组分析表明，它们通过寻求内源性大肠杆菌的帮助而挽救了这些缺失。通过组氨酸操纵子

  DOI：

  10.1021/acs.biochem.9b00579
• 作为产物：
  描述：

  alpha-酮戊二酸 、 L-组氨醇磷酸酯 在 磷酸吡哆醛 、 histidinol phosphate aminotransferase Rv₂₂₃₁c 、 sodium chloride 作用下， 以 aq. buffer 为溶剂， 生成 L-谷氨酸 、 Phosphoric acid mono-[3-(1H-imidazol-4-yl)-2-oxo-propyl] ester
  参考文献：
  名称：

  10.1007/s00018-024-05200-8

  摘要：

  AbstractNitrogen metabolism of M. tuberculosis is critical for its survival in infected host cells. M. tuberculosis has evolved sophisticated strategies to switch between de novo synthesis and uptake of various amino acids from host cells for metabolic demands. Pyridoxal phosphate-dependent histidinol phosphate aminotransferase-HspAT enzyme is critically required for histidine biosynthesis. HspAT is involved in metabolic synthesis of histidine, phenylalanine, tyrosine, tryptophan, and novobiocin. We showed that M. tuberculosis Rv2231c is a conserved enzyme with HspAT activity. Rv2231c is a monomeric globular protein that contains α-helices and β-sheets. It is a secretory and cell wall-localized protein that regulates critical pathogenic attributes. Rv2231c enhances the survival and virulence of recombinant M. smegmatis in infected RAW264.7 macrophage cells. Rv2231c is recognized by the TLR4 innate immune receptor and modulates the host immune response by suppressing the secretion of the antibacterial pro-inflammatory cytokines TNF, IL-12, and IL-6. It also inhibits the expression of co-stimulatory molecules CD80 and CD86 along with antigen presenting molecule MHC-I on macrophage and suppresses reactive nitrogen species formation, thereby promoting M2 macrophage polarization. Recombinant M. smegmatis expressing Rv2231c inhibited apoptosis in macrophages, promoting efficient bacterial survival and proliferation, thereby increasing virulence. Our results indicate that Rv2231c is a moonlighting protein that regulates multiple functions of M. tuberculosis pathophysiology to increase its virulence. These mechanistic insights can be used to better understand the pathogenesis of M. tuberculosis and to design strategies for tuberculosis mitigation.

  DOI：

  10.1007/s00018-024-05200-8

文献信息

• Sequence-determined DNA fragments and corresponding polypeptides encoded thereby
  申请人：Ceres Incorporated

  公开号：EP1033405A2

  公开（公告）日：2000-09-06

  The present invention provides DNA molecules that constitute fragments of the genome of a plant, and polypeptides encoded thereby. The DNA molecules are useful for specifying a gene product in cells, either as a promoter or as a protein coding sequence or as an UTR or as a 3' termination sequence, and are also useful in controlling the behavior of a gene in the chromosome, in controlling the expression of a gene or as tools for genetic mapping, recognizing or isolating identical or related DNA fragments, or identification of a particular individual organism, or for clustering of a group of organisms with a common trait. 0Arabidopsis DNA is used in the present experiment, but the procedure is a general one.

  本发明提供了构成植物基因组片段的 DNA 分子及其编码的多肽。这些 DNA 分子可作为启动子或蛋白质编码序列或 UTR 或 3'终止序列，用于指定细胞中的基因产物，也可用于控制染色体中基因的行为，控制基因的表达，或作为基因绘图、识别或分离相同或相关 DNA 片段、或识别特定生物个体、或对具有共同性状的生物群体进行聚类的工具。 本实验中使用的是拟南芥 DNA，但这是一个通用程序。
• Probe compound for detecting and isolating enzymes and means and methods using the same
  申请人：Helmholtz-Zentrum für Infektionsforschung GmbH

  公开号：EP2230312A1

  公开（公告）日：2010-09-22

  The present invention relates to a probe compound that can comprise any substrate or metabolite of an enzymatic reaction in addition to an indicator component, such as, for example, a fluorescence dye, or the like. Moreover, the present invention relates to means for detecting enzymes in form of an array, which comprises any number of probe compounds of the invention which each comprise a different metabolite of interconnected metabolites representing the central pathways in all forms of life. Moreover, the present invention relates to a method for detecting enzymes involving the application of cell extracts or the like to the array of the invention which leads to reproducible enzymatic reactions with the substrates. These specific enzymatic reactions trigger the indicator (e.g. a fluorescence signal) and bind the enzymes to the respective cognate substrates. Moreover, the invention relates to means for isolating enzymes in form of nanoparticles coated with the probe compound of the invention. The immobilisation of the cognate substrates or metabolites on the surface of nanoparticles by means of the probe compounds allows capturing and isolating the respective enzyme, e.g. for subsequent sequencing.

  本发明涉及一种探针化合物，它可以包括酶反应的任何底物或代谢物，此外还包括指示成分，例如荧光染料或类似物。此外，本发明还涉及以阵列形式检测酶的方法，该阵列由任意数量的本发明探针化合物组成，每种探针化合物由代表所有生命形式中中心途径的相互关联的代谢物中的不同代谢物组成。此外，本发明还涉及一种检测酶的方法，该方法涉及将细胞提取物或类似物应用于本发明的阵列，从而导致与底物发生可重复的酶反应。这些特定的酶反应会触发指示剂（如荧光信号），并将酶与各自的同源底物结合。此外，本发明还涉及以涂覆有本发明探针化合物的纳米颗粒形式分离酶的方法。通过探针化合物将同源底物或代谢物固定在纳米颗粒表面，可以捕获和分离相应的酶，例如用于后续测序。
• Compositions and methods for modeling saccharomyces cerevisiae metabolism
  申请人：The Regents of The University of California

  公开号：EP2463654A1

  公开（公告）日：2012-06-13

  The invention provides an in silica model for determining a S. cerevisiae physiological function. The model includes a data structure relating a plurality of S. cerevisiae reactants to a plurality of S. cerevisiae reactions, a constraint set for the plurality of S. cerevisiae reactions, and commands for determining a distribution of flux through the reactions that is predictive of a S. cerevisiae physiological function. A model of the invention can further include a gene database containing information characterizing the associated gene or genes. The invention further provides methods for making an in silica S. cerevisiae model and methods for determining a S. cerevisiae physiological function using a model of the invention. The invention provides an in silica model for determining a S. cerevisiae physiological function. The model includes a data structure relating a plurality of S. cerevisiae reactants to a plurality of S. cerevisiae reactions, a constraint set for the plurality of S. cerevisiae reactions, and commands for determining a distribution of flux through the reactions that is predictive of a S. cerevisiae physiological function. A model of the invention can further include a gene database containing information characterizing the associated gene or genes. The invention further provides methods for making an in silica S. cerevisiae model and methods for determining a S. cerevisiae physiological function using a model of the invention.

  本发明提供了一种用于确定酿酒葡萄孢菌生理功能的硅胶模型。该模型包括一个将多个酿酒葡萄孢反应物与多个酿酒葡萄孢反应相关联的数据结构、多个酿酒葡萄孢反应的约束集，以及用于确定通过反应的通量分布的指令，该通量分布可预测酿酒葡萄孢的生理功能。本发明的模型可进一步包括一个基因数据库，其中包含表征相关基因的信息。本发明进一步提供了硅胶中酿酒葡萄孢模型的制作方法和使用本发明模型确定酿酒葡萄孢生理功能的方法。本发明提供了一种用于确定酿酒葡萄孢菌生理功能的硅胶模型。该模型包括将多个酿酒葡萄孢反应物与多个酿酒葡萄孢反应相关联的数据结构、多个酿酒葡萄孢反应的约束集，以及用于确定通过反应的通量分布的指令，该通量分布可预测酿酒葡萄孢的生理功能。本发明的模型可进一步包括一个基因数据库，其中包含表征相关基因的信息。本发明进一步提供了制作硅胶葡萄孢菌模型的方法和使用本发明模型确定葡萄孢菌生理功能的方法。
• 25324, 50287, 28899, 47007, and 42967 transferase family members and uses therefor
  申请人：——

  公开号：US20020019030A1

  公开（公告）日：2002-02-14

  The invention provides isolated nucleic acids molecules, designated transferase nucleic acid molecules, which encode novel transferase family members. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing transferase nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a transferase gene has been introduced or disrupted. The invention still further provides isolated transferase proteins, fusion proteins, antigenic peptides and anti-transferase antibodies. Diagnostic methods utilizing compositions of the invention are also provided.

  本发明提供了编码新型转移酶家族成员的分离核酸分子，称为转移酶核酸分子。本发明还提供了反义核酸分子、含有转移酶核酸分子的重组表达载体、导入表达载体的宿主细胞以及导入或破坏了转移酶基因的非人类转基因动物。本发明还进一步提供了分离的转移酶蛋白、融合蛋白、抗原肽和抗转移酶抗体。本发明还提供了利用本发明组合物的诊断方法。
• Lactobacillus rhamnosus polynucleotides, polypeptides and methods for using them
  申请人：——

  公开号：US20020159976A1

  公开（公告）日：2002-10-31

  Novel polynucleotides isolated from Lactobacillus rhamnosus, as well as probes and primers, genetic constructs comprising the polynucleotides, biological materials, including plants, microorganisms and multicellular organisms incorporating the polynucleotides, polypeptides expressed by the polynucleotides, and methods for using the polynucleotides and polypeptides are disclosed.

  从 鼠李糖乳杆菌、 以及探针和引物、包含多核苷酸的基因构建体、生物材料（包括包含多核苷酸的植物、微生物和多细胞生物）、由多核苷酸表达的多肽以及使用多核苷酸和多肽的方法均已公开。