3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase | 37277-69-3

• 分子结构分类: 转移酶 - 糖基转移酶 - 己糖基转移酶
• 英文名称: 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase
• 英文别名: (Lea)-dependent (alpha-3/4)-fucosyltransferase；alpha(1,3/1,4) fucosyltransferase III；alpha-(1->4)-L-fucosyltransferase；alpha-4-L-fucosyltransferase；beta-acetylglucosaminylsaccharide fucosyltransferase；FucT-II；Lewis alpha-(1->3/4)-fucosyltransferase；Lewis blood group alpha-(1->3/4)-fucosyltransferase；Lewis(Le) blood group gene-dependent alpha-(1->3/4)-L-fucosyltransferase；blood group Lewis alpha-4-fucosyltransferase；blood-group substance Lea-dependent fucosyltransferase；guanosine diphosphofucose-beta-acetylglucosaminylsaccharide 4-alpha-L-fucosyltransferase；guanosine diphosphofucose-glycoprotein 4-alpha-L-fucosyltransferase；guanosine diphosphofucose-glycoprotein 4-alpha-fucosyltransferase；3-alpha-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase；GDP-beta-L-fucose:3-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R 4I-alpha-L-fucosyltransferase；GDP-L-fucose:3-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R 4I-alpha-L-fucosyltransferase；Lewis α-(1->3/4)-fucosyltransferase
• CAS: 37277-69-3

反应信息

• 作为试剂：
  描述：

  methyl 8-{[beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosamyl]oxy}nonanoate 、 5`-二磷酸鸟嘌呤核苷-岩藻糖二钠盐 在 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase HEPES buffer 、 manganese(ll) chloride 作用下， 以 水 为溶剂， 反应 0.5h， 生成 8-methoxycarbonyloctyl 2-acetamido-2-deoxy-4-O-(alpha-L-fucopyranosyl)-3-O-(beta-D-galactopyranosyl)-beta-D-glucopyranoside
  参考文献：
  名称：

  Recognition of β-d-Gal p-(1 → 3)-β-d-Glc pNAc-OR acceptor analogues by the Lewis α-(1 → 34)-fucosyltransferase from human milk

  摘要：

  The Lewis alpha-(1 --> 3/4)-frucosyltransferase (E.C. 2.4.1.65) transfers L-fucose from GDP-fucose to OH-4 of the Glc pNAc residue in the disaccharide beta-D-Gal p-(1 --> 3)-beta-D-Glc pNAc-OR [R = (CH2)(8)COOMe] (1) to give the Lewis-A blood group determinant beta-D-Gal p-(1 --> 3)-[alpha-L-Fuc p-(1 --> 4)]-beta-D-Glc pNAc-OR. Five deoxy analogues of 1, as well as its N-propionyl derivative, were chemically synthesized and kinetically evaluated as both substrates and inhibitors for the enzyme from human milk. The unmodified acceptor 1 had K-m = 640 mu M with V-max set arbitrarily to 100. The 6-deoxy (K-m = 400 mu M, V-max = 90) and N-propionyl compounds (K-m = 330 mu M, V-max = 170) remained excellent substrates while the 4-deoxy compound was a very weak competitive inhibitor with K-i = 9 mM. Deoxygenation of OH-2' and OH-4' (of the Gal residue) in 1 had little effect on the activity. The OH-6 group of the Gal residue proved to be critical for recognition by the enzyme since substitution of this group with hydrogen led to an inactive compound. (C) 1996 Elsevier Science Ltd.

  DOI：

  10.1016/0008-6215(96)00047-x